Now showing items 1-19 of 19

  • Comparative gene expression profiling of human metallothionein-3 up-regulation in neuroblastoma cells and its impact on susceptibility to cisplatin 

    Merlos Rodrigo, Miguel Ángel; Dostálová, Simona; Buchtelová, Hana; Strmiska, Vladislav; Michálek, Petr; Křížková, Soňa; Vícha, Aleš; Eckschlager, Tomáš; Stiborová, Marie; Heger, Zbyněk; Adam, Vojtěch (Impact Journals, 2018-01-12)
    Human metallothionein-3 (hMT-3), also known as growth inhibitory factor, is predominantly expressed in the central nervous system. hMT-3 is presumed to participate in the processes of heavy metal detoxification, regulation ...
  • Comparison of dietary oils with different polyunsaturated fatty acid n-3 and n-6 content in the rat model of cutaneous wound healing 

    Komprda, Tomáš; Sládek, Zbyšek; Ševčíková, Zuzana; Švehlová, Veronika; Wijacki, Jan; Guráň, Roman; Do, Tomáš; Lacková, Zuzana; Polanská, Hana; Vrlíková, Lucie; Popelková, Vendula; Michálek, Petr; Zítka, Ondřej; Buchtová, Marcela (MDPI, 2020-10-24)
    Dietary supplementation with polyunsaturated fatty acids (PUFA) n-3 can affect cutaneous wound healing; however, recent findings demonstrate the variable extent of their influence on the quality of healing. Here, we compare ...
  • Effect of Ampicillin, Streptomycin, Penicillin and Tetracycline on Metal Resistant and Non-Resistant Staphylococcus aureus 

    Hegerová, Dagmar; Dostálová, Simona; Blažková, Iva; Michálek, Petr; Ruttkay-Nedecký, Branislav; Sklenář, Matěj; Nejdl, Lukáš; Kudr, Jiří; Gumulec, Jaromír; Tmejová, Kateřina; Konečná, Marie; Vaculovičová, Markéta; Hynek, David; Masařík, Michal; Kynický, Jindřich; Kizek, René; Adam, Vojtěch (MDPI AG, POSTFACH, 2014-03-19)
    There is an arising and concerning issue in the field of bacterial resistance, which is confirmed by the number of deaths associated with drug-resistant bacterial infections. The aim of this study was to compare the effects ...
  • Evaluation of cytotoxicity of biphasic TiO2 nanoparticles with organic surface coatings 

    Škubalová, Zuzana; Buchtelová, Hana; Strmiska, Vladislav; Dostálová, Simona; Michálek, Petr; Křížková, Soňa; Adam, Vojtěch; Heger, Zbyněk (Mendel University in Brno, 2019-01-02)
    Titanium dioxide nanoparticles (TiO2 NPs) are used in lots of human applications because of their extraordinary nano scaled properties. Particularly, due to their photoprotective properties, they are used in topical ...
  • Exposure to 17 beta-Oestradiol Induces Oxidative Stress in the Non-Oestrogen Receptor Invertebrate Species Eisenia fetida 

    Heger, Zbyněk; Michálek, Petr; Guráň, Roman; Havelková, Barbora; Komínková, Markéta; Cernei, Natalia Vladimirovna; Richtera, Lukáš; Beklová, Miroslava; Adam, Vojtěch; Kizek, René (PLOS, 2015-12-22)
    Background The environmental impacts of various substances on all levels of organisms are under investigation. Among these substances, endocrine-disrupting compounds (EDCs) present a threat, although the environmental ...
  • Improving cytocompatibility of CdTe quantum dots by Schiff-base-coordinated lanthanides surface doping 

    Buchtelová, Hana; Strmiska, Vladislav; Škubalová, Zuzana; Dostálová, Simona; Michálek, Petr; Křížková, Soňa; Hynek, David; Kalina, Lukáš; Richtera, Lukáš; Moulick, Amitava; Adam, Vojtěch; Heger, Zbyněk (BioMed Central, 2018-04-19)
    Background: Suitable fluorophores are the core of fluorescence imaging. Among the most exciting, yet controversial, labels are quantum dots (QDs) with their unique optical and chemical properties, but also considerable ...
  • Investigating the interplay between sarcosine and Ca2+-dependent signaling in prostate cells 

    Strmiska, Vladislav; Buchtelová, Hana; Michálek, Petr; Křížková, Soňa; Adam, Vojtěch; Heger, Zbyněk (Mendel University in Brno, 2019-01-02)
    It has been shown that sarcosine supplementation stimulates proliferation and invassivenes of prostate cells. Nevertheless, the exact molecular mechanism responsible for this phenomenon is not known. In the present study ...
  • MALDI MSI of MeLiM melanoma: Searching for differences in protein profiles 

    Guráň, Roman; Pompeiano Vaníčková, Lucie; Horák, Vratislav; Křížková, Soňa; Michálek, Petr; Heger, Zbyněk; Zítka, Ondřej; Adam, Vojtěch (PLOS, 2017-12-08)
    Background Treatment of advanced cutaneous melanoma remains challenging, and new data on melanoma biology are required. The most widely accepted criteria for the prognostic evaluation of melanoma are histopathological and ...
  • Možnosti návrhu databáze SQL v MATLABU a realizace databázové struktury pro ukládáni výsledků měření 

    Michálek, Petr
    Tato práce se zabývá možností vyžití databázových systémů SQL s programem MATLAB. Úkolem bylo provést průzkum na trhu v oblasti SQL databází, porovnat různé systémy a jejich implementaci. Cílem praktické části je potom ...
  • Optimization of multiplex RT-PCR for selected isoforms of metallothionein genes and influence of cisplatin on prostatic cell lines 

    Petrlák, František; Šmídová, Veronika; Šplíchal, Zbyněk; Michálek, Petr (Mendel University in Brno, 2019-12-31)
    This study deals with the issue of metallothioneins as potential tumor markers using the multiplex reverse transcription polymerase chain reaction (RT-PCR) method, for which specific primers were designed, with the subsequent ...
  • Ruthenium-based core-shell nanoparticles with exceptional in vitro biocompatibility 

    Buchtelová, Hana; Strmiska, Vladislav; Dostálová, Simona; Michálek, Petr; Křížková, Soňa; Kopel, Pavel; Hynek, David; Richtera, Lukáš; Adam, Vojtěch; Heger, Zbyněk (Mendel University in Brno, 2017-12-31)
    The current study demonstrates design preparation and characterization of biocompatible hybrid ruthenium core-shell nanoparticles (RuNPs) coated with polyvinylpyrrolidone (PVP) and polyoxyethylene stearate (POES). The ...
  • Sarcosine degradation pathway is involved in the epigenetics of prostate cells 

    Strmiska, Vladislav; Michálek, Petr; Buchtelová, Hana; Lacková, Zuzana; Guráň, Roman; Křížková, Soňa; Pompeiano Vaníčková, Lucie; Zítka, Ondřej; Stiborová, Marie; Eckschlager, Tomáš; Adam, Vojtěch; Heger, Zbyněk (Mendel University in Brno, 2017-12-31)
    It has been shown that sarcosine suplementation stimulates the proliferation of prostate cells and also their invassiveness. In present study we show that enzymes conected with sarcosine conversion to glycine (sarcosine ...
  • Sarcosine is a prostate epigenetic modifier that elicits aberrant methylation patterns through the SAMe-Dnmts axis 

    Strmiska, Vladislav; Michálek, Petr; Lacková, Zuzana; Guráň, Roman; Křížková, Soňa; Pompeiano Vaníčková, Lucie; Zítka, Ondřej; Stiborová, Marie; Eckschlager, Tomáš; Klejdus, Bořivoj; Pacík, Dalibor; Tvrdíková, Eliška; Keil, Claudia; Haase, Hajo; Adam, Vojtěch; Heger, Zbyněk (FEBS Press, 2019-05-31)
    DNA hypermethylation is one of the most common epigenetic modifications in prostate cancer (PCa). Several studies have delineated sarcosine as a PCa oncometabolite that increases the migration of malignant prostate cells ...
  • Sarcosine up-regulates expression of genes involved in cell cycle progression of metastatic models of prostate cancer 

    Heger, Zbyněk; Merlos Rodrigo, Miguel Ángel; Michálek, Petr; Polanská, Hana; Masařík, Michal; Vít, Vítězslav; Plevová, Mariana; Pacík, Dalibor; Eckschlager, Tomáš; Stiborová, Marie; Adam, Vojtěch (PLOS, 2016-11-08)
    The effects of sarcosine on the processes driving prostate cancer (PCa) development remain still unclear. Herein, we show that a supplementation of metastatic PCa cells (androgen independent PC-3 and androgen dependent ...
  • Sledování vlivu použité autochtonní kultury kvasinek na kvasný proces výroby vína 

    Michálek, Petr
    Tato diplomová práce se zabývá identifikací vinných kvasinek izolovaných ze zakvašeného vinného moštu. Kvasinky byly izolovány z moštu odrůdy Rulandského modrého, které bylo pěstováno a vyráběno podle poţadavků kladených ...
  • Specific Magnetic Isolation of E6 HPV16 Modified Magnetizable Particles Coupled with PCR and Electrochemical Detection 

    Jimenez Jimenez, Ana Maria; Ruttkay-Nedecký, Branislav; Dostálová, Simona; Krejčová, Ludmila; Richtera, Lukáš; Adam, Vojtěch; Michálek, Petr (MDPI, 2016-05-01)
    The majority of carcinomas that were developed due to the infection with human papillomavirus (HPV) are caused by high-risk HPV types, HPV16 and HPV18. These HPV types contain the E6 and E7 oncogenes, so the fast detection ...
  • Staphylococcus aureus and MRSA Growth and Biofilm Formation after Treatment with Antibiotics and SeNPs 

    Číhalová, Kristýna; chudobova, Dagmar; Kizek, René; Michálek, Petr; Moulick, Amitava; Guráň, Roman; Kopel, Pavel; Adam, Vojtěch (MDPI, 2015-10-16)
    Methicillin-resistant Staphylococcus aureus (MRSA) is a dangerous pathogen resistant to -lactam antibiotics. Due to its resistance, it is difficult to manage the infections caused by this strain. We examined this issue in ...
  • Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients Swab 

    Komínková, Markéta; Michálek, Petr; Číhalová, Kristýna; Guráň, Roman; Cernei, Natalia Vladimirovna; Nejdl, Lukáš; Šmerková, Kristýna; Dostálová, Simona; Hegerová, Dagmar; Heger, Zbyněk; Veselý, Radek; Gumulec, Jaromír; Kynický, Jindřich; Xhaxhiu, Kledi; Zítka, Ondřej; Adam, Vojtěch; Kizek, René (MDPI, 2015-03-31)
    In this work, we focused on the differences between bacterial cultures of E. coli obtained from swabs of infectious wounds of patients compared to laboratory E. coli. In addition, blocking of the protein responsible for ...
  • Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients’ Swabs 

    Komínková, Markéta; Kizek, René; Michálek, Petr; Číhalová, Kristýna; Guráň, Roman; Cernei, Natalia Vladimirovna; Nejdl, Lukáš; Šmerková, Kristýna; Dostálová, Simona; chudobova, Dagmar; Heger, Zbyněk; Veselý, Radek; Gumulec, Jaromír; Kynický, Jindřich; Xhaxhiu, Kledi; Zítka, Ondřej; Adam, Vojtěch (MDPI, 2015-05-31)
    V této práci jsme se zaměřili na rozdíly mezi bakteriálních kultur E. coli, získaných ze stěrů infekčních ran pacientů ve srovnání s laboratorním E. coli. Navíc blokování proteinu odpovědné za syntézu glutathion ...