MALDI-TOF MSI method for determining spatial distribution of infection markers in pulmonary tissues of pigs
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Date
2019-01-02
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Advisor
Referee
Mark
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Mendel University in Brno
Abstract
In recent years the matrix-assisted laser desorption/ionization time-of-flight mass
spectrometry imaging (MALDI-TOF MSI) is used for molecular mapping of diverse biomarkers such
as proteins or peptides in animal/plant tissue sections. It takes full advantage of the benefits of MALDITOF
technique which is the ability of rapid measurements of all mass spectra in a wide mass range and
detection of analytes molecular weights. Interleukins, the group of mostly proinflammatory cytokines,
are the proteins that are produced as immune response on bacterial infection Actinobacillus
pleuropneumonia. The aim of this study was to develop a MALDI-TOF MSI method for quantitative
visualization of spatial distribution of interleukins and other cell markers of lymphocytes, granulocytes
and macrophages in porcine tissues of lymph nodes and lungs. The determination of the spatial
distribution of produced proteins will bring further useful knowledge of the pathogenesis of this
economically important disease of pigs, which can also contribute to reducing the consumption of
antimicrobials.
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Peer-reviewed
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en
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(C) Mendel University in Brno