Browsing by Author "Zítka, Ondřej"
Now showing items 1-20 of 37
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Anticancerogenic effect of spices due to phenolic and flavonoid compounds – in vitro evaluation on prostate cells
Lacková, Zuzana; Buchtelová, Hana; Buchtová, Žaneta; Klejdus, Bořivoj; Heger, Zbyněk; Brtnický, Martin; Kynický, Jindřich; Zítka, Ondřej; Adam, Vojtěch (MDPI, 2017-09-28)This study shows the effects of spices, and their phenolic and flavonoid compounds, on prostate cell lines (PNT1A, 22RV1 and PC3). The results of an MTT assay on extracts from eight spices revealed the strongest inhibitory ... -
The Application of Curve Fitting on the Voltammograms of Various Isoforms of Metallothioneins–Metal Complexes
Merlos Rodrigo, Miguel Ángel; Jimenez Jimenez, Ana Maria; Zítka, Ondřej; Richtera, Lukáš; Adam, Vojtěch (MDPI, 2017-03-01)The translation of metallothioneins (MTs) is one of the defense strategies by which organisms protect themselves from metal-induced toxicity. MTs belong to a family of proteins comprising MT-1, MT-2, MT-3, and MT-4 classes, ... -
Are Early Somatic Embryos of the Norway Spruce (Picea abies (L.) Karst.) Organised?
Petřek, Jiří; Zítka, Ondřej; Adam, Vojtěch; Bartušek, Karel; Anjum, Naser A.; Pereira, Eduarda; Havel, Ladislav; Kizek, René (PLOS, 2015-12-01)Somatic embryogenesis in conifer species has great potential for the forestry industry. Hence, a number of methods have been developed for their efficient and rapid propagation through somatic embryogenesis. Although ... -
Behaviour of zinc complexes and zinc sulphide nanoparticles revealed by using screen printed electrodes and spectrometry
Nejdl, Lukáš; Ruttkay-Nedecký, Branislav; Kudr, Jiří; Kremplová, Monika; Cernei, Natalia Vladimirovna; Prášek, Jan; Konečná, Marie; Hubálek, Jaromír; Zítka, Ondřej; Kynický, Jindřich; Kopel, Pavel; Kizek, René; Adam, Vojtěch (MDPI AQ, 2013-10-25)In this study, we focused on microfluidic electrochemical analysis of zinc complexes (Zn(phen)(his)Cl-2, Zn(his)Cl-2) and ZnS quantum dots (QDs) using printed electrodes. This method was chosen due to the simple (easy to ... -
Bio-Sensing of Cadmium(II) Ions Using Staphylococcus aureus
Sochor, Jiří; Zítka, Ondřej; Hynek, David; Krejčová, Ludmila; Trnková, Libuše; Adam, Vojtěch; Hubálek, Jaromír; Kynický, Jindřich; Vrba, Radimír; Kizek, René (MDPI, 2011-11-08)Cadmium, as a hazardous pollutant commonly present in the living environment, represents an important risk to human health due to its undesirable effects (oxidative stress, changes in activities of many enzymes, interactions ... -
Comparison of biogenic amines and mycotoxins in alfalfa and red clover fodder depending on additives
Adam, Vojtěch; Zítka, Ondřej (MDPI, 2017-04-30)In the production of fermented feed, each crop can be contaminated with a variety of microorganisms that may produce natural pollutants. Biogenic amines, mycotoxins, and undesirable organic acids can decrease health feed ... -
Content of phenolic compounds and antioxidant capacity in fruits of selected genotypes of apricot with resistance against Plum pox virus
Sochor, Jiří; Zítka, Ondřej; Škutková, Helena; Pavlík, Dušan; Babula, Petr; Krška, Boris; Horna, Aleš; Adam, Vojtěch; Provazník, Ivo; Kizek, René (MDPI, 2010-09-07)Research on natural compounds is increasingly focused on their effects on human health. In this study, we were interested in the evaluation of nutritional value expressed as content of total phenolic compounds and antioxidant ... -
Determination of Histamine in Silages Using Nanomaghemite Core (-Fe2O3)-Titanium Dioxide Shell Nanoparticles Off-Line Coupled with Ion Exchange Chromatography
Cernei, Natalia Vladimirovna; Lacková, Zuzana; Guráň, Roman; Hynek, David; Skládanka, Jiří; Horký, Pavel; Zítka, Ondřej; Adam, Vojtěch (MDPI, 2016-09-12)The presence of biogenic amines is a hallmark of degraded food and its products. Herein, we focused on the utilization of magnetic nanoparticles off-line coupled with ion exchange chromatography with post-column ninhydrin ... -
Determination of hydroxyproline using ion-exchange liquid chromatography with vis detector and high performance liquid chromatography with fluorescence detector
Lacková, Zuzana; Cernei, Natalia Vladimirovna; Štěrbová, Dagmar; Haddad, Yazan Abdulmajeed Eyadh; Rozíková, Veronika; Komprda, Tomáš; Zítka, Ondřej (Mendel University in Brno, 2017-12-31)The first aim of the experiment was an optimization of the method for determination of hydroxyproline in a pig skin using high-performance liquid chromatography with fluorescence detector (HPLC-FLD) and ion-exchange liquid ... -
Determination of the content of capsaicin and dihydrocapsaicin in twelve varieties of chilli peppers using liquid chromatography with uv/vis detection
Do, Tomáš; Lacková, Zuzana; Adam, Vojtěch; Zítka, Ondřej (Mendel University in Brno, 2017-12-31)The aim of the experiment was to determine the content of capsaicin and dihydrocapsaicin in twelve varieties of chilli peppers (Brown Bhutlah II, Bhut Jolokia Yellow I, Pieto de Moca II, Trinidad 7 POT II, 7 POT White II, ... -
Differences in Urinary Amino Acid Patterns in Individuals with Different Types of Urological Tumor Urinary Amino Acid Patterns as Markers of Urological Tumors
Dušková, Kateřina; Veselý, Štěpán; Do Carmo Silva, Joana Isabel; Cernei, Natalia Vladimirovna; Zítka, Ondřej; Heger, Zbyněk; Adam, Vojtěch; Havlová, Klára; Babjuk, Marek (The International Institute of Anticancer Research, 2018-04-01)Background: Insufficient specificity and invasiveness of currently used diagnostic methods raises the need for new markers of urological tumors. The aim of this study was to find a link between the urinary excretion of ... -
The effect of coffee supplementation on glutathione and total thiols levels
Buchtová, Žaneta; Lacková, Zuzana; Kudr, Jiří; Adam, Vojtěch; Zítka, Ondřej (Mendel University in Brno, 2017-12-31)The effect of coffee supplementation on glutathione and total thiols levels Antioxidants are very important substances that counteract the formation of free radicals. They are divided into exogenous, such as vitamin C which ... -
Effect of five different stages of ripening on chemical compounds in medlar (Mespilus germanica L.)
Rop, Otakar; Sochor, Jiří; Juříková, Tunde; Zítka, Ondřej; Škutková, Helena; Mlček, Jiří; Salaš, Petr; Krška, Boris; Babula, Petr; Adam, Vojtěch; Kramářová, Daniela; Beklová, Miroslava; Provazník, Ivo; Kizek, René (MDPI, 2010-12-28)Study of changes of nutritional value of fruit during the process of ripening can help to estimate the optimal date for fruit harvesting to achieve the best quality for direct consumption and further utilization. The aim ... -
Effect of Magnetic Nanoparticles on Tobacco BY-2 Cell Suspension Culture
Kryštofová, Olga; Sochor, Jiří; Zítka, Ondřej; Babula, Petr; Kudrle, Vít; Adam, Vojtěch; Kizek, René (MDPI, 2013-01-01)Nanomaterials are structures whose exceptionality is based on their large surface, which is closely connected with reactivity and modification possibilities. Due to these properties nanomaterials are used in textile industry ... -
Effect of the selected phenolic and flavonoid compounds of black pepper and caraway seeds on prostate cells
Lacková, Zuzana; Buchtelová, Hana; Buchtová, Žaneta; Adam, Vojtěch; Zítka, Ondřej (Mendel University in Brno, 2017-12-31)In this study, the effect of selected phenolic and flavonoid compounds of black pepper and caraway seeds on prostate cells (PNT1A, 22RV1 and PC3) was observed. Synthetic standards of 3,4-dihydroxybenzaldehyde and naringenin ... -
Flow Injection Analysis with Electrochemical Detection for Rapid Identification of Platinum-Based Cytostatics and Platinum Chlorides in Water
Komínková, Markéta; Heger, Zbyněk; Zítka, Ondřej; Kynický, Jindřich; Pohanka, Miroslav; Beklová, Miroslava; Adam, Vojtěch; Kizek, René (MDPI, 2014-02-04)Platinum-based cytostatics, such as cisplatin, carboplatin or oxaliplatin are widely used agents in the treatment of various types of tumors. Large amounts of these drugs are excreted through the urine of patients into ... -
Influence of Long-Distance Bicycle Riding on Serum/Urinary Biomarkers of Prostate Cancer
Heger, Zbyněk; Gumulec, Jaromír; Ondrák, Aleš; Škoda, Jan; Zítka, Zdeněk; Cernei, Natalia Vladimirovna; Masařík, Michal; Zítka, Ondřej; Adam, Vojtěch (MDPI, 2016-03-17)Herein, we present a study focused on the determination of the influence of long-distance (53 km) bicycle riding on levels of chosen biochemical urinary and serum prostate cancer (PCa) biomarkers total prostate-specific ... -
Investigation into the Effect of Molds in Grasses on Their Content of Low Molecular Mass Thiols
Skládanka, Jiří; Adam, Vojtěch; Zítka, Ondřej; Kryštofová, Olga; Beklová, Miroslava; Kizek, René; Havlíček, Zdeněk; Sláma, Petr; Nawrath, adam (MDPI, 2012-10-24)The aim of this study was to investigate the effect of molds on levels of low molecular mass thiols in grasses. For this purpose, the three grass species Lolium perenne, Festulolium pabulare and Festulolium braunii were ... -
An Investigation of Glutathione-Platinum(II) Interactions by Means of the Flow Injection Analysis Using Glassy Carbon Electrode
Zítka, Ondřej; Křížková, Soňa; Adam, Vojtěch; Trnková, Libuše; Hubálek, Jaromír; Kizek, René (MDPI, 2007-07-20)Glutathione was discovered by M. J. de Rey Pailhade at the end of the 19th century as the substance (hydrogénant le soufre), which was renamed by F.G. Hopkins in 1921. Hopkins first characterised the compound as a dipeptide ... -
Ion exchange chromatography and mass spectrometric methods for analysis of cadmium-phytochelatin (II) complexes
Merlos Rodrigo, Miguel Ángel; Cernei, Natalia Vladimirovna; Komínková, Markéta; Zítka, Ondřej; Beklová, Miroslava; Zehnálek, Josef; Kizek, René; Adam, Vojtěch (MDPI, 2013-03-28)In this study, in vitro formed Cd-phytochelatin (PC2) complexes were characterized using ion exchange chromatography (IEC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The ...