Di-4-ANEPPS modulates electrical activity and progress of myocardial ischemia in rabbit isolated heart

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dc.contributor.authorRonzhina, Marinacs
dc.contributor.authorStračina, Tiborcs
dc.contributor.authorLacinová, Ľubicacs
dc.contributor.authorOndáčová, Katarínacs
dc.contributor.authorPavlovičová, Michaelacs
dc.contributor.authorMaršánová, Luciecs
dc.contributor.authorSmíšek, Radovancs
dc.contributor.authorJanoušek, Otocs
dc.contributor.authorFialová, Kateřinacs
dc.contributor.authorKolářová, Janacs
dc.contributor.authorNováková, Mariecs
dc.contributor.authorProvazník, Ivocs
dc.coverage.issue6cs
dc.coverage.volume12cs
dc.date.accessioned2021-09-09T14:56:47Z
dc.date.available2021-09-09T14:56:47Z
dc.date.issued2021-06-10cs
dc.description.abstractAims: Although voltage-sensitive dye di-4-ANEPPS is a common tool for mapping cardiac electrical activity, reported effects on electrophysiological parameters are rather. The main goals of the study were to reveal effects of the dye on rabbit isolated heart and to verify, whether rabbit isolated heart stained with di-4-ANEPPS is a suitable tool for myocardial ischemia investigation. Methods and Results: Study involved experiments on stained (n = 9) and non-stained (n = 11) Langendorff perfused rabbit isolated hearts. Electrophysiological effects of the dye were evaluated by analysis of various electrogram (EG) parameters using common paired and unpaired statistical tests. It was shown that staining the hearts with di-4-ANEPPS leads to only short-term sporadic prolongation of impulse conduction through atria and AV node. On the other hand, significant irreversible slowing of heart rate and ventricular conduction were found in stained hearts as compared to controls. In patch clamp experiments, significant inhibition of sodium current density was observed in differentiated NG108-15 cells stained by the dye. Although no significant differences in mean number of VPBs were found between the stained and the non-stained hearts in ischemia as well as in reperfusion, all abovementioned results indicate increased arrhythmogenicity. In isolated hearts during ischemia, prominent ischemic patterns appeared in the stained hearts with 3-4 min delay as compared to the non-stained ones. Moreover, the ischemic changes did not achieve the same magnitude as in controls even after 10 minutes of ischemia. It resulted in poor performance of ischemia detection by proposed EG parameters, as was quantified by receiver operating characteristics analysis. Conclusions: Our results demonstrate significant direct irreversible effect of di-4-ANEPPS on spontaneous heart rate and ventricular impulse conduction in rabbit isolated heart model. Particularly, this should be considered when di-4-ANEPPS is used in ischemia studies in rabbit. Delayed attenuated response of such hearts to ischemia might lead to misinterpretation of obtained results.en
dc.formattextcs
dc.format.extent1-15cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationFrontiers in Physiology. 2021, vol. 12, issue 6, p. 1-15.en
dc.identifier.doi10.3389/fphys.2021.667065cs
dc.identifier.issn1664-042Xcs
dc.identifier.other171652cs
dc.identifier.urihttp://hdl.handle.net/11012/201544
dc.language.isoencs
dc.publisherFrontierscs
dc.relation.ispartofFrontiers in Physiologycs
dc.relation.urihttps://www.frontiersin.org/articles/10.3389/fphys.2021.667065/fullcs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1664-042X/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectDi-4-ANEPPSen
dc.subjectRabbit isolated hearten
dc.subjectMyocardial Ischemiaen
dc.subjectElectrogram analysisen
dc.subjectPatch- clampen
dc.subjectVoltage-sensitive dyeen
dc.titleDi-4-ANEPPS modulates electrical activity and progress of myocardial ischemia in rabbit isolated hearten
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-171652en
sync.item.dbtypeVAVen
sync.item.insts2021.11.29 08:55:00en
sync.item.modts2021.11.29 08:15:11en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrstvícs
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