Use of the industrial X-ray computed microtomography to address scientific questions in developmental biology
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X-ray computed microtomography (microCT) that allows to extract quantitative 3D information about various objects is already a well-established and widely used industrial technique for non-destructive testing, materials characterization and dimensional metrology. On the other hand, modern developmental biology requires both qualitative and quantitative three-dimensional information about the studied objects, which is not provided by conventional 2D imaging methods. In addition, the complexity of biological structures often requires a more comprehensive methodology to compare shapes and sizes. Our approach is to connect these two worlds, i.e. to apply procedures and analysis routinely used or developed for industrial computed tomography to address scientific questions in developmental biology. This approach, which is based on the long-term experience of the CEITEC BUT Brno micro/nanoCT research team on both, industrial and scientific application of X-ray microCT, brings new possibilities in imaging, data processing and evaluation of 3D biological models. Here we present 3D structures that were analysed by different approaches on the selected examples from developmental biology. In addition, quantitative analysis usually used in industry were applied to segmented 3D models e.g. wall thickness analysis of facial cartilage, shape comparisons of nasal capsules, automatic quantification of the cells in the salamander limb etc. A complete procedure consisting of staining, microCT measurement and subsequent data processing is discussed.
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Zdrojový dokumentThe e-Journal of Nondestructive Testing. 2019, vol. 24, issue 3, p. 1-9.