Now showing items 1-4 of 4

  • Handheld Real-Time PCR Device 

    Ahrberg, Christian D.; Ilic, Bojan Robert; Manz, Andreas; Neužil, Pavel (Royal Society of Chemistry, 2016-01-04)
    Here we report one of the smallest real-time polymerase chain reaction (PCR) system up to date with approximate size of 100 mm × 60 mm × 33 mm. The system is an autonomous unit requiring an external 12 V power supply. Four ...
  • Nanoliter-sized overheated reactor 

    Neužil, Pavel; Sun, Wanxin; Karásek, Tomáš; Manz, Andreas (AIP Publishing, 2015-01-16)
    We report on a microfluidic system formed by 200 nl water droplets, encapsulated by a 600 nl mineral oil placed on a hydrophobically coated glass microscope cover slip. The micromachined heater underneath the glass was ...
  • On-chip three-dimensional cell culture in phaseguides improves hepatocyte functions in vitro 

    Jang, Mi; Neužil, Pavel; Volk, Thomas; Manz, Andreas; Kleber, Astrid (AIP Publishing, 2015-06-23)
    The in vitro study of liver functions and liver cell specific responses to external stimuli deals with the problem to preserve the in vivo functions of primary hepatocytes. In this study, we used the biochip OrganoPlateTM ...
  • Single Fluorescence Channel-based Multiplex Detection of Avian Influenza Virus by Quantitative PCR with Intercalating Dye 

    Ahrberg, Christian D.; Manz, Andreas; Neužil, Pavel (Nature Publishing Group, 2015-06-19)
    Since its invention in 1985 the polymerase chain reaction (PCR) has become a well-established method for amplification and detection of segments of double-stranded DNA. Incorporation of fluorogenic probe or DNA intercalating ...