Imaging of native early embryogenic tissue of Scots pine (Pinus sylvestris L.) by ESEM.
Environmental scanning electron microscopy enables the investigation of uncoated pine early embryogenic tissue samples in situ. The samples were examined under low vacuum conditions (air pressure 550 Pa) at a temperature of around -18°C by the AQUASEM II noncommercial environmental scanning electron microscope. The native extracellular matrix surface network was imaged by the environmental scanning electron microscope and in dark field mode of the optical microscope too. The backscattered electron detector disclosed brightness loci in the cells of early embryogenic culture. This work shows images of native pine embryogenic tissues. The continuity of extracellular matrix with structural integrity of plant organism is discussed.
Keywordssomatic embryogenesis * backscattered electron detector * ionization detector * bottle cells * extracellular matrix * structural integrity
Document typePeer reviewed
Document versionFinal PDF
SourceOpen Life Sciences. 2015, vol. 10, issue 1, p. 285-290.