Hydroxyproline assay by HPLC-FLD applied for wound healing determination in rat model
Loading...
Date
2019-12-31
ORCID
Advisor
Referee
Mark
Journal Title
Journal ISSN
Volume Title
Publisher
Mendel University in Brno
Abstract
In this work, we focused on four types of oils and their response on wound healing of the skin of rats after dietary exposure. A set of samples consisted of 48 adult male rats of Wistar albino, which were divided into four groups according to the respective oil. The content of hydroxyproline in the skin tissue, used as a biomarker for wound healing, was determined by high-performance liquid chromatography with a fluorescence detector. There was found out, that the highest content of hydroxyproline was detected in the group of rats, which have been fed with the addition of palm oil (118.4 ± 1.9 µg/g). These results indicate that palm oil had the most positive effect on wound healing.
Description
Citation
MendelNet 2019 Proceedings of 26th International PhD Students Conference. 2019, p. 579-584.
https://mendelnet.cz/artkey/mnt-201901-0111_Hydroxyproline-assay-by-HLPC-FLD-applied-for-wound-healing-determination-in-rat-model.php?back=/magno/mnt/2019/mn1.php?secid=4
https://mendelnet.cz/artkey/mnt-201901-0111_Hydroxyproline-assay-by-HLPC-FLD-applied-for-wound-healing-determination-in-rat-model.php?back=/magno/mnt/2019/mn1.php?secid=4
Document type
Peer-reviewed
Document version
Published version
Date of access to the full text
Language of document
en
Study field
Comittee
Date of acceptance
Defence
Result of defence
Document licence
(C) Mendel University in Brno