Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration
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Date
2017-01-10
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ORCID
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Referee
Mark
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Publisher
Springer Nature
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Abstract
We describe a new procedure for the parallel mapping of selected metals in histologically characterized
tissue samples. Mapping is achieved via image registration of digital data obtained from two
neighbouring cryosections by scanning the first as a histological sample and subjecting the second
to laser ablation inductively coupled plasma mass spectrometry. This computer supported procedure
enables determination of the distribution and content of metals of interest directly in the chosen
histological zones and represents a substantial improvement over the standard approach, which
determines these values in tissue homogenates or whole tissue sections. The potential of the described
procedure was demonstrated in a pilot study that analysed Zn and Cu levels in successive development
stages of pig melanoma tissue using MeLiM (Melanoma-bearing-Libechov-Minipig) model. We
anticipate that the procedure could be useful for a complex understanding of the role that the spatial
distribution of metals plays within tissues affected by pathological states including cancer.
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Citation
Scientific Reports. 2017, vol. 7, issue 40169, p. 40169-1-40169-13.
https://www.nature.com/articles/srep40169
https://www.nature.com/articles/srep40169
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Peer-reviewed
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en