Now showing items 1-20 of 69

  • A 3D Microfluidic Chip for Electrochemical Detection of Hydrolysed Nucleic Bases by a Modified Glassy Carbon Electrode 

    Vlachová, Jana; Tmejová, Kateřina; Kopel, Pavel; Korabík, Michal; Zítka, Jan; Hynek, David; Kynický, Jindřich; Adam, Vojtěch; Kizek, René (MDPI AG, POSTFACH, CH-4005 BASEL, SWITZERLAND, 2015-02-01)
    Modification of carbon materials, especially graphene-based materials, has wide applications in electrochemical detection such as electrochemical lab-on-chip devices. A glassy carbon electrode (GCE) modified with chemically ...
  • An Acetylcholinesterase-Based Chronoamperometric Biosensor for Fast and Reliable Assay of Nerve Agents 

    Pohanka, Miroslav; Adam, Vojtěch; Kizek, René (MDPI, 2013-09-01)
    The enzyme acetylcholinesterase (AChE) is an important part of cholinergic nervous system, where it stops neurotransmission by hydrolysis of the neurotransmitter acetylcholine. It is sensitive to inhibition by organophosphate ...
  • Algal Biomass Analysis by Laser-Based Analytical Techniques - A Review 

    Pořízka, Pavel; Prochazková, Petra; Prochazka, David; Sládková, Lucia; Novotný, Jan; Petrilak, Michal; Brada, Michal; Samek, Ota; Pilát, Zdeněk; Zemánek, Pavel; Adam, Vojtěch; Kizek, René; Novotný, Karel; Kaiser, Jozef (MDPI AG, 2014-09-23)
    Algal biomass that is represented mainly by commercially grown algal strains has recently found many potential applications in various fields of interest. Its utilization has been found advantageous in the fields of ...
  • Amperometric Sensor for Detection of Chloride Ions 

    Trnková, Libuše; Adam, Vojtěch; Hubálek, Jaromír; Babula, Petr; Kizek, René (MDPI, 2008-09-15)
    Chloride ion sensing is important in many fields such as clinical diagnosis, environmental monitoring and industrial applications. We have measured chloride ions at a carbon paste electrode (CPE) and at a CPE modified with ...
  • Analýza signalů tlustovrstvých ampérometrických senzorů a jejich použití pro měření a charakterizaci enzymů 

    Ondruch, Vít
    V práci je popsán princip synchronní detekce (SD), který byl uplatněn při měření s biosenzory. Metoda SD umožňuje dosažení výrazně lepšího poměru signálu k šumu, vyššího limitu detekce a celkové zlepšení robustnosti měření. ...
  • Apoferritin modified magnetic particles as doxorubicin carriers for anticancer drug delivery 

    Blažková, Iva; Nguyen, Hoai Viet; Dostálová, Simona; Kopel, Pavel; Stanisavljevic, Maja; Vaculovičová, Markéta; Stiborová, Marie; Eckschlager, Tomáš; Kizek, René; Adam, Vojtěch (MDPI, 2013-06-27)
    Magnetic particle mediated transport in combination with nanomaterial based drug carrier has a great potential for targeted cancer therapy. In this study, doxorubicin encapsulation into the apoferritin and its conjugation ...
  • Are Early Somatic Embryos of the Norway Spruce (Picea abies (L.) Karst.) Organised? 

    Petřek, Jiří; Zítka, Ondřej; Adam, Vojtěch; Bartušek, Karel; Anjum, Naser A.; Pereira, Eduarda; Havel, Ladislav; Kizek, René (PLOS, 2015-12-01)
    Somatic embryogenesis in conifer species has great potential for the forestry industry. Hence, a number of methods have been developed for their efficient and rapid propagation through somatic embryogenesis. Although ...
  • Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots 

    Krejčová, Ludmila; Nejdl, Lukáš; Hynek, David; Křížková, Soňa; Kopel, Pavel; Adam, Vojtěch; Kizek, René (MDPI, 2013-12-13)
    In this study we describe a beads-based assay for rapid, sensitive and specific isolation and detection of influenza vaccine hemagglutinin (HA). Amplification of the hemagglutinin signal resulted from binding of an ...
  • Behaviour of zinc complexes and zinc sulphide nanoparticles revealed by using screen printed electrodes and spectrometry 

    Nejdl, Lukáš; Ruttkay-Nedecký, Branislav; Kudr, Jiří; Kremplová, Monika; Cernei, Natalia Vladimirovna; Prášek, Jan; Konečná, Marie; Hubálek, Jaromír; Zítka, Ondřej; Kynický, Jindřich; Kopel, Pavel; Kizek, René; Adam, Vojtěch (MDPI AQ, 2013-10-25)
    In this study, we focused on microfluidic electrochemical analysis of zinc complexes (Zn(phen)(his)Cl-2, Zn(his)Cl-2) and ZnS quantum dots (QDs) using printed electrodes. This method was chosen due to the simple (easy to ...
  • Bio-Sensing of Cadmium(II) Ions Using Staphylococcus aureus 

    Sochor, Jiří; Zítka, Ondřej; Hynek, David; Krejčová, Ludmila; Trnková, Libuše; Adam, Vojtěch; Hubálek, Jaromír; Kynický, Jindřich; Vrba, Radimír; Kizek, René (MDPI, 2011-11-08)
    Cadmium, as a hazardous pollutant commonly present in the living environment, represents an important risk to human health due to its undesirable effects (oxidative stress, changes in activities of many enzymes, interactions ...
  • Biological Activity and Molecular Structures of Bis(benzimidazole) and Trithiocyanurate Complexes 

    Kopel, Pavel; Wawrzak, Dorota; Langer, Vratislav; Číhalová, Kristýna; Hegerová, Dagmar; Veselý, Radek; Adam, Vojtěch; Kizek, René (MDPI, 2015-06-01)
    1-(1H-Benzimidazol-2-yl)-N-(1H-benzimidazol-2-ylmethyl)methanamine (abb) and 2-(1H-benzimidazol-2-ylmethylsulfanylmethyl)-1H-benzimidazole (tbb) have been prepared and characterized by elemental analysis. These bis(benzimidazoles) ...
  • Carbon composite micro- and nano-tubes-based electrodes for detection of nucleic acids 

    Prášek, Jan; Húska, Dalibor; Trnková, Libuše; Adam, Vojtěch; Kizek, René; Hubálek, Jaromír (Springer, 2011-05-16)
    The first aim of this study was to fabricate vertically aligned multiwalled carbon nanotubes (MWCNTs). MWCNTs were successfully prepared by using plasma enhanced chemical vapour deposition. Further, three carbon composite ...
  • Complexes of silver(I) ions and silver phosphate nanoparticles with hyaluronic acid and/or chitosan as promising antimicrobial agents for vascular grafts 

    Hegerová, Dagmar; Nejdl, Lukáš; Gumulec, Jaromír; Kryštofová, Olga; Merlos Rodrigo, Miguel Ángel; Kynický, Jindřich; Ruttkay-Nedecký, Branislav; Kopel, Pavel; Babula, Petr; Adam, Vojtěch; Kizek, René (MDPI, 2013-06-28)
    Polymers are currently widely used to replace a variety of natural materials with respect to their favourable physical and chemical properties, and due to their economic advantage. One of the most important branches of ...
  • The Composites of Graphene Oxide with Metal or Semimetal Nanoparticles and Their Effect on Pathogenic Microorganisms 

    Richtera, Lukáš; Hegerová, Dagmar; Číhalová, Kristýna; Kremplová, Monika; Milosavljević, Vedran; Kopel, Pavel; Blažková, Iva; Hynek, David; Adam, Vojtěch; Kizek, René (MDPI, 2015-06-01)
    The present experiment describes a synthesis process of composites based on graphene oxide, which was tested as a carrier for composites of metal- or metalloid-based nanoparticles (Cu, Zn, Mn, Ag, AgP, Se) and subsequently ...
  • Content of phenolic compounds and antioxidant capacity in fruits of selected genotypes of apricot with resistance against Plum pox virus 

    Sochor, Jiří; Zítka, Ondřej; Škutková, Helena; Pavlík, Dušan; Babula, Petr; Krška, Boris; Horna, Aleš; Adam, Vojtěch; Provazník, Ivo; Kizek, René (MDPI, 2010-09-07)
    Research on natural compounds is increasingly focused on their effects on human health. In this study, we were interested in the evaluation of nutritional value expressed as content of total phenolic compounds and antioxidant ...
  • Development of a magnetic electrochemical bar code array for point mutation detection in the H5N1 neuraminidase gene 

    Krejčová, Ludmila; Hynek, David; Kopel, Pavel; Merlos Rodrigo, Miguel Ángel; Adam, Vojtěch; Hubálek, Jaromír; Babula, Petr; Trnková, Libuše; Kizek, René (MDPI, 2013-07-15)
    Since its first official detection in the Guangdong province of China in 1996, the highly pathogenic avian influenza virus of H5N1 subtype (HPAI H5N1) has reportedly been the cause of outbreaks in birds in more than 60 ...
  • Effect of Ampicillin, Streptomycin, Penicillin and Tetracycline on Metal Resistant and Non-Resistant Staphylococcus aureus 

    Hegerová, Dagmar; Dostálová, Simona; Blažková, Iva; Michálek, Petr; Ruttkay-Nedecký, Branislav; Sklenář, Matěj; Nejdl, Lukáš; Kudr, Jiří; Gumulec, Jaromír; Tmejová, Kateřina; Konečná, Marie; Vaculovičová, Markéta; Hynek, David; Masařík, Michal; Kynický, Jindřich; Kizek, René; Adam, Vojtěch (MDPI AG, POSTFACH, 2014-03-19)
    There is an arising and concerning issue in the field of bacterial resistance, which is confirmed by the number of deaths associated with drug-resistant bacterial infections. The aim of this study was to compare the effects ...
  • Effect of five different stages of ripening on chemical compounds in medlar (Mespilus germanica L.) 

    Rop, Otakar; Sochor, Jiří; Juříková, Tunde; Zítka, Ondřej; Škutková, Helena; Mlček, Jiří; Salaš, Petr; Krška, Boris; Babula, Petr; Adam, Vojtěch; Kramářová, Daniela; Beklová, Miroslava; Provazník, Ivo; Kizek, René (MDPI, 2010-12-28)
    Study of changes of nutritional value of fruit during the process of ripening can help to estimate the optimal date for fruit harvesting to achieve the best quality for direct consumption and further utilization. The aim ...
  • Effect of Magnetic Nanoparticles on Tobacco BY-2 Cell Suspension Culture 

    Kryštofová, Olga; Sochor, Jiří; Zítka, Ondřej; Babula, Petr; Kudrle, Vít; Adam, Vojtěch; Kizek, René (MDPI, 2013-01-01)
    Nanomaterials are structures whose exceptionality is based on their large surface, which is closely connected with reactivity and modification possibilities. Due to these properties nanomaterials are used in textile industry ...
  • Effects of stratospheric conditions on viability, metabolism and proteome of prokaryotic cells 

    chudobova, Dagmar; Číhalová, Kristýna; Jelínková, Pavlína; Zítka, Jan; Nejdl, Lukáš; Guráň, Roman; Klimánek, Martin; Adam, Vojtěch; Kizek, René (MDPI, 2015-09-09)
    The application of ultraviolet (UV) radiation to inhibit bacterial growth is based on the principle that the exposure of DNA to UV radiation results in the formation of cytotoxic lesions, leading to inactivation of ...