Isolation of Arginine Using Maghemite Particles
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In this paper, we report the optimal conditions for the isolation of amino acid arginine from different types of matrix, such as blood, plasma or tissue and their characterization using ion-exchange liquid chromatography with VIS detector. Paramagnetic microparticles able to isolate and immobilize amino acid arginine were prepared and used to preconcentrate arginine for subsequent analysis on ion-exchange liquid chromatography with VIS detector. Commercially available Dowex was covered by maghemite nanoparticles and the composite showed excellent binding and superparamagnetic properties. It was found that the paramagnetic microparticles were able to bind specifically amino acid arginine. Therefore, these paramagnetic microparticles have potential for better isolation of arginine and in future can use for application as a platform of delivery system.
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Zdrojový dokumentNANOCON 2018. 2019, p. 210-213.
- Chytré nanonástroje