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dc.contributor.authorVojtová, Lucycs
dc.contributor.authorPavliňáková, Veronikacs
dc.contributor.authorMuchová, Johanacs
dc.contributor.authorKacvinská, Katarínacs
dc.contributor.authorBrtníková, Janacs
dc.contributor.authorKnoz, Martincs
dc.contributor.authorLipový, Břetislavcs
dc.contributor.authorFaldyna, Martincs
dc.contributor.authorGöpfert, Eduardcs
dc.contributor.authorHoloubek, Jakubcs
dc.contributor.authorPavlovský, Zdeněkcs
dc.contributor.authorVícenová, Monikacs
dc.contributor.authorBlahnová, Veronikacs
dc.contributor.authorHearnden, Vanessacs
dc.contributor.authorFilova, Evacs
dc.date.accessioned2021-07-14T06:53:26Z
dc.date.available2021-07-14T06:53:26Z
dc.date.issued2021-06-01cs
dc.identifier.citationBiomedicines. 2021, vol. 9, issue 6, p. 1-27.en
dc.identifier.issn2227-9059cs
dc.identifier.other171955cs
dc.identifier.urihttp://hdl.handle.net/11012/200493
dc.description.abstractWound healing is a process regulated by a complex interaction of multiple growth factors including fibroblast growth factor 2 (FGF2). Although FGF2 appears in several tissue engineered studies, its applications are limited due to its low stability both in vitro and in vivo. Here, this shortcoming is overcome by a unique nine-point mutant of the low molecular weight isoform FGF2 retaining full biological activity even after twenty days at 37 degrees C. Crosslinked freeze-dried 3D porous collagen/chitosan scaffolds enriched with this hyper stable recombinant human protein named FGF2-STAB(R) were tested for in vitro biocompatibility and cytotoxicity using murine 3T3-A31 fibroblasts, for angiogenic potential using an ex ovo chick chorioallantoic membrane assay and for wound healing in vivo with 3-month old white New Zealand rabbits. Metabolic activity assays indicated the positive effect of FGF2-STAB(R) already at very low concentrations (0.01 mu g/mL). The angiogenic properties examined ex ovo showed enhanced vascularization of the tested scaffolds. Histological evaluation and gene expression analysis by RT-qPCR proved newly formed granulation tissue at the place of a previous skin defect without significant inflammation infiltration in vivo. This work highlights the safety and biocompatibility of newly developed crosslinked collagen/chitosan scaffolds involving FGF2-STAB(R) protein. Moreover, these sponges could be used as scaffolds for growing cells for dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.en
dc.formattextcs
dc.format.extent1-27cs
dc.format.mimetypeapplication/pdfcs
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofBiomedicinescs
dc.relation.urihttps://www.mdpi.com/2227-9059/9/6/590cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectcollagenen
dc.subjectchitosanen
dc.subjectscaffolden
dc.subjectFGF2en
dc.subjectskin regenerationen
dc.subjecttissue engineeringen
dc.titleHealing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB(R) Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluationen
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé biomateriálycs
sync.item.dbidVAV-171955en
sync.item.dbtypeVAVen
sync.item.insts2021.11.02 00:52:24en
sync.item.modts2021.11.02 00:14:10en
dc.coverage.issue6cs
dc.coverage.volume9cs
dc.identifier.doi10.3390/biomedicines9060590cs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2227-9059/cs
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen


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Creative Commons Attribution 4.0 International
Except where otherwise noted, this item's license is described as Creative Commons Attribution 4.0 International