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dc.contributor.authorMazumdar, Anindacs
dc.contributor.authorHaddad, Yazan Abdulmajeed Eyadhcs
dc.contributor.authorSur, Vishma Pratapcs
dc.contributor.authorMilosavljević, Vedrancs
dc.contributor.authorBhowmick, Sukanyacs
dc.contributor.authorMichálková, Hanacs
dc.contributor.authorGuráň, Romancs
dc.contributor.authorVeselý, Radekcs
dc.contributor.authorMoulick, Amitavacs
dc.date.accessioned2021-02-08T11:57:36Z
dc.date.available2021-02-08T11:57:36Z
dc.date.issued2020-08-25cs
dc.identifier.citationFrontiers in Microbiology. 2020, vol. 11, issue 1, p. 1-19.en
dc.identifier.issn1664-302Xcs
dc.identifier.other169138cs
dc.identifier.urihttp://hdl.handle.net/11012/196325
dc.description.abstractAn inexorable switch from antibiotics has become a major desideratum to overcome antibiotic resistance. Bacteriocin fromLactobacillus casei, a cardinal probiotic was used to design novel antibacterial peptides named as Probiotic Bacteriocin Derived and Modified (PBDM) peptides (PBDM1: YKWFAHLIKGLC and PBDM2: YKWFRHLIKKLC). The loop-shaped 3D structure of peptides was characterizedin silicovia molecular dynamics simulation as well as biophysically via spectroscopic methods. Thereafter,in vitroresults against multidrug resistant bacterial strains and hospital samples demonstrated the strong antimicrobial activity of PBDM peptides. Further,in vivostudies with PBDM peptides showed downright recovery of balb/c mice from Vancomycin ResistantStaphylococcus aureus(VRSA) infection to its healthy condition. Thereafter,in vitrostudy with human epithelial cells showed no significant cytotoxic effects with high biocompatibility and good hemocompatibility. In conclusion, PBDM peptides displayed significant antibacterial activity against certain drug resistant bacteria which cause infections in human beings. Future analysis are required to unveil its mechanism of action in order to execute it as an alternative to antibiotics.en
dc.formattextcs
dc.format.extent1-19cs
dc.format.mimetypeapplication/pdfcs
dc.language.isoencs
dc.publisherFrontiers Mediacs
dc.relation.ispartofFrontiers in Microbiologycs
dc.relation.urihttps://doi.org/10.3389/fmicb.2020.01963cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectantibacterial peptidesen
dc.subjectantibioticsen
dc.subjectmultidrug resistanceen
dc.subjectbacteriaen
dc.subjectinfectionsen
dc.titleCharacterization and in vitro analysis of probiotic-derived peptides against multi drug resistance bacterial infectionsen
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
sync.item.dbidVAV-169138en
sync.item.dbtypeVAVen
sync.item.insts2021.02.08 12:57:36en
sync.item.modts2021.02.08 12:16:09en
dc.coverage.issue1cs
dc.coverage.volume11cs
dc.identifier.doi10.3389/fmicb.2020.01963cs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1664-302X/cs
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen


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Creative Commons Attribution 4.0 International
Except where otherwise noted, this item's license is described as Creative Commons Attribution 4.0 International