Now showing items 1-14 of 14

  • Determination of Histamine in Silages Using Nanomaghemite Core (-Fe2O3)-Titanium Dioxide Shell Nanoparticles Off-Line Coupled with Ion Exchange Chromatography 

    Cernei, Natalia Vladimirovna; Lacková, Zuzana; Guráň, Roman; Hynek, David; Skládanka, Jiří; Horký, Pavel; Zítka, Ondřej; Adam, Vojtěch (MDPI, 2016-09-12)
    The presence of biogenic amines is a hallmark of degraded food and its products. Herein, we focused on the utilization of magnetic nanoparticles off-line coupled with ion exchange chromatography with post-column ninhydrin ...
  • Effect of apoferritin surface-biomacromolecular modification on cellular uptake and inhibition of protein corona 

    Tesařová, Barbora; Dostálová, Simona; Charousová, Markéta; Škubalová, Zuzana; Guráň, Roman; Do, Tomáš; Adam, Vojtěch; Heger, Zbyněk (Mendel University in Brno, 2019-01-02)
    The effects of surface modifications (PEGylation and PASylation) of natural nanocarriers based on apoferritin (FRT) were tested in this work. The main goals of performed PEGylation/PASylation were decreased protein corona ...
  • Effects of stratospheric conditions on viability, metabolism and proteome of prokaryotic cells 

    chudobova, Dagmar; Číhalová, Kristýna; Jelínková, Pavlína; Zítka, Jan; Nejdl, Lukáš; Guráň, Roman; Klimánek, Martin; Adam, Vojtěch; Kizek, René (MDPI, 2015-09-09)
    The application of ultraviolet (UV) radiation to inhibit bacterial growth is based on the principle that the exposure of DNA to UV radiation results in the formation of cytotoxic lesions, leading to inactivation of ...
  • Exposure to 17 beta-Oestradiol Induces Oxidative Stress in the Non-Oestrogen Receptor Invertebrate Species Eisenia fetida 

    Heger, Zbyněk; Michálek, Petr; Guráň, Roman; Havelková, Barbora; Komínková, Markéta; Cernei, Natalia Vladimirovna; Richtera, Lukáš; Beklová, Miroslava; Adam, Vojtěch; Kizek, René (PLOS, 2015-12-22)
    Background The environmental impacts of various substances on all levels of organisms are under investigation. Among these substances, endocrine-disrupting compounds (EDCs) present a threat, although the environmental ...
  • MALDI MSI of MeLiM melanoma: Searching for differences in protein profiles 

    Guráň, Roman; Pompeiano Vaníčková, Lucie; Horák, Vratislav; Křížková, Soňa; Michálek, Petr; Heger, Zbyněk; Zítka, Ondřej; Adam, Vojtěch (PLOS, 2017-12-08)
    Background Treatment of advanced cutaneous melanoma remains challenging, and new data on melanoma biology are required. The most widely accepted criteria for the prognostic evaluation of melanoma are histopathological and ...
  • MALDI-TOF MSI method for determining spatial distribution of infection markers in pulmonary tissues of pigs 

    Do, Tomáš; Jarošová, Rea; Ilieva, Lada; Guráň, Roman; Ondráčková, Petra; Faldyna, Martin; Sládek, Zbyšek; Zítka, Ondřej (Mendel University in Brno, 2019-01-02)
    In recent years the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI-TOF MSI) is used for molecular mapping of diverse biomarkers such as proteins or peptides in animal/plant ...
  • Novel Vancomycin-Peptide Conjugate as Potent Antibacterial Agent against Vancomycin-Resistant Staphylococcus aureus 

    Jelínková, Pavlína; Šplíchal, Zbyněk; Jimenez Jimenez, Ana Maria; Haddad, Yazan Abdulmajeed Eyadh; Mazumdar, Aninda; Sur, Vishma Pratap; Milosavljević, Vedran; Kopel, Pavel; Buchtelová, Hana; Guráň, Roman; Zítka, Ondřej; Richtera, Lukáš; Hegerová, Dagmar; Heger, Zbyněk; Moulick, Amitava; Adam, Vojtěch (Dove Press, 2018-11-01)
    Background: Increase in vancomycin (Van)-resistant bacterial strains including vancomycin-resistant Staphylococcus aureus (VRSA) and lack of new effective antibiotics have become a formidable health problem. Materials and ...
  • Preparation of cryosections from frozen porcine pulmonary tissue for MALDI mass spectrometry imaging 

    Jarošová, Rea; Smolíková, Vendula; Dvořák, Marek; Guráň, Roman; Do, Tomáš; Ondráčková, Petra; Zítka, Ondřej; Sládek, Zbyšek (Mendel University in Brno, 2019-01-02)
    In last decades, a matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) was used for mapping the spatial distribution of different molecules, mainly peptides, proteins, lipids and metabolites, ...
  • Prostate tumor attenuation in the nu/nu murine model due to antisarcosine antibodies in folate targeted liposomes 

    Heger, Zbyněk; Polanská, Hana; Merlos Rodrigo, Miguel Ángel; Guráň, Roman; Kulich, Pavel; Kopel, Pavel; Masařík, Michal; Eckschlager, Tomáš; Stiborová, Marie; Kizek, René; Adam, Vojtěch (Nature Publishing Group, 2016-09-20)
    Herein, we describe the preparation of liposomes with folate-targeting properties for the encapsulation of anti-sarcosine antibodies (antisarAbs@LIP) and sarcosine (sar@LIP). The competitive inhibitory effects of exogenously ...
  • Sarcosine degradation pathway is involved in the epigenetics of prostate cells 

    Strmiska, Vladislav; Michálek, Petr; Buchtelová, Hana; Lacková, Zuzana; Guráň, Roman; Křížková, Soňa; Pompeiano Vaníčková, Lucie; Zítka, Ondřej; Stiborová, Marie; Eckschlager, Tomáš; Adam, Vojtěch; Heger, Zbyněk (Mendel University in Brno, 2017-12-31)
    It has been shown that sarcosine suplementation stimulates the proliferation of prostate cells and also their invassiveness. In present study we show that enzymes conected with sarcosine conversion to glycine (sarcosine ...
  • Sarcosine is a prostate epigenetic modifier that elicits aberrant methylation patterns through the SAMe-Dnmts axis 

    Strmiska, Vladislav; Michálek, Petr; Lacková, Zuzana; Guráň, Roman; Křížková, Soňa; Pompeiano Vaníčková, Lucie; Zítka, Ondřej; Stiborová, Marie; Eckschlager, Tomáš; Klejdus, Bořivoj; Pacík, Dalibor; Tvrdíková, Eliška; Keil, Claudia; Haase, Hajo; Adam, Vojtěch; Heger, Zbyněk (FEBS Press, 2019-05-31)
    DNA hypermethylation is one of the most common epigenetic modifications in prostate cancer (PCa). Several studies have delineated sarcosine as a PCa oncometabolite that increases the migration of malignant prostate cells ...
  • Staphylococcus aureus and MRSA Growth and Biofilm Formation after Treatment with Antibiotics and SeNPs 

    Číhalová, Kristýna; chudobova, Dagmar; Kizek, René; Michálek, Petr; Moulick, Amitava; Guráň, Roman; Kopel, Pavel; Adam, Vojtěch (MDPI, 2015-10-16)
    Methicillin-resistant Staphylococcus aureus (MRSA) is a dangerous pathogen resistant to -lactam antibiotics. Due to its resistance, it is difficult to manage the infections caused by this strain. We examined this issue in ...
  • Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients Swab 

    Komínková, Markéta; Michálek, Petr; Číhalová, Kristýna; Guráň, Roman; Cernei, Natalia Vladimirovna; Nejdl, Lukáš; Šmerková, Kristýna; Dostálová, Simona; Hegerová, Dagmar; Heger, Zbyněk; Veselý, Radek; Gumulec, Jaromír; Kynický, Jindřich; Xhaxhiu, Kledi; Zítka, Ondřej; Adam, Vojtěch; Kizek, René (MDPI, 2015-03-31)
    In this work, we focused on the differences between bacterial cultures of E. coli obtained from swabs of infectious wounds of patients compared to laboratory E. coli. In addition, blocking of the protein responsible for ...
  • Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients’ Swabs 

    Komínková, Markéta; Kizek, René; Michálek, Petr; Číhalová, Kristýna; Guráň, Roman; Cernei, Natalia Vladimirovna; Nejdl, Lukáš; Šmerková, Kristýna; Dostálová, Simona; chudobova, Dagmar; Heger, Zbyněk; Veselý, Radek; Gumulec, Jaromír; Kynický, Jindřich; Xhaxhiu, Kledi; Zítka, Ondřej; Adam, Vojtěch (MDPI, 2015-05-31)
    In this work, we focused on the differences between bacterial cultures of E. coli obtained from swabs of infectious wounds of patients compared to laboratory E. coli. In addition, blocking of the protein responsible for ...