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Investigating the interplay between sarcosine and Ca2+-dependent signaling in prostate cells

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Investigating the interplay between sarcosine and Ca2dependent signaling in prostate cells.pdf (1.587Mb)
Date
2019-01-02
Author
Strmiska, Vladislav
Buchtelová, Hana
Michálek, Petr
Křížková, Soňa
Adam, Vojtěch
Heger, Zbyněk
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Abstract
It has been shown that sarcosine supplementation stimulates proliferation and invassivenes of prostate cells. Nevertheless, the exact molecular mechanism responsible for this phenomenon is not known. In the present study we demonstrate that sarcosine increases expression of calmodulin (CaM), an important intracellular signaling molecule. Through this, sarcosine activates calmodulin-dependent protein kinases signaling. Pathway of activation CaM-dependent protein kinases can activate regulation of mitosis, proliferation, cell death, gene transcription and phosphorylation/ dephosphorlation of proteins. This is done through CaM binding of four Ca2+ ions. Interestingly, in this study, we identified decrese in free Ca2+ correlating with sarcosine-induced up-regulation of CaM. The influence of CaM to cell cycle changes was further verified using post transcritrional gene silencing using CaM-siRNA complex. Co-treatment of prostate cells with CaM-siRNA and sarcosine showed decrease in CaM-dependent kinases and cell invasiveness compared to sarcosine treatment only.
Keywords
sarcosine, calmodulin, prostate, prostate cancer, human cells
Persistent identifier
http://hdl.handle.net/11012/178388
Document type
Peer reviewed
Document version
Final PDF
Source
MendelNet 2018. 2019, p. 532-536.
https://mendelnet.cz/artkey/mnt-201801-0113_Investigating-the-interplay-between-sarcosine-and-Ca-sup-2-sup-dependent-signaling-in-prostate-cells.php?back=/magno/mnt/2018/mn1.php?secid=4
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