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dc.contributor.authorVasylkivska, Marynacs
dc.contributor.authorJurečková, Kateřinacs
dc.contributor.authorBranská, Barboracs
dc.contributor.authorSedlář, Karelcs
dc.contributor.authorKolek, Jancs
dc.contributor.authorProvazník, Ivocs
dc.contributor.authorPatáková, Petracs
dc.date.accessioned2020-08-04T11:00:13Z
dc.date.available2020-08-04T11:00:13Z
dc.date.issued2019-11-07cs
dc.identifier.citationPLOS ONE. 2019, vol. 14, issue 11, p. 1-19.en
dc.identifier.issn1932-6203cs
dc.identifier.other159469cs
dc.identifier.urihttp://hdl.handle.net/11012/193230
dc.description.abstractIn-depth knowledge of cell metabolism and nutrient uptake mechanisms can lead to the development of a tool for improving acetone-butanol-ethanol (ABE) fermentation performance and help to overcome bottlenecks in the process, such as the high cost of substrates and low production rates. Over 300 genes potentially encoding transport of amino acids, metal ions, vitamins and carbohydrates were identified in the genome of the butanol-producing strain Clostridium beijerinckii NRRL B-598, based on similarity searches in protein function databases. Transcriptomic data of the genes were obtained during ABE fermentation by RNA-Seq experiments and covered acidogenesis, solventogenesis and sporulation. The physiological roles of the selected 81 actively expressed transport genes were established on the basis of their expression profiles at particular stages of ABE fermentation. This article describes how genes encoding the uptake of glucose, iron, riboflavin, glutamine, methionine and other nutrients take part in growth, production and stress responses of C. beijerinckii NRRL B-598. These data increase our knowledge of transport mechanisms in solventogenic Clostridium and may be used in the selection of individual genes for further research.en
dc.formattextcs
dc.format.extent1-19cs
dc.format.mimetypeapplication/pdfcs
dc.language.isoencs
dc.publisherPLOScs
dc.relation.ispartofPLOS ONEcs
dc.relation.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0224560cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectClostridium beijerinckii NRRL B-598en
dc.subjectRNA-Seqen
dc.titleTranscriptional analysis of amino acid, metal ion, vitamin and carbohydrate uptake in butanol-producing Clostridium beijerinckii NRRL B-598en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrstvícs
sync.item.dbidVAV-159469en
sync.item.dbtypeVAVen
sync.item.insts2021.02.25 16:53:11en
sync.item.modts2021.02.25 16:13:38en
dc.coverage.issue11cs
dc.coverage.volume14cs
dc.identifier.doi10.1371/journal.pone.0224560cs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1932-6203/cs
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen


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Creative Commons Attribution 4.0 International
Except where otherwise noted, this item's license is described as Creative Commons Attribution 4.0 International