LA-ICP-MS as a sensitive method for detection of nanoparticle-antibody conjugates in immunochemistry analysis

Laser ablation followed by inductively coupled plasma mass spectrometry is an analytical method suitable for low concentration measurement of elements in a sample. This can be used not only for detection of elemental distribution within a sample but also as a detection means for immunoanalytical techniques using nanoparticle-antibody conjugates as recognition elements. The aim of this work was to prepare a conjugate of 10nm gold nanoparticles with anti-p53 antibody, to verify the antigen binding ability, and to use this construct for identification of p53 in a real sample of cell lysate. It has been experimentally verified that it is possible to use nanoparticle-modified antibodies for immunochemical analysis coupled to LA-ICP-MS detection. However, the nonspecific sorption of nanoparticles on the sample surface needs to be minimized by optimization of the blocking step.

Keywords

gold nanoparticles, LA-ICP-MS, immunochemistry, dot-blot, protein detection, p53

Citation

MendelNet 2019 Proceedings of 26th International PhD Students Conference. 2019, p. 682-686.
https://mendelnet.cz/artkey/mnt-201901-0131_LA-ICP-MS-as-a-sensitive-method-for-detection-of-nanoparticle-antibody-conjugates-in-immunochemistry-analysis.php?back=/magno/mnt/2019/mn1.php?secid=4

Document type

Peer-reviewed

Document version

Published version

Language of document

en

Document licence

(C) Mendel University in Brno