Chytré nanonástroje
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A chemometric-assisted voltammetric analysis of free and Zn(II)-loaded metallothionein-3 states
(Elsevier, 2020-08-31)We focused on the application of mass spectrometry and electrochemical methods combined with a chemometric analysis for the characterization of partially Zn(II)-loaded metallothionein-3 species. The results showed decreased ... -
Hippocampal Neurons’ Alignment on Quartz Grooves and Parylene Cues on Quartz Substrate
(MDPI, 2020-12-30)Alignment and patterning of neurons have great importance in some research fields, especially for regenerative medicine and for the formation of artificial neural networks. Alignment of neurons on quartz grooves and parylene ... -
Antibacterial activity of AgNPs-TiO2 nanotubes: influence of different nanoparticle stabilizers
(Royal Society of Chemistry, 2020-12-15)Enhanced antibacterial properties of nanomaterials such as TiO2 nanotubes (TNTs) and silver nanoparticles (AgNPs) have attracted much attention in biomedicine and industry. The antibacterial properties of nanoparticles ... -
AACVD Synthesis and Characterization of Iron and Copper Oxides Modified ZnO Structured Films
(MDPI, 2020-03-05)Non-modified (ZnO) and modified (Fe2O3@ZnO and CuO@ZnO) structured films are deposited via aerosol assisted chemical vapor deposition. The surface modification of ZnO with iron or copper oxides is achieved in a second ... -
Synergistic Effect of Chitosan and Selenium Nanoparticles on Biodegradation and Antibacterial Properties of Collagenous Scaffolds Designed for Infected Burn Wounds
(MDPI, 2020-10-05)A highly porous scaffold is a desirable outcome in the field of tissue engineering. The porous structure mediates water-retaining properties that ensure good nutrient transportation as well as creates a suitable environment ... -
Comparison of dietary oils with different polyunsaturated fatty acid n-3 and n-6 content in the rat model of cutaneous wound healing
(MDPI, 2020-10-24)Dietary supplementation with polyunsaturated fatty acids (PUFA) n-3 can affect cutaneous wound healing; however, recent findings demonstrate the variable extent of their influence on the quality of healing. Here, we compare ... -
The effect of synthesis procedure on hydrogen peroxidase-like catalytic activity of iron oxide magnetic particles
(MDPI, 2020-09-27)A comparative study was carried out using magnetic nanoparticles (MNPs) for the fabrication of non-enzymatic sensors for the continuous and rapid detection and monitoring of H2O2. Various MNPs, differing in terms of their ... -
Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensing
(Elsevier, 2019-06-01)We report a facile method for detection of metallothionein (MT), a promising clinically relevant biomarker, in spiked plasma samples. This method, for the first time, integrates molecularly imprinted polymers as ... -
Hydroxyproline assay by HPLC-FLD applied for wound healing determination in rat model
(Mendel University in Brno, 2019-12-31)In this work, we focused on four types of oils and their response on wound healing of the skin of rats after dietary exposure. A set of samples consisted of 48 adult male rats of Wistar albino, which were divided into four ... -
Love Wave Sensors with Silver Modified Polypyrrole Nanoparticles for VOCs Monitoring
(MDPI, 2020-03-06)Love wave sensors with silver-modified polypyrrole nanoparticles are developed in this work. These systems prove functional at room temperature with enhanced response, sensitivity and response time, as compared to other ... -
Metallothionein Isoforms as Double Agents - Their Roles in Carcinogenesis, Cancer Progression and Chemoresistance
(Elsevier, 2020-09-30)Metallothioneins (MTs) are small cysteine-rich intracellular proteins with four major isoforms identified in mammals, designated MT-1 through MT-4. The best known biological functions of MTs are their ability to bind and ... -
Mass Spectrometry-Based Structural Analysis of Cysteine-Rich Metal-Binding Sites in Proteins with MetaOdysseus R Software
Identification of metal-binding sites in proteins and understanding metalcoupled protein folding mechanisms are aspects of high importance for the structure-tofunction relationship. Mass spectrometry (MS) has brought a ... -
Metal- and Affinity-Specific Dual Labeling of Cysteine-Rich Proteins for Identification of Metal-Binding Sites
(American Chemical Society, 2020-08-03)Here, using human metallothionein (MT2) as an example, we describe an improved strategy based on differential alkylation coupled to MS, assisted by zinc probe monitoring, for identification of cysteine-rich binding sites ... -
Proteomic signature of neuroblastoma cells UKF-NB-4 reveals key role of lysosomal sequestration and the proteasome complex in acquiring chemoresistance to cisplatin
(American Chemical Society, 2019-03-25)Cisplatin (CDDP) is a widely used agent in the treatment of neuroblastoma. Unfortunately, the development of acquired chemoresistance limits its clinical use. To gain a detailed understanding of the mechanisms underlying ... -
Rotamer Dynamics: Analysis of Rotamers in Molecular Dynamics Simulations of Proteins
(Elsevier, 2019-06-04)Given by chi torsional angles, rotamers describe the side-chain conformations of amino acid residues in a protein based on the rotational isomers (hence the word rotamer). Constructed rotamer libraries, based on either ... -
Norepinephrine Transporter-Derived Homing Peptides Enable Rapid Endocytosis of Drug Delivery Nanovehicles into Neuroblastoma Cells
(BioMed Central, 2020-07-13)Background: Currently, the diagnosis and treatment of neuroblastomas-the most frequent solid tumors in children-exploit the norepinephrine transporter (hNET) via radiolabeled norepinephrine analogs. We aim to develop a ... -
Drug Sequestration in Lysosomes as One of the Mechanisms of Chemoresistance of Cancer Cells and the Possibilities of Its Inhibition
(MDPI, 2020-06-30)Resistance to chemotherapeutics and targeted drugs is one of the main problems in successful cancer therapy. Various mechanisms have been identified to contribute to drug resistance. One of those mechanisms is lysosome-mediated ... -
UV-Induced Nanoparticles-Formation, Properties and Their Potential Role in Origin of Life
(MDPI, 2020-08-04)Inorganic nanoparticles might have played a vital role in the transition from inorganic chemistry to self-sustaining living systems. Such transition may have been triggered or controlled by processes requiring not only ... -
Direct fluorogenic detection of palladium and platinum organometallic complexes with proteins and nucleic acids in polyacrylamide gels
(Springer Nature, 2020-07-23)Allyl- and propargyl ethers of umbelliferone are sensitive probes for palladium and platinum, including anticancer compounds cisplatin, carboplatin and oxaliplatin, and effective for direct visualization of protein and DNA ... -
Effects of Cryopreservation on Cell Metabolic Activity and Function of Biofabricated Structures Laden with Osteoblasts
(MDPI, 2020-05-01)Biofabrication and maturation of bone constructs is a long-term task that requires a high degree of specialization. This specialization falls onto the hierarchy complexity of the bone tissue that limits the transfer of ...
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