Now showing items 1-20 of 25

  • Apoferritin as an ubiquitous nanocarrier with excellent shelf life 

    Dostálová, Simona; Vašíčková, Kateřina; Hynek, David; Křížková, Soňa; Richtera, Lukáš; Vaculovičová, Markéta; Eckschlager, Tomáš; Stiborová, Marie; Heger, Zbyněk; Adam, Vojtěch (Dove Media Press, 2017-03-24)
    Due to many adverse effects of conventional chemotherapy, novel methods of targeting drugs to cancer cells are being investigated. Nanosize carriers are a suitable platform for this specific delivery. Herein, we evaluated ...
  • Apoferritin-mediated doxorubicin internalization through transferrin receptor 1 

    Krausová, Kateřina; Dostálová, Simona; Hynek, David; Křížková, Soňa; Adam, Vojtěch; Heger, Zbyněk (Mendel University in Brno, 2017-12-31)
    This work is aimed at the possibilities of targeted drug delivery into the tumour tissue. This approach can greatly reduce the otherwise serious side effects of conventional treatment - systemic toxicity. For this purpose, ...
  • The application of capillary electrophoresis, mass spectrometry and Brdicka reaction in human and rabbit metallothioneins analysis 

    Kepinska, Marta; Křížková, Soňa; Guszpit, Ewelina; Merlos Rodrigo, Miguel Ángel; Milnerowicz, Halina (Wroclaw Medical University, 2018-11-30)
    Background. Metallothioneins (MTs) constitute a family of evolutionary conserved low molecular weight proteins with small variations in their amino acid sequences. They play a role in the regulation of trace metals metabolism, ...
  • Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots 

    Krejčová, Ludmila; Nejdl, Lukáš; Hynek, David; Křížková, Soňa; Kopel, Pavel; Adam, Vojtěch; Kizek, René (MDPI, 2013-12-13)
    In this study we describe a beads-based assay for rapid, sensitive and specific isolation and detection of influenza vaccine hemagglutinin (HA). Amplification of the hemagglutinin signal resulted from binding of an ...
  • Comparative gene expression profiling of human metallothionein-3 up-regulation in neuroblastoma cells and its impact on susceptibility to cisplatin 

    Merlos Rodrigo, Miguel Ángel; Dostálová, Simona; Buchtelová, Hana; Strmiska, Vladislav; Michálek, Petr; Křížková, Soňa; Vícha, Aleš; Eckschlager, Tomáš; Stiborová, Marie; Heger, Zbyněk; Adam, Vojtěch (Impact Journals, 2018-01-12)
    Human metallothionein-3 (hMT-3), also known as growth inhibitory factor, is predominantly expressed in the central nervous system. hMT-3 is presumed to participate in the processes of heavy metal detoxification, regulation ...
  • Evaluation of cytotoxicity of biphasic TiO2 nanoparticles with organic surface coatings 

    Škubalová, Zuzana; Buchtelová, Hana; Strmiska, Vladislav; Dostálová, Simona; Michálek, Petr; Křížková, Soňa; Adam, Vojtěch; Heger, Zbyněk (Mendel University in Brno, 2019-01-02)
    Titanium dioxide nanoparticles (TiO2 NPs) are used in lots of human applications because of their extraordinary nano scaled properties. Particularly, due to their photoprotective properties, they are used in topical ...
  • Flow Injection Analysis Coupled with Carbon Electrodes as the Tool for Analysis of Naphthoquinones with Respect to Their Content and Functions in Biological Samples 

    Babula, Petr; Křížková, Soňa; Adam, Vojtěch; Hubálek, Jaromír; Kizek, René (MDPI, 2006-10-01)
    Naphthoquinones are one of the groups of secondary metabolites widespread in nature, where they mostly appear as chromatic pigments. They embody broad-range of biological actions from phytotoxic to fungicidal. An anticancer ...
  • Fluorescence-tagged metallothionein with CdTe quantum dots analyzed by the chip-CE technique 

    Guszpit, Ewelina; Křížková, Soňa; Kepinska, Marta; Merlos Rodrigo, Miguel Ángel; Milnerowitz, Halina; Kopel, Pavel; Kizek, René (Springer, 2015-10-28)
    Quantum dots (QDs) are fluorescence nanoparticles (NPs) with unique optic properties which allow their use as probes in chemical, biological, immunological, and molecular imaging. QDs linked with target ligands such as ...
  • hNET as a target for neuroblastoma nanomedicine 

    Charousová, Markéta; Dostálová, Simona; Haddad, Yazan Abdulmajeed Eyadh; Strmiska, Vladislav; Křížková, Soňa; Hynek, David; Milosavljević, Vedran; Adam, Vojtěch; Heger, Zbyněk (Mendel University in Brno, 2017-12-31)
    Chemotherapy often results in various side effects, which can negatively affect health. Neuroblastoma, one of the most common types of childhood cancer, is but one of the examples, where side effects of chemotherapeutic ...
  • Improving cytocompatibility of CdTe quantum dots by Schiff-base-coordinated lanthanides surface doping 

    Buchtelová, Hana; Strmiska, Vladislav; Škubalová, Zuzana; Dostálová, Simona; Michálek, Petr; Křížková, Soňa; Hynek, David; Kalina, Lukáš; Richtera, Lukáš; Moulick, Amitava; Adam, Vojtěch; Heger, Zbyněk (BioMed Central, 2018-04-19)
    Background: Suitable fluorophores are the core of fluorescence imaging. Among the most exciting, yet controversial, labels are quantum dots (QDs) with their unique optical and chemical properties, but also considerable ...
  • Investigating the interplay between sarcosine and Ca2+-dependent signaling in prostate cells 

    Strmiska, Vladislav; Buchtelová, Hana; Michálek, Petr; Křížková, Soňa; Adam, Vojtěch; Heger, Zbyněk (Mendel University in Brno, 2019-01-02)
    It has been shown that sarcosine supplementation stimulates proliferation and invassivenes of prostate cells. Nevertheless, the exact molecular mechanism responsible for this phenomenon is not known. In the present study ...
  • An Investigation of Glutathione-Platinum(II) Interactions by Means of the Flow Injection Analysis Using Glassy Carbon Electrode 

    Zítka, Ondřej; Křížková, Soňa; Adam, Vojtěch; Trnková, Libuše; Hubálek, Jaromír; Kizek, René (MDPI, 2007-07-20)
    Glutathione was discovered by M. J. de Rey Pailhade at the end of the 19th century as the substance (hydrogénant le soufre), which was renamed by F.G. Hopkins in 1921. Hopkins first characterised the compound as a dipeptide ...
  • Lactoferrin Isolation Using Monolithic Column Coupled with Spectrometric or Micro-Amperometric Detector 

    Adam, Vojtěch; Zítka, Ondřej; Hubálek, Jaromír; Křížková, Soňa; Trnková, Libuše; Kizek, René (MDPI, 2008-01-24)
    Lactoferrin is a multifunctional protein with antimicrobial activity and others to health beneficial properties. The main aim of this work was to propose easy to use technique for lactoferrin isolation from cow colostrum ...
  • MALDI MSI of MeLiM melanoma: Searching for differences in protein profiles 

    Guráň, Roman; Pompeiano Vaníčková, Lucie; Horák, Vratislav; Křížková, Soňa; Michálek, Petr; Heger, Zbyněk; Zítka, Ondřej; Adam, Vojtěch (PLOS, 2017-12-08)
    Background Treatment of advanced cutaneous melanoma remains challenging, and new data on melanoma biology are required. The most widely accepted criteria for the prognostic evaluation of melanoma are histopathological and ...
  • Mass spectrometric imaging of cysteine rich proteins in human skin 

    Pompeiano Vaníčková, Lucie; Guráň, Roman; Kollar, Sandor; Emri, Gabriela; Křížková, Soňa; Do, Tomáš; Heger, Zbyněk; Zítka, Ondřej; Adam, Vojtěch (Elsevier, 2019-03-15)
    Looking insight pathological processes, metallothioneins (MTs) are considered to be potential biomarkers for monitoring of a development of various types of diseases, such as cancer. The early identification of the MTs in ...
  • Metallothionein Isoforms as Double Agents - Their Roles in Carcinogenesis, Cancer Progression and Chemoresistance 

    Merlos Rodrigo, Miguel Ángel; Jimenez Jimenez, Ana Maria; Haddad, Yazan Abdulmajeed Eyadh; Adam, Pavlína; Křížková, Soňa; Heger, Zbyněk; Adam, Vojtěch (Elsevier, 2020-09-30)
    Metallothioneins (MTs) are small cysteine-rich intracellular proteins with four major isoforms identified in mammals, designated MT-1 through MT-4. The best known biological functions of MTs are their ability to bind and ...
  • Multi-instrumental Analysis of Tissues of Sunflower Plants Treated with Silver(I) Ions - Plants as Bioindicators of Environmental Pollution 

    Křížková, Soňa; Kryštofová, Olga; Adam, Vojtěch; Vašinová Galiová, Michaela; Beklová, Miroslava; Babula, Petr; Kaiser, Jozef; Liška, Miroslav; Malina, Radomír; Zehnálek, Josef; Hubálek, Jaromír; Kizek, René (MDPI, 2008-01-24)
    The aim of this work is to investigate sunflower plants response on stress induced by silver(I) ions. The sunflower plants were exposed to silver(I) ions (0, 0.1, 0.5, and 1 mM) for 96 h. Primarily we aimed our attention ...
  • Norepinephrine Transporter-Derived Homing Peptides Enable Rapid Endocytosis of Drug Delivery Nanovehicles into Neuroblastoma Cells 

    Haddad, Yazan Abdulmajeed Eyadh; Charousová, Markéta; Životská, Hana; Šplíchal, Zbyněk; Merlos Rodrigo, Miguel Ángel; Michálková, Hana; Křížková, Soňa; Tesařová, Barbora; Richtera, Lukáš; Vítek, Petr; Stokowa-Soltys, Kamila; Hynek, David; Milosavljević, Vedran; Rex, Simona; Heger, Zbyněk (BioMed Central, 2020-07-13)
    Background: Currently, the diagnosis and treatment of neuroblastomas-the most frequent solid tumors in children-exploit the norepinephrine transporter (hNET) via radiolabeled norepinephrine analogs. We aim to develop a ...
  • Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection 

    Heger, Zbyněk; Cernei, Natalia Vladimirovna; Křížková, Soňa; Masařík, Michal; Kopel, Pavel; Hodek, Petr; Zítka, Ondřej; Adam, Vojtěch; Kizek, René (Nature Publishing Group, 2015-03-09)
    Herein, we describe an ultrasensitive specific biosensing system for detection of sarcosine as a potential biomarker of prostate carcinoma based on Forster resonance energy transfer (FRET). The FRET biosensor employs ...
  • Platinum nanoparticles induce damage to DNA and inhibit DNA replication 

    Nejdl, Lukáš; Kudr, Jiří; Moulick, Amitava; Hegerová, Dagmar; Ruttkay-Nedecký, Branislav; Gumulec, Jaromír; Číhalová, Kristýna; Šmerková, Kristýna; Dostálová, Simona; Křížková, Soňa; Novotná, Marie; Kopel, Pavel; Adam, Vojtěch (PLOS, 2017-07-12)
    Sparsely tested group of platinum nanoparticles (PtNPs) may have a comparable effect as complex platinum compounds. The aim of this study was to observe the effect of PtNPs in in vitro amplification of DNA fragment of phage ...