Investigating the interplay between sarcosine and Ca2+-dependent signaling in prostate cells
Abstract
It has been shown that sarcosine supplementation stimulates proliferation and invassivenes
of prostate cells. Nevertheless, the exact molecular mechanism responsible for this phenomenon is not
known. In the present study we demonstrate that sarcosine increases expression of calmodulin (CaM),
an important intracellular signaling molecule. Through this, sarcosine activates calmodulin-dependent
protein kinases signaling. Pathway of activation CaM-dependent protein kinases can activate
regulation of mitosis, proliferation, cell death, gene transcription and phosphorylation/
dephosphorlation of proteins. This is done through CaM binding of four Ca2+ ions. Interestingly, in
this study, we identified decrese in free Ca2+ correlating with sarcosine-induced up-regulation of CaM.
The influence of CaM to cell cycle changes was further verified using post transcritrional gene
silencing using CaM-siRNA complex. Co-treatment of prostate cells with CaM-siRNA and sarcosine
showed decrease in CaM-dependent kinases and cell invasiveness compared to sarcosine treatment
only.
Keywords
sarcosine, calmodulin, prostate, prostate cancer, human cellsPersistent identifier
http://hdl.handle.net/11012/178388Document type
Peer reviewedDocument version
Final PDFSource
MendelNet 2018. 2019, p. 532-536.https://mendelnet.cz/artkey/mnt-201801-0113_Investigating-the-interplay-between-sarcosine-and-Ca-sup-2-sup-dependent-signaling-in-prostate-cells.php?back=/magno/mnt/2018/mn1.php?secid=4
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