Automated alignment method for coherence-controlled holographic microscope

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Date
2015-10-28
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Mark
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SPIE
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Abstract
Coherence-controlled holographic microscope (CCHM) was developed particularly for quantitative phase imaging and measurement of live cell dynamics, which is the proper subject of digital holographic microscopy (DHM). CCHM in low-coherence mode extends DHM in the study of living cells. However, this advantage is compensated by sensitivity of the system to easily become misaligned, which is a serious hindrance to wanted performance. Therefore, it became clear that introduction of a self-correcting system is inevitable. Accordingly, we had to devise a theory of a suitable control and design an automated alignment system for CCHM. The modulus of the reconstructed holographic signal was identified as a significant variable for guiding the alignment procedures. From this, we derived the original basic realignment three-dimensional algorithm, which encompasses a unique set of procedures for automated alignment that contains processes for initial and advanced alignment as well as long-term maintenance of microscope tuning. All of these procedures were applied to a functioning microscope and the tested processes were successfully validated. Finally, in such a way, CCHM is enabled to substantially contribute to study of biology, particularly of cancer cells in vitro.
V článku je popsaná automatická metoda ladění pro koherencí řízenou holografickou mikroskopii. Všechny popsané procedury byly aplikovány na fungující mikroskop a testované procesy byly úspěšně ověřeny.
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Peer-reviewed
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en
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Creative Commons Attribution 3.0 Unported
http://creativecommons.org/licenses/by/3.0/
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