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Efficient Protein Transfection by Swarms of Chemically Powered Plasmonic Virus-Sized Nanorobots

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Date
2021-08-24
Author
Ressnerová, Alžběta
Novotný, Filip
Michálková, Hana
Pumera, Martin
Adam, Vojtěch
Heger, Zbyněk
Altmetrics
10.1021/acsnano.1c01172
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Abstract
Transfection is based on nonviral delivery of nucleic acids or proteins into cells. Viral approaches are being used; nevertheless, their translational capacity is nowadays decreasing due to persistent fear of their safety, therefore creating space for the field of nanotechnology. However, nanomedical approaches introducing static nanoparticles for the delivery of biologically active molecules are very likely to be overshadowed by the vast potential of nanorobotics. We hereby present a rapid nonviral transfection of protein into a difficult-to-transfect prostate cancer cell line facilitated by chemically powered rectangular virus-sized (68 nm x 33 nm) nanorobots. The enhanced diffusion of these biocompatible nanorobots is the key to their fast internalization into cells, happening in a matter of minutes and being up to 6-fold more efficient compared to static nanorobots in a nonfueled environment. The Au/Ag plasmonic nature of these nanorobots makes them simply traceable and allows for their detailed subcellular localization. Protein transfection mediated by such nanorobots is an important step forward, challenging the field of nanomedicine and having potential in future translational medical research.
Keywords
transfection, nanorobots, metallothionein, protein delivery, protein transfection
Persistent identifier
http://hdl.handle.net/11012/203120
Document type
Peer reviewed
Document version
Postprint
Fulltext will be available on 21. 07. 2022
Source
ACS Nano. 2021, vol. 15, issue 8, p. 12899-12910.
https://pubs.acs.org/doi/10.1021/acsnano.1c01172
DOI
10.1021/acsnano.1c01172
Collections
  • Chytré nanonástroje [306]
  • Energie budoucnosti a inovace [38]
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