Analysis of high frame-rate movies by variational methods
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The object of this study was the recognition of Regions Of Interest(ROIs)in a time series of digital images of two specific laboratory experiments. It concernsthe identification of objects in a tissue surface by high-resolution and high-speedad-hoc systems for morphological dynamic image analysis. The protocols and thealgorithms implemented are developed to retrieve biomechanical properties of twodifferentin vitrosystems; the solid filament X-MET (eX-vivo Muscle EngineeredTissue) to measure its reaction to a different frequency stimulation, and a planarsystem of co-culture of skeletal and cardiac muscle cells, where myotubes and car-diomyocytes coexist, to discriminate the interaction between different cell’s type,of its spontaneous pulse. The results of the stimulated X-MET from solid culturesystem are frequency dependent points of the macroscopic muscular strength andits contractile response. The results for the co-culture planar board measure thecorrelation of the pulsed movements of the different parts of the tissue.
Document typePeer reviewed
Document versionFinal PDF
SourceMathematics for Applications. 2019 vol. 8, č. 1, s. 59-77. ISSN 1805-3629
- 2019/1