Automated alignment method for coherence-controlled holographic microscope
Automatizovaná metoda ladění pro koherenci řízenou holografickou mikroskopii
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Coherence-controlled holographic microscope (CCHM) was developed particularly for quantitative phase imaging and measurement of live cell dynamics, which is the proper subject of digital holographic microscopy (DHM). CCHM in low-coherence mode extends DHM in the study of living cells. However, this advantage is compensated by sensitivity of the system to easily become misaligned, which is a serious hindrance to wanted performance. Therefore, it became clear that introduction of a self-correcting system is inevitable. Accordingly, we had to devise a theory of a suitable control and design an automated alignment system for CCHM. The modulus of the reconstructed holographic signal was identified as a significant variable for guiding the alignment procedures. From this, we derived the original basic realignment three-dimensional algorithm, which encompasses a unique set of procedures for automated alignment that contains processes for initial and advanced alignment as well as long-term maintenance of microscope tuning. All of these procedures were applied to a functioning microscope and the tested processes were successfully validated. Finally, in such a way, CCHM is enabled to substantially contribute to study of biology, particularly of cancer cells in vitro.V článku je popsaná automatická metoda ladění pro koherencí řízenou holografickou mikroskopii. Všechny popsané procedury byly aplikovány na fungující mikroskop a testované procesy byly úspěšně ověřeny.
Keywordsholographic microscopy, quantitative phase imaging, automated alignment, holographic signal, holografická mikroskopie, kvantitativní fázové zobrazování, automatizované ladění, holografický signál
Document typePeer reviewed
Document versionFinal PDF
SourceJOURNAL OF BIOMEDICAL OPTICS. 2015, vol. 20, issue 11, p. 1-8.