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dc.contributor.authorPekárková, Janacs
dc.contributor.authorGablech, Imrichcs
dc.contributor.authorFialová, Tatianacs
dc.contributor.authorBílek, Ondřejcs
dc.contributor.authorFohlerová, Zdenkacs
dc.date.accessioned2022-01-14T15:56:27Z
dc.date.available2022-01-14T15:56:27Z
dc.date.issued2021-12-03cs
dc.identifier.citationFrontiers in Bioengineering and Biotechnology. 2021, vol. 9, issue 1, p. 1-11.en
dc.identifier.issn2296-4185cs
dc.identifier.other175415cs
dc.identifier.urihttp://hdl.handle.net/11012/203324
dc.description.abstractParylene-based implants or coatings introduce surfaces suffering from bacteria colonization. Here, we synthesized polyvinylpyrrolidone-stabilized selenium nanoparticles (SeNPs) as the antibacterial agent, and various approaches are studied for their reproducible adsorption, and thus the modification of parylene-C-coated glass substrate. The nanoparticle deposition process is optimized in the nanoparticle concentration to obtain evenly distributed NPs on the flat parylene-C surface. Moreover, the array of parylene-C micropillars is fabricated by the plasma etching of parylene-C on a silicon wafer, and the surface is modified with SeNPs. All designed surfaces are tested against two bacterial pathogens, Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive). The results show no antibacterial effect toward S. aureus, while some bacteriostatic effect is observed for E. coli on the flat and microstructured parylene. However, SeNPs did not enhance the antibacterial effect against both bacteria. Additionally, all designed surfaces show cytotoxic effects toward mesenchymal stem cells at high SeNP deposition. These results provide valuable information about the potential antibacterial treatment of widely used parylene-C in biomedicine.en
dc.formattextcs
dc.format.extent1-11cs
dc.format.mimetypeapplication/pdfcs
dc.language.isoencs
dc.publisherFRONTIERS MEDIA SAcs
dc.relation.ispartofFrontiers in Bioengineering and Biotechnologycs
dc.relation.urihttps://www.frontiersin.org/articles/10.3389/fbioe.2021.782799/fullcs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectparylene-Cen
dc.subjectmicropillarsen
dc.subjectselenium nanoparticlesen
dc.subjectbiocompatibilityen
dc.subjectantimicrobialen
dc.titleModifications of Parylene by Microstructures and Selenium Nanoparticles: Evaluation of Bacterial and Mesenchymal Stem Cell Viabilityen
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav mikroelektronikycs
sync.item.dbidVAV-175415en
sync.item.dbtypeVAVen
sync.item.insts2022.01.14 16:56:27en
sync.item.modts2022.01.14 16:15:18en
dc.coverage.issue1cs
dc.coverage.volume9cs
dc.identifier.doi10.3389/fbioe.2021.782799cs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2296-4185/cs
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen


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Creative Commons Attribution 4.0 International
Except where otherwise noted, this item's license is described as Creative Commons Attribution 4.0 International