Now showing items 98-117 of 142

  • On-chip three-dimensional cell culture in phaseguides improves hepatocyte functions in vitro 

    Jang, Mi; Neužil, Pavel; Volk, Thomas; Manz, Andreas; Kleber, Astrid (AIP Publishing, 2015-06-23)
    The in vitro study of liver functions and liver cell specific responses to external stimuli deals with the problem to preserve the in vivo functions of primary hepatocytes. In this study, we used the biochip OrganoPlateTM ...
  • Optimalization of DNA isolation process in freshwater microalgae using homogenizer 

    Bačová, Romana; Koláčková, Martina; Klejdus, Bořivoj; Húska, Dalibor (Mendel University in Brno, 2017-12-31)
    Last decades microalgae are gaining much interest due to high-valued biomolecules content. Increasing number of genetic studies and modifications require quality input material and short time analysis at the same time. ...
  • Oxidative Stress Resistance in Metastatic Prostate Cancer: Renewal by Self-Eating 

    Balvan, Jan; Gumulec, Jaromír; Raudenská, Martina; Křížová, Aneta; Štěpka, Petr; Babula, Petr; Kizek, René; Adam, Vojtěch; Masařík, Michal (PLOS, 2015-12-15)
    Resistant cancer phenotype is a key obstacle in the successful therapy of prostate cancer. The primary aim of our study was to explore resistance mechanisms in the advanced type of prostate cancer cells (PC-3) and to clarify ...
  • Papillomavirus infection of roe deer in the Czech Republic and fibropapilloma-associated levels of metallothionein, zinc, and oxidative stress 

    Král, Jiří; Banďouchová, Hana; Brichta, Jiří; Kovacova, Veronika; Ondráček, Karel; Osičková, Jitka; Hrubá, Hana; Hutařová, Zdeňka; Komínková, Markéta; Cernei, Natalia Vladimirovna; Konečná, Marie; Tmejová, Kateřina; Zítka, Ondřej; Adam, Vojtěch; Kízek, René; Skoric, Misa; Tremlová, Bohuslava; Treml, František; Pikula, Jiří (University of Veterinary and Pharmaceutical Sciences, 2015-06-01)
    The present study aimed at the aetiological diagnosis of skin tumours of roe deer (Capreolus capreolus) in the Czech Republic. A total of 33 roe deer specimens showing skin masses were sampled for histopathology, virus ...
  • Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection 

    Heger, Zbyněk; Cernei, Natalia Vladimirovna; Křížková, Soňa; Masařík, Michal; Kopel, Pavel; Hodek, Petr; Zítka, Ondřej; Adam, Vojtěch; Kizek, René (Nature Publishing Group, 2015-03-09)
    Herein, we describe an ultrasensitive specific biosensing system for detection of sarcosine as a potential biomarker of prostate carcinoma based on Forster resonance energy transfer (FRET). The FRET biosensor employs ...
  • Perspective of Use of Antiviral Peptides against Influenza Virus 

    Skaličková, Sylvie; Heger, Zbyněk; Krejčová, Ludmila; Pekařík, Vladimír; Bastl, Karel; Janda, Jozef; Kostolanský, František; Vařečková, Eva; Zítka, Ondřej; Adam, Vojtěch; Kizek, René (MDPI, 2015-10-01)
    The threat of a worldwide influenza pandemic has greatly increased over the past decade with the emergence of highly virulent avian influenza strains. The increased frequency of drug-resistant influenza strains against ...
  • Phenolic profile of edible honeysuckle berries (Genus Lonicera) and their biological effect 

    Juříková, Tunde; Rop, Otakar; Mlček, Jiří; Sochor, Jiří; Balla, Štefan; Szekeres, Ladislav; Hegedusová, A.; Hubálek, Jaromír; Adam, Vojtěch; Kizek, René (MDPI, 2012-01-01)
    The current status of research on polyphenolic compounds in the berries of edible honeysuckle and their biological effects, including recommended utilization, are reviewed. The major classes of phenolic compounds in the ...
  • Platinum nanoparticles induce damage to DNA and inhibit DNA replication 

    Nejdl, Lukáš; Kudr, Jiří; Moulick, Amitava; Hegerová, Dagmar; Ruttkay-Nedecký, Branislav; Gumulec, Jaromír; Číhalová, Kristýna; Šmerková, Kristýna; Dostálová, Simona; Křížková, Soňa; Novotná, Marie; Kopel, Pavel; Adam, Vojtěch (PLOS, 2017-07-12)
    Sparsely tested group of platinum nanoparticles (PtNPs) may have a comparable effect as complex platinum compounds. The aim of this study was to observe the effect of PtNPs in in vitro amplification of DNA fragment of phage ...
  • Preparation and Characterisation of Highly Stable Iron Oxide Nanoparticles for Magnetic Resonance Imaging 

    Kovář, David; Hubálek, Jaromír; Fohlerová, Zdenka; Malá, Aneta; Kalina, Michal; Jiřík, Radovan; Hlaváček, Antonín; Slabý, Ondřej; Skládal, Petr; Farka, Zdeněk; Mlčochová, Jitka; Starčuk jr., Zenon (Hindawi Publishing Corporation, 2017-02-23)
    Magnetic nanoparticles produced using aqueous coprecipitation usually exhibit wide particle size distribution. Synthesis of small and uniform magnetic nanoparticles has been the subject of extensive research over recent ...
  • Preparation and Optimisation of Cross-Linked Enzyme Aggregates Using Native Isolate White Rot Fungi Trametes versicolor and Fomes fomentarius for the Decolourisation of Synthetic Dyes 

    Vršanská, Martina; Voběrková, Stanislava; Jimenez Jimenez, Ana Maria; Strmiska, Vladislav; Adam, Vojtěch (MDPI, 2018-01-01)
    The key to obtaining an optimum performance of an enzyme is often a question of devising a suitable enzyme and optimisation of conditions for its immobilization. In this study, laccases from the native isolates of white ...
  • Preparation of cryosections from frozen porcine pulmonary tissue for MALDI mass spectrometry imaging 

    Jarošová, Rea; Smolíková, Vendula; Dvořák, Marek; Guráň, Roman; Do, Tomáš; Ondráčková, Petra; Zítka, Ondřej; Sládek, Zbyšek (Mendel University in Brno, 2019-01-02)
    In last decades, a matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) was used for mapping the spatial distribution of different molecules, mainly peptides, proteins, lipids and metabolites, ...
  • Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers 

    Všianský, Vít; Svobodová, Markéta; Gumulec, Jaromír; Cernei, Natalia Vladimirovna; Štěrbová, Dagmar; Zítka, Ondřej; Kostřica, Rom; Smilek, Pavel; Plzák, Jan; Betka, Jan; Kalfert, David; Masařík, Michal; Raudenská, Martina (MDPI, 2019-05-31)
    Despite distinctive advances in the field of head and neck squamous cell cancer (HNSCC) biomarker discovery, the spectrum of clinically useful prognostic serum biomarkers is limited. As metabolic activities in highly ...
  • Prostate tumor attenuation in the nu/nu murine model due to antisarcosine antibodies in folate targeted liposomes 

    Heger, Zbyněk; Polanská, Hana; Merlos Rodrigo, Miguel Ángel; Guráň, Roman; Kulich, Pavel; Kopel, Pavel; Masařík, Michal; Eckschlager, Tomáš; Stiborová, Marie; Kizek, René; Adam, Vojtěch (Nature Publishing Group, 2016-09-20)
    Herein, we describe the preparation of liposomes with folate-targeting properties for the encapsulation of anti-sarcosine antibodies (antisarAbs@LIP) and sarcosine (sar@LIP). The competitive inhibitory effects of exogenously ...
  • Prostate-Specific Membrane Antigen-Targeted Site-Directed Antibody-Conjugated Apoferritin Nanovehicle Favorably Influences In Vivo Side Effects of Doxorubicin 

    Dostálová, Simona; Polanská, Hana; Svobodová, Markéta; Balvan, Jan; Kryštofová, Olga; Haddad, Yazan Abdulmajeed Eyadh; Křížková, Soňa; Masařík, Michal; Eckschlager, Tomáš; Stiborová, Marie; Heger, Zbyněk; Adam, Vojtěch (Springer Nature, 2018-06-11)
    Herein, we describe the in vivo effects of doxorubicin (DOX) encapsulated in ubiquitous protein apoferritin (APO) and its efficiency and safety in anti-tumor treatment. APODOX is both passively (through Enhanced Permeability ...
  • Raman and XPS studies of ammonia sensitive polypyrrole nanorods and nanoparticles 

    Šetka, Milena; Calavia, Raul; Vojkůvka, Lukáš; Llobet, Eduard; Drbohlavová, Jana; Vallejos Vargas, Stella (Springer Nature, 2019-06-11)
    Raman and XPS studies of ammonia sensitive polypyrrole nanorods and nanoparticles, respectively and tested upon ammonia exposure using Raman and X-ray photonelectrone spectroscopy (XPS). Characterization of both nanomaterilas ...
  • Resistive switching in TiO2 nanocolumn arrays electrochemically grown 

    Márik, Marian; Mozalev, Alexander; Hubálek, Jaromír; Bendová, Mária (IOP Publishing, 2017-04-21)
    Resistive switching in metal oxides, especially in TiO2, has been intensively investigated for potential application in non-volatile memory microdevices. As one of the working mechanisms, a conducting filament consisting ...
  • The Role of Metallothionein in Oxidative Stress 

    Ruttkay-Nedecký, Branislav; Nejdl, Lukáš; Gumulec, Jaromír; Zítka, Ondřej; Masařík, Michal; Eckschlager, Tomáš; Stiborová, Marie; Adam, Vojtěch; Kizek, René (MDPI, 2013-03-15)
    Free radicals are chemical particles containing one or more unpaired electrons, which may be part of the molecule. They cause the molecule to become highly reactive. The free radicals are also known to play a dual role in ...
  • Ruthenium-based core-shell nanoparticles with exceptional in vitro biocompatibility 

    Buchtelová, Hana; Strmiska, Vladislav; Dostálová, Simona; Michálek, Petr; Křížková, Soňa; Kopel, Pavel; Hynek, David; Richtera, Lukáš; Adam, Vojtěch; Heger, Zbyněk (Mendel University in Brno, 2017-12-31)
    The current study demonstrates design preparation and characterization of biocompatible hybrid ruthenium core-shell nanoparticles (RuNPs) coated with polyvinylpyrrolidone (PVP) and polyoxyethylene stearate (POES). The ...
  • Sarcosine as a potential prostate cancer biomarker – A review 

    Cernei, Natalia Vladimirovna; Heger, Zbyněk; Gumulec, Jaromír; Zítka, Ondřej; Masařík, Michal; Babula, Petr; Eckschlager, Tomáš; Stiborová, Marie; Kizek, René; Adam, Vojtěch (MDPI, 2013-07-04)
    Prostate cancer (CaP) is the most common type of tumour disease in men. Early diagnosis of cancer of the prostate is very important, because the sooner the cancer is detected, the better it is treated. According to that ...
  • Sarcosine degradation pathway is involved in the epigenetics of prostate cells 

    Strmiska, Vladislav; Michálek, Petr; Buchtelová, Hana; Lacková, Zuzana; Guráň, Roman; Křížková, Soňa; Pompeiano Vaníčková, Lucie; Zítka, Ondřej; Stiborová, Marie; Eckschlager, Tomáš; Adam, Vojtěch; Heger, Zbyněk (Mendel University in Brno, 2017-12-31)
    It has been shown that sarcosine suplementation stimulates the proliferation of prostate cells and also their invassiveness. In present study we show that enzymes conected with sarcosine conversion to glycine (sarcosine ...