Chytré nanonástroje
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The application of capillary electrophoresis, mass spectrometry and Brdicka reaction in human and rabbit metallothioneins analysis
(Wroclaw Medical University, 2018-11-30)Background. Metallothioneins (MTs) constitute a family of evolutionary conserved low molecular weight proteins with small variations in their amino acid sequences. They play a role in the regulation of trace metals metabolism, ... -
Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals
(MDPI, 2019-07-03)The inhibition effect of the selected heavy metals (Ag+, Cd2+, Cu2+, and Hg2+) on glucose oxidase (GOx) enzyme from Aspergillus niger (EC 1.1.3.4.) was studied using a new amperometric biosensor with an electrochemical ... -
Electrochemical evaluation of selenium (IV) removal from its aqueous solutions by unmodified and modified graphene oxide
(MDPI, 2019-03-02)The removal of selenium from superficial and waste water is a worldwide problem. The maximum limit according to the World Health Organization (WHO) for the selenium in the water is set at a concentration of 10 g/L. Carbon ... -
Transcriptomic landscape of cisplatin-resistant neuroblastoma cells
(MDPI, 2019-03-31)The efficiency of cisplatin (CDDP) is significantly hindered by the development of resistance during the treatment course. To gain a detailed understanding of the molecular mechanisms underlying the development of cisplatin ... -
Phenolic profile of edible honeysuckle berries (Genus Lonicera) and their biological effect
(MDPI, 2012-01-01)The current status of research on polyphenolic compounds in the berries of edible honeysuckle and their biological effects, including recommended utilization, are reviewed. The major classes of phenolic compounds in the ... -
Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers
(MDPI, 2019-05-31)Despite distinctive advances in the field of head and neck squamous cell cancer (HNSCC) biomarker discovery, the spectrum of clinically useful prognostic serum biomarkers is limited. As metabolic activities in highly ... -
Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots
(MDPI, 2013-12-13)In this study we describe a beads-based assay for rapid, sensitive and specific isolation and detection of influenza vaccine hemagglutinin (HA). Amplification of the hemagglutinin signal resulted from binding of an ... -
Capillary Blood GSH Level Monitoring, Using an Electrochemical Method Adapted for Micro Volumes
(MDPI, 2018-10-01)Glutathione (gamma-glutamyl-cysteinyl-glycine; also known as GSH) is an endogenous antioxidant that plays a crucial role in cell defense mechanisms against oxidative stress. It is thus not surprising that this molecule can ... -
Raman and XPS studies of ammonia sensitive polypyrrole nanorods and nanoparticles
(Springer Nature, 2019-06-11)Raman and XPS studies of ammonia sensitive polypyrrole nanorods and nanoparticles, respectively and tested upon ammonia exposure using Raman and X-ray photonelectrone spectroscopy (XPS). Characterization of both nanomaterilas ... -
Isolation of Arginine Using Maghemite Particles
(TANGER, 2019-02-01)In this paper, we report the optimal conditions for the isolation of amino acid arginine from different types of matrix, such as blood, plasma or tissue and their characterization using ion-exchange liquid chromatography with ... -
-Fe2O3 Nanoparticles as a Potential Sensor for Histamine Isolation
(TANGER, 2019-02-01)Histamine, biologically active amine, is normally present in the body and it is involved in a local regulation of physiological processes. It occurs in food as a product of microbial decarboxylation of amino acid histidine, and ... -
The Effect of Temperature Treatment TiO2 Nanoparticles on Antibacterial Properties
(TANGER, 2019-02-01)The synthesis of TiO2 nanoparticles (NPs) under various temperature treatments was described and TiO2 NPs was subsequently tested as an antibacterial agent. Scanning electron microscopy (SEM), Raman spectroscopy, Dynamic ... -
Investigating the interplay between sarcosine and Ca2+-dependent signaling in prostate cells
(Mendel University in Brno, 2019-01-02)It has been shown that sarcosine supplementation stimulates proliferation and invassivenes of prostate cells. Nevertheless, the exact molecular mechanism responsible for this phenomenon is not known. In the present study ... -
Evaluation of cytotoxicity of biphasic TiO2 nanoparticles with organic surface coatings
(Mendel University in Brno, 2019-01-02)Titanium dioxide nanoparticles (TiO2 NPs) are used in lots of human applications because of their extraordinary nano scaled properties. Particularly, due to their photoprotective properties, they are used in topical ... -
Modification of electrothermal atomic absorption spectrometry for determination of arsenic in high salinity samples
(Mendel University in Brno, 2019-01-02)The electrothermal atomic absorption spectrometry (ET-AAS) method was optimized for determination of the arsenic content in high salinity samples. The combination of palladium (1 g/l) modifier, graphite furnace temperature ... -
Effect of apoferritin surface-biomacromolecular modification on cellular uptake and inhibition of protein corona
(Mendel University in Brno, 2019-01-02)The effects of surface modifications (PEGylation and PASylation) of natural nanocarriers based on apoferritin (FRT) were tested in this work. The main goals of performed PEGylation/PASylation were decreased protein corona ... -
Magnetic Molecularly Imprinted Polymers Used for Staphylococcus Aureus Isolation and Detection
(TANGER, 2019-02-01)A sensitive detection of microorganisms plays an important role in prevention and identification of infectious and deadly diseases. However, conventional methods of bacterial detection are time-consuming, laborious ... -
Utilization of antibody-nanoparticle conjugates as a tool for immunochemistry with ICP-MS detection
(Mendel University in Brno, 2019-01-02)Immunoanalytical techniques are key methods of application in clinical diagnostics, genomics, proteomics and other biochemical and molecular biology disciplines. Most often, they are based on the ability of labeled antibodies ... -
MALDI-TOF MSI method for determining spatial distribution of infection markers in pulmonary tissues of pigs
(Mendel University in Brno, 2019-01-02)In recent years the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI-TOF MSI) is used for molecular mapping of diverse biomarkers such as proteins or peptides in animal/plant ... -
Preparation of cryosections from frozen porcine pulmonary tissue for MALDI mass spectrometry imaging
(Mendel University in Brno, 2019-01-02)In last decades, a matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) was used for mapping the spatial distribution of different molecules, mainly peptides, proteins, lipids and metabolites, ...
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