Transfection is based on nonviral delivery of nucleic acids or proteins into cells. Viral approaches are being used; nevertheless, their translational capacity is nowadays decreasing due to persistent fear of their safety, ...

Here, using human metallothionein (MT2) as an example, we describe an improved strategy based on differential alkylation coupled to MS, assisted by zinc probe monitoring, for identification of cysteine-rich binding sites ...

Mammalian metallothioneins (MTs) are a group of cysteine-rich proteins that bind metal ions in two alpha- and beta-domains and represent a major cellular Zn(II)/Cu(I) buffering system in the cell. At cellular free Zn(II) ...

Metallothioneins (MT) are low molecular weight, cysteine-rich proteins maintaining metal ions homeostasis. They play a role in carcinogenesis and may also cause chemoresistance. The aim of the study was to explore the ...

Volné radikály jsou chemické částice, které obsahují jeden nebo více nepárových elektronů, které mohou být součástí molekuly. Způsobují, že se molekula stává velmi reaktivní. Volné radikály jsou také známo, že hrají dvojí ...

Human metallothionein-3 (hMT-3), also known as growth inhibitory factor, is predominantly expressed in the central nervous system. hMT-3 is presumed to participate in the processes of heavy metal detoxification, regulation ...

Background. Metallothioneins (MTs) constitute a family of evolutionary conserved low molecular weight proteins with small variations in their amino acid sequences. They play a role in the regulation of trace metals metabolism, ...

Taurine exhibits many positive effects on organism, such as induction of important protective biochemical mechanisms and antioxidant effects. In this work, we studied the effect of taurine consumption on levels of taurine, ...