Effect of apoferritin surface-biomacromolecular modification on cellular uptake and inhibition of protein corona
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Date
2019-01-02
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Advisor
Referee
Mark
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Mendel University in Brno
Abstract
The effects of surface modifications (PEGylation and PASylation) of natural nanocarriers
based on apoferritin (FRT) were tested in this work. The main goals of performed
PEGylation/PASylation were decreased protein corona formation leading to better internalization of
drugs into diseased cells and therefore higher efficiency of treatment. The influence of created protein
coronas on the amount of internalized experimental drug ellipticine (Elli) was evaluated via fluorescence
microscopy. Various properties of these modified nanoparticles were studied, such as their cytotoxicity
or release kinetics of Elli. According to performed experiments, PAS-10 modification appeared as the
most appropriate surface modification.
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Peer-reviewed
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Published version
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en
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(C) Mendel University in Brno