Now showing items 1-20 of 21

  • Apoferritin as an ubiquitous nanocarrier with excellent shelf life 

    Dostálová, Simona; Vašíčková, Kateřina; Hynek, David; Křížková, Soňa; Richtera, Lukáš; Vaculovičová, Markéta; Eckschlager, Tomáš; Stiborová, Marie; Heger, Zbyněk; Adam, Vojtěch (Dove Media Press, 2017-03-24)
    Due to many adverse effects of conventional chemotherapy, novel methods of targeting drugs to cancer cells are being investigated. Nanosize carriers are a suitable platform for this specific delivery. Herein, we evaluated ...
  • Apoferritin-mediated doxorubicin internalization through transferrin receptor 1 

    Krausová, Kateřina; Dostálová, Simona; Hynek, David; Křížková, Soňa; Adam, Vojtěch; Heger, Zbyněk (Mendel University in Brno, 2017-12-31)
    This work is aimed at the possibilities of targeted drug delivery into the tumour tissue. This approach can greatly reduce the otherwise serious side effects of conventional treatment - systemic toxicity. For this purpose, ...
  • The application of capillary electrophoresis, mass spectrometry and Brdicka reaction in human and rabbit metallothioneins analysis 

    Kepinska, Marta; Křížková, Soňa; Guszpit, Ewelina; Merlos Rodrigo, Miguel Ángel; Milnerowicz, Halina (Wroclaw Medical University, 2018-11-30)
    Background. Metallothioneins (MTs) constitute a family of evolutionary conserved low molecular weight proteins with small variations in their amino acid sequences. They play a role in the regulation of trace metals metabolism, ...
  • Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots 

    Krejčová, Ludmila; Nejdl, Lukáš; Hynek, David; Křížková, Soňa; Kopel, Pavel; Adam, Vojtěch; Kizek, René (MDPI, 2013-12-13)
    In this study we describe a beads-based assay for rapid, sensitive and specific isolation and detection of influenza vaccine hemagglutinin (HA). Amplification of the hemagglutinin signal resulted from binding of an ...
  • Comparative gene expression profiling of human metallothionein-3 up-regulation in neuroblastoma cells and its impact on susceptibility to cisplatin 

    Merlos Rodrigo, Miguel Ángel; Dostálová, Simona; Buchtelová, Hana; Strmiska, Vladislav; Michálek, Petr; Křížková, Soňa; Vícha, Aleš; Eckschlager, Tomáš; Stiborová, Marie; Heger, Zbyněk; Adam, Vojtěch (Impact Journals, 2018-01-12)
    Human metallothionein-3 (hMT-3), also known as growth inhibitory factor, is predominantly expressed in the central nervous system. hMT-3 is presumed to participate in the processes of heavy metal detoxification, regulation ...
  • Evaluation of cytotoxicity of biphasic TiO2 nanoparticles with organic surface coatings 

    Škubalová, Zuzana; Buchtelová, Hana; Strmiska, Vladislav; Dostálová, Simona; Michálek, Petr; Křížková, Soňa; Adam, Vojtěch; Heger, Zbyněk (Mendel University in Brno, 2019-01-02)
    Titanium dioxide nanoparticles (TiO2 NPs) are used in lots of human applications because of their extraordinary nano scaled properties. Particularly, due to their photoprotective properties, they are used in topical ...
  • Flow Injection Analysis Coupled with Carbon Electrodes as the Tool for Analysis of Naphthoquinones with Respect to Their Content and Functions in Biological Samples 

    Babula, Petr; Křížková, Soňa; Adam, Vojtěch; Hubálek, Jaromír; Kizek, René (MDPI, 2006-10-01)
    Naphthoquinones are one of the groups of secondary metabolites widespread in nature, where they mostly appear as chromatic pigments. They embody broad-range of biological actions from phytotoxic to fungicidal. An anticancer ...
  • hNET as a target for neuroblastoma nanomedicine 

    Charousová, Markéta; Dostálová, Simona; Haddad, Yazan Abdulmajeed Eyadh; Strmiska, Vladislav; Křížková, Soňa; Hynek, David; Milosavljević, Vedran; Adam, Vojtěch; Heger, Zbyněk (Mendel University in Brno, 2017-12-31)
    Chemotherapy often results in various side effects, which can negatively affect health. Neuroblastoma, one of the most common types of childhood cancer, is but one of the examples, where side effects of chemotherapeutic ...
  • Improving cytocompatibility of CdTe quantum dots by Schiff-base-coordinated lanthanides surface doping 

    Buchtelová, Hana; Strmiska, Vladislav; Škubalová, Zuzana; Dostálová, Simona; Michálek, Petr; Křížková, Soňa; Hynek, David; Kalina, Lukáš; Richtera, Lukáš; Moulick, Amitava; Adam, Vojtěch; Heger, Zbyněk (BioMed Central, 2018-04-19)
    Background: Suitable fluorophores are the core of fluorescence imaging. Among the most exciting, yet controversial, labels are quantum dots (QDs) with their unique optical and chemical properties, but also considerable ...
  • Investigating the interplay between sarcosine and Ca2+-dependent signaling in prostate cells 

    Strmiska, Vladislav; Buchtelová, Hana; Michálek, Petr; Křížková, Soňa; Adam, Vojtěch; Heger, Zbyněk (Mendel University in Brno, 2019-01-02)
    It has been shown that sarcosine supplementation stimulates proliferation and invassivenes of prostate cells. Nevertheless, the exact molecular mechanism responsible for this phenomenon is not known. In the present study ...
  • An Investigation of Glutathione-Platinum(II) Interactions by Means of the Flow Injection Analysis Using Glassy Carbon Electrode 

    Zítka, Ondřej; Křížková, Soňa; Adam, Vojtěch; Trnková, Libuše; Hubálek, Jaromír; Kizek, René (MDPI, 2007-07-20)
    Glutathione was discovered by M. J. de Rey Pailhade at the end of the 19th century as the substance (hydrogénant le soufre), which was renamed by F.G. Hopkins in 1921. Hopkins first characterised the compound as a dipeptide ...
  • Lactoferrin Isolation Using Monolithic Column Coupled with Spectrometric or Micro-Amperometric Detector 

    Adam, Vojtěch; Zítka, Ondřej; Hubálek, Jaromír; Křížková, Soňa; Trnková, Libuše; Kizek, René (MDPI, 2008-01-24)
    Lactoferrin is a multifunctional protein with antimicrobial activity and others to health beneficial properties. The main aim of this work was to propose easy to use technique for lactoferrin isolation from cow colostrum ...
  • MALDI MSI of MeLiM melanoma: Searching for differences in protein profiles 

    Guráň, Roman; Pompeiano Vaníčková, Lucie; Horák, Vratislav; Křížková, Soňa; Michálek, Petr; Heger, Zbyněk; Zítka, Ondřej; Adam, Vojtěch (PLOS, 2017-12-08)
    Background Treatment of advanced cutaneous melanoma remains challenging, and new data on melanoma biology are required. The most widely accepted criteria for the prognostic evaluation of melanoma are histopathological and ...
  • Multi-instrumental Analysis of Tissues of Sunflower Plants Treated with Silver(I) Ions - Plants as Bioindicators of Environmental Pollution 

    Křížková, Soňa; Kryštofová, Olga; Adam, Vojtěch; Vašinová Galiová, Michaela; Beklová, Miroslava; Babula, Petr; Kaiser, Jozef; Liška, Miroslav; Malina, Radomír; Zehnálek, Josef; Hubálek, Jaromír; Kizek, René (MDPI, 2008-01-24)
    The aim of this work is to investigate sunflower plants response on stress induced by silver(I) ions. The sunflower plants were exposed to silver(I) ions (0, 0.1, 0.5, and 1 mM) for 96 h. Primarily we aimed our attention ...
  • Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection 

    Heger, Zbyněk; Cernei, Natalia Vladimirovna; Křížková, Soňa; Masařík, Michal; Kopel, Pavel; Hodek, Petr; Zítka, Ondřej; Adam, Vojtěch; Kizek, René (Nature Publishing Group, 2015-03-09)
    Herein, we describe an ultrasensitive specific biosensing system for detection of sarcosine as a potential biomarker of prostate carcinoma based on Forster resonance energy transfer (FRET). The FRET biosensor employs ...
  • Platinum nanoparticles induce damage to DNA and inhibit DNA replication 

    Nejdl, Lukáš; Kudr, Jiří; Moulick, Amitava; Hegerová, Dagmar; Ruttkay-Nedecký, Branislav; Gumulec, Jaromír; Číhalová, Kristýna; Šmerková, Kristýna; Dostálová, Simona; Křížková, Soňa; Novotná, Marie; Kopel, Pavel; Adam, Vojtěch (PLOS, 2017-07-12)
    Sparsely tested group of platinum nanoparticles (PtNPs) may have a comparable effect as complex platinum compounds. The aim of this study was to observe the effect of PtNPs in in vitro amplification of DNA fragment of phage ...
  • Prostate-Specific Membrane Antigen-Targeted Site-Directed Antibody-Conjugated Apoferritin Nanovehicle Favorably Influences In Vivo Side Effects of Doxorubicin 

    Dostálová, Simona; Polanská, Hana; Svobodová, Markéta; Balvan, Jan; Kryštofová, Olga; Haddad, Yazan Abdulmajeed Eyadh; Křížková, Soňa; Masařík, Michal; Eckschlager, Tomáš; Stiborová, Marie; Heger, Zbyněk; Adam, Vojtěch (Springer Nature, 2018-06-11)
    Herein, we describe the in vivo effects of doxorubicin (DOX) encapsulated in ubiquitous protein apoferritin (APO) and its efficiency and safety in anti-tumor treatment. APODOX is both passively (through Enhanced Permeability ...
  • Ruthenium-based core-shell nanoparticles with exceptional in vitro biocompatibility 

    Buchtelová, Hana; Strmiska, Vladislav; Dostálová, Simona; Michálek, Petr; Křížková, Soňa; Kopel, Pavel; Hynek, David; Richtera, Lukáš; Adam, Vojtěch; Heger, Zbyněk (Mendel University in Brno, 2017-12-31)
    The current study demonstrates design preparation and characterization of biocompatible hybrid ruthenium core-shell nanoparticles (RuNPs) coated with polyvinylpyrrolidone (PVP) and polyoxyethylene stearate (POES). The ...
  • Sarcosine degradation pathway is involved in the epigenetics of prostate cells 

    Strmiska, Vladislav; Michálek, Petr; Buchtelová, Hana; Lacková, Zuzana; Guráň, Roman; Křížková, Soňa; Pompeiano Vaníčková, Lucie; Zítka, Ondřej; Stiborová, Marie; Eckschlager, Tomáš; Adam, Vojtěch; Heger, Zbyněk (Mendel University in Brno, 2017-12-31)
    It has been shown that sarcosine suplementation stimulates the proliferation of prostate cells and also their invassiveness. In present study we show that enzymes conected with sarcosine conversion to glycine (sarcosine ...
  • Sarcosine is a prostate epigenetic modifier that elicits aberrant methylation patterns through the SAMe-Dnmts axis 

    Strmiska, Vladislav; Michálek, Petr; Lacková, Zuzana; Guráň, Roman; Křížková, Soňa; Pompeiano Vaníčková, Lucie; Zítka, Ondřej; Stiborová, Marie; Eckschlager, Tomáš; Klejdus, Bořivoj; Pacík, Dalibor; Tvrdíková, Eliška; Keil, Claudia; Haase, Hajo; Adam, Vojtěch; Heger, Zbyněk (FEBS Press, 2019-05-31)
    DNA hypermethylation is one of the most common epigenetic modifications in prostate cancer (PCa). Several studies have delineated sarcosine as a PCa oncometabolite that increases the migration of malignant prostate cells ...