Isolation of Arginine Using Maghemite Particles
Abstract
In this paper, we report the optimal conditions for the isolation of amino acid arginine from different types
of matrix, such as blood, plasma or tissue and their characterization using ion-exchange liquid chromatography
with VIS detector. Paramagnetic microparticles able to isolate and immobilize amino acid arginine were
prepared and used to preconcentrate arginine for subsequent analysis on ion-exchange liquid chromatography
with VIS detector. Commercially available Dowex was covered by maghemite nanoparticles and the composite
showed excellent binding and superparamagnetic properties. It was found that the paramagnetic
microparticles were able to bind specifically amino acid arginine. Therefore, these paramagnetic microparticles
have potential for better isolation of arginine and in future can use for application as a platform of delivery
system.
Persistent identifier
http://hdl.handle.net/11012/178393Document type
Peer reviewedDocument version
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- Chytré nanonástroje [213]