Browsing Fakulta elektrotechniky a komunikačních technologií by Author "Patáková, Petra"
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Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in Clostridium beijerinckii NRRL B-598 at the transcriptomic level
Patáková, Petra; Branská, Barbora; Sedlář, Karel; Vasylkivska, Maryna; Jurečková, Kateřina; Kolek, Jan; Cicková, Pavlína; Provazník, Ivo (Springer Nature, 2019-02-04)Clostridium beijerinckii NRRL B-598 is a sporulating, butanol and hydrogen producing strain that utilizes carbohydrates by the acetone-butanol-ethanol (ABE) fermentative pathway. The pathway consists of two metabolic phases, ... -
Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporation
Kolek, Jan; Sedlář, Karel; Provazník, Ivo; Patáková, Petra (BioMed Central, 2016-01-20)Butanol is currently one of the most discussed biofuels. Its use provides many benefits in comparison to bio-ethanol, but the price of its fermentative production is still high. Genetic improvements could help solve many ... -
Deeper below the surface – transcriptional changes of selected Clostridium beijerinckii NRRL B-598 genes induced by butanol shock
Patáková, Petra; Kolek, Jan; Jurečková, Kateřina; Branská, Barbora; Sedlář, Karel; Vasylkivska, Maryna; Provazník, Ivo (John Wiley & Sons, 2020-12-14)The main bottleneck in the return of industrial butanol production from renewable feedstock, by acetone-butanol-ethanol (ABE) fermentation by clostridia, such as Clostridium beijerinckii, is the low final butanol concentration. ... -
Draft Genome Sequence of Clostridium pasteurianum NRRL B-598, a Potential Butanol or Hydrogen Producer
Kolek, Jan; Sedlář, Karel; Provazník, Ivo; Patáková, Petra (ASM, 2014-03-20)We present a draft genome sequence of Clostridium pasteurianum NRRL B-598. This strain ferments saccharides by two-stage acetone-butanol (AB) fermentation, is oxygen tolerant, and has high hydrogen yields. -
Identification and validation of reference genes in Clostridium beijerinckii NRRL B-598 for RT-qPCR using RNA-Seq data
Jurečková, Kateřina; Raschmanová, Hana; Kolek, Jan; Vasylkivska, Maryna; Branská, Barbora; Patáková, Petra; Provazník, Ivo; Sedlář, Karel (Frontiers, 2021-03-04)Gene expression analysis through reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) depends on correct data normalization by reference genes with stable expression. Although Clostridium ... -
Phenotypic and genomic analysis of Clostridium beijerinckii NRRL B-598 mutants with increased butanol tolerance
Vasylkivska, Maryna; Branská, Barbora; Sedlář, Karel; Jurečková, Kateřina; Provazník, Ivo; Patáková, Petra (Frontiers, 2020-11-05)N-Butanol, a valuable solvent and potential fuel extender, can be produced via acetone-butanol-ethanol (ABE) fermentation. One of the main drawbacks of ABE fermentation is the high toxicity of butanol to producing cells, ... -
Phenotypic and genomic analysis of isopropanol and 1,3-propanediol producer Clostridium diolis DSM 15410
Sedlář, Karel; Vasylkivska, Maryna; Musilová, Jana; Branská, Barbora; Provazník, Ivo; Patáková, Petra (Elsevier, 2020-11-07)Clostridium diolis DSM 15410 is a type strain of solventogenic clostridium capable of conducting isopropanol-butanol-ethanol fermentation. By studying its growth on different carbohydrates, we verified its ability to utilize ... -
Transcription profiling of butanol producer Clostridium beijerinckii NRRL B-598 using RNA-Seq
Sedlář, Karel; Cicková, Pavlína; Vasylkivska, Maryna; Branská, Barbora; Kolek, Jan; Kupková, Kristýna; Patáková, Petra; Provazník, Ivo (BioMed Central, 2018-05-30)Background Thinning supplies of natural resources increase attention to sustainable microbial production of bio-based fuels. The strain Clostridium beijerinckii NRRL B-598 is a relatively well-described butanol producer ... -
Transcriptional analysis of amino acid, metal ion, vitamin and carbohydrate uptake in butanol-producing Clostridium beijerinckii NRRL B-598
Vasylkivska, Maryna; Jurečková, Kateřina; Branská, Barbora; Sedlář, Karel; Kolek, Jan; Provazník, Ivo; Patáková, Petra (PLOS, 2019-11-07)In-depth knowledge of cell metabolism and nutrient uptake mechanisms can lead to the development of a tool for improving acetone-butanol-ethanol (ABE) fermentation performance and help to overcome bottlenecks in the process, ... -
A transcriptional response of Clostridium beijerinckii NRRL B-598 to a butanol shock
Sedlář, Karel; Kolek, Jan; Gruber, Markus; Jurečková, Kateřina; Branská, Barbora; Csaba, Gergely; Vasylkivska, Maryna; Zimmer, Ralf; Patáková, Petra; Provazník, Ivo (BioMed Central, 2019-10-13)Background One of the main obstacles preventing solventogenic clostridia from achieving higher yields in biofuel production is the toxicity of produced solvents. Unfortunately, regulatory mechanisms responsible for the ...