Soluble collagen dissolution and assembling in pressurized carbon dioxide water solutions

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dc.contributor.authorZubal, Lukášcs
dc.contributor.authorBonani, Waltercs
dc.contributor.authorManiglio, Devidcs
dc.contributor.authorCeccato, Riccardocs
dc.contributor.authorRenčiuk, Danielcs
dc.contributor.authorHampl, Alešcs
dc.contributor.authorMigliaresi, Claudiocs
dc.contributor.authorJančář, Josefcs
dc.contributor.authorVojtová, Lucycs
dc.coverage.issue2cs
dc.coverage.volume12cs
dc.date.accessioned2020-08-04T11:58:04Z
dc.date.available2020-08-04T11:58:04Z
dc.date.issued2018-02-01cs
dc.description.abstractDissolution and gelation procedures have a great influence on gelation time, microstructure and mechanical properties of reconstituted collagen products. We have investigated the dissolution of atelocollagen in CO2/water solutions at low temperature (4 degrees C) at different CO2 pressures (0.3-0.9 MPa), as well as gelation kinetics and physico-chemical properties of the hydrogel obtained after CO2 removal. Compared to conventional methods, the CO2-assisted technique resulted in faster soluble collagen dissolution and faster gelation into transparent gels characterized by thin 10 nm fibrils. Electrophoresis and CD spectroscopy demonstrated that the process did not denature the soluble collagen. The possibility to obtain collagen dissolution and gelation without the use of chemical agent other than water and CO2 makes this process particularly appealing for biomedical applications.en
dc.formattextcs
dc.format.extent159-170cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationEXPRESS POLYM LETT. 2018, vol. 12, issue 2, p. 159-170.en
dc.identifier.doi10.3144/expresspolymlett.2018.14cs
dc.identifier.issn1788-618Xcs
dc.identifier.other146700cs
dc.identifier.urihttp://hdl.handle.net/11012/84160
dc.language.isoencs
dc.publisherBudapest University of Technologycs
dc.relation.ispartofEXPRESS POLYM LETTcs
dc.relation.urihttp://www.expresspolymlett.com/letolt.php?file=EPL-0008576&mi=cdcs
dc.rights(C) Budapest University of Technologycs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1788-618X/cs
dc.subjectbiocompatible polymersen
dc.subjectcollagen fibrillogenesisen
dc.subjectstructural analysisen
dc.subjectviscoelastic propertiesen
dc.subjectprotein self-assemblyen
dc.titleSoluble collagen dissolution and assembling in pressurized carbon dioxide water solutionsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-146700en
sync.item.dbtypeVAVen
sync.item.insts2021.11.22 12:54:50en
sync.item.modts2021.11.22 12:26:00en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie materiálůcs
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé polymerní materiály a kompozitcs
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