Now showing items 112-131 of 148

  • Preparation and Optimisation of Cross-Linked Enzyme Aggregates Using Native Isolate White Rot Fungi Trametes versicolor and Fomes fomentarius for the Decolourisation of Synthetic Dyes 

    Vršanská, Martina; Voběrková, Stanislava; Jimenez Jimenez, Ana Maria; Strmiska, Vladislav; Adam, Vojtěch (MDPI, 2018-01-01)
    The key to obtaining an optimum performance of an enzyme is often a question of devising a suitable enzyme and optimisation of conditions for its immobilization. In this study, laccases from the native isolates of white ...
  • Preparation of cryosections from frozen porcine pulmonary tissue for MALDI mass spectrometry imaging 

    Jarošová, Rea; Smolíková, Vendula; Dvořák, Marek; Guráň, Roman; Do, Tomáš; Ondráčková, Petra; Zítka, Ondřej; Sládek, Zbyšek (Mendel University in Brno, 2019-01-02)
    In last decades, a matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) was used for mapping the spatial distribution of different molecules, mainly peptides, proteins, lipids and metabolites, ...
  • Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers 

    Všianský, Vít; Svobodová, Markéta; Gumulec, Jaromír; Cernei, Natalia Vladimirovna; Štěrbová, Dagmar; Zítka, Ondřej; Kostřica, Rom; Smilek, Pavel; Plzák, Jan; Betka, Jan; Kalfert, David; Masařík, Michal; Raudenská, Martina (MDPI, 2019-05-31)
    Despite distinctive advances in the field of head and neck squamous cell cancer (HNSCC) biomarker discovery, the spectrum of clinically useful prognostic serum biomarkers is limited. As metabolic activities in highly ...
  • Prostate tumor attenuation in the nu/nu murine model due to antisarcosine antibodies in folate targeted liposomes 

    Heger, Zbyněk; Polanská, Hana; Merlos Rodrigo, Miguel Ángel; Guráň, Roman; Kulich, Pavel; Kopel, Pavel; Masařík, Michal; Eckschlager, Tomáš; Stiborová, Marie; Kizek, René; Adam, Vojtěch (Nature Publishing Group, 2016-09-20)
    Herein, we describe the preparation of liposomes with folate-targeting properties for the encapsulation of anti-sarcosine antibodies (antisarAbs@LIP) and sarcosine (sar@LIP). The competitive inhibitory effects of exogenously ...
  • Prostate-Specific Membrane Antigen-Targeted Site-Directed Antibody-Conjugated Apoferritin Nanovehicle Favorably Influences In Vivo Side Effects of Doxorubicin 

    Dostálová, Simona; Polanská, Hana; Svobodová, Markéta; Balvan, Jan; Kryštofová, Olga; Haddad, Yazan Abdulmajeed Eyadh; Křížková, Soňa; Masařík, Michal; Eckschlager, Tomáš; Stiborová, Marie; Heger, Zbyněk; Adam, Vojtěch (Springer Nature, 2018-06-11)
    Herein, we describe the in vivo effects of doxorubicin (DOX) encapsulated in ubiquitous protein apoferritin (APO) and its efficiency and safety in anti-tumor treatment. APODOX is both passively (through Enhanced Permeability ...
  • Raman and XPS studies of ammonia sensitive polypyrrole nanorods and nanoparticles 

    Šetka, Milena; Calavia, Raul; Vojkůvka, Lukáš; Llobet, Eduard; Drbohlavová, Jana; Vallejos Vargas, Stella (Springer Nature, 2019-06-11)
    Raman and XPS studies of ammonia sensitive polypyrrole nanorods and nanoparticles, respectively and tested upon ammonia exposure using Raman and X-ray photonelectrone spectroscopy (XPS). Characterization of both nanomaterilas ...
  • Resistive switching in TiO2 nanocolumn arrays electrochemically grown 

    Márik, Marian; Mozalev, Alexander; Hubálek, Jaromír; Bendová, Mária (IOP Publishing, 2017-04-21)
    Resistive switching in metal oxides, especially in TiO2, has been intensively investigated for potential application in non-volatile memory microdevices. As one of the working mechanisms, a conducting filament consisting ...
  • The Role of Metallothionein in Oxidative Stress 

    Ruttkay-Nedecký, Branislav; Nejdl, Lukáš; Gumulec, Jaromír; Zítka, Ondřej; Masařík, Michal; Eckschlager, Tomáš; Stiborová, Marie; Adam, Vojtěch; Kizek, René (MDPI, 2013-03-15)
    Free radicals are chemical particles containing one or more unpaired electrons, which may be part of the molecule. They cause the molecule to become highly reactive. The free radicals are also known to play a dual role in ...
  • Ruthenium-based core-shell nanoparticles with exceptional in vitro biocompatibility 

    Buchtelová, Hana; Strmiska, Vladislav; Dostálová, Simona; Michálek, Petr; Křížková, Soňa; Kopel, Pavel; Hynek, David; Richtera, Lukáš; Adam, Vojtěch; Heger, Zbyněk (Mendel University in Brno, 2017-12-31)
    The current study demonstrates design preparation and characterization of biocompatible hybrid ruthenium core-shell nanoparticles (RuNPs) coated with polyvinylpyrrolidone (PVP) and polyoxyethylene stearate (POES). The ...
  • Sarcosine as a potential prostate cancer biomarker – A review 

    Cernei, Natalia Vladimirovna; Heger, Zbyněk; Gumulec, Jaromír; Zítka, Ondřej; Masařík, Michal; Babula, Petr; Eckschlager, Tomáš; Stiborová, Marie; Kizek, René; Adam, Vojtěch (MDPI, 2013-07-04)
    Prostate cancer (CaP) is the most common type of tumour disease in men. Early diagnosis of cancer of the prostate is very important, because the sooner the cancer is detected, the better it is treated. According to that ...
  • Sarcosine degradation pathway is involved in the epigenetics of prostate cells 

    Strmiska, Vladislav; Michálek, Petr; Buchtelová, Hana; Lacková, Zuzana; Guráň, Roman; Křížková, Soňa; Pompeiano Vaníčková, Lucie; Zítka, Ondřej; Stiborová, Marie; Eckschlager, Tomáš; Adam, Vojtěch; Heger, Zbyněk (Mendel University in Brno, 2017-12-31)
    It has been shown that sarcosine suplementation stimulates the proliferation of prostate cells and also their invassiveness. In present study we show that enzymes conected with sarcosine conversion to glycine (sarcosine ...
  • Sarcosine is a prostate epigenetic modifier that elicits aberrant methylation patterns through the SAMe-Dnmts axis 

    Strmiska, Vladislav; Michálek, Petr; Lacková, Zuzana; Guráň, Roman; Křížková, Soňa; Pompeiano Vaníčková, Lucie; Zítka, Ondřej; Stiborová, Marie; Eckschlager, Tomáš; Klejdus, Bořivoj; Pacík, Dalibor; Tvrdíková, Eliška; Keil, Claudia; Haase, Hajo; Adam, Vojtěch; Heger, Zbyněk (FEBS Press, 2019-05-31)
    DNA hypermethylation is one of the most common epigenetic modifications in prostate cancer (PCa). Several studies have delineated sarcosine as a PCa oncometabolite that increases the migration of malignant prostate cells ...
  • Sarcosine up-regulates expression of genes involved in cell cycle progression of metastatic models of prostate cancer 

    Heger, Zbyněk; Merlos Rodrigo, Miguel Ángel; Michálek, Petr; Polanská, Hana; Masařík, Michal; Vít, Vítězslav; Plevová, Mariana; Pacík, Dalibor; Eckschlager, Tomáš; Stiborová, Marie; Adam, Vojtěch (PLOS, 2016-11-08)
    The effects of sarcosine on the processes driving prostate cancer (PCa) development remain still unclear. Herein, we show that a supplementation of metastatic PCa cells (androgen independent PC-3 and androgen dependent ...
  • Selectively arranged single-wire based nanosensor array systems for gas monitoring 

    Chmela, Ondřej; Sadílek, Jakub; Somer, Jakub; Mohan, Rajneesh; Hubálek, Jaromír; Vallejos Vargas, Stella (Royal Society of Chemistry, 2018-05-01)
    Gas nanosensors, comprised of arrays of nanoelectrodes with finger-widths of ~100 nm developed by electron beam lithography and aerosol assisted chemical vapor deposited non-functionalized and Pt-functionalized tungsten ...
  • Self-Ordered TiO2 Quantum Dot Array Prepared via Anodic Oxidation 

    Drbohlavová, Jana; Hrdý, Radim; Salyk, Ota; Kizek, René; Hubálek, Jaromír (SpringerOpen, 2012-02-14)
    The paper brings the important information about synthesis of titania quantum dots in the form of sensor array which is suitable for various biomolecules (DNA, proteins) sensing in vitro. The usage of this fluorescence ...
  • Serum and Tissue Zinc in Epithelial Malignancies: A Meta-Analysis 

    Gumulec, Jaromír; Masařík, Michal; Adam, Vojtěch; Eckschlager, Tomáš; Provazník, Ivo; Kizek, René (PLOS, 2014-06-19)
    Background and Objectives: Current studies give us inconsistent results regarding the association of neoplasms and zinc(II) serum and tissues concentrations. The results of to-date studies using meta-analysis are summarized ...
  • Serum metallothioneins in childhood tumours – A potential prognostic marker 

    Kruseová, Jarmila; Hynek, David; Adam, Vojtěch; Kizek, René; Průša, Richard; Hraběta, Jan; Eckschlager, Tomáš (MDPI, 2013-06-06)
    Metallothioneins (MT) are low molecular weight, cysteine-rich proteins maintaining metal ions homeostasis. They play a role in carcinogenesis and may also cause chemoresistance. The aim of the study was to explore the ...
  • Sharka: The Past, The Present and The Future 

    Sochor, Jiří; Babula, Petr; Adam, Vojtěch; Krška, Boris; Kizek, René (MDPI, 2012-11-01)
    Members the Potyviridae family belong to a group of plant viruses that are causing devastating plant diseases with a significant impact on agronomy and economics. Plum pox virus (PPV), as a causative agent of sharka disease, ...
  • Simultaneous Automatic Electrochemical Detection of Zinc, Cadmium, Copper and Lead Ions in Environmental Samples Using a Thin-Film Mercury Electrode and an Artificial Neural Network 

    Kudr, Jiří; Nguyen, Hoai Viet; Gumulec, Jaromír; Nejdl, Lukáš; Blažková, Iva; Ruttkay-Nedecký, Branislav; Hynek, David; Kynický, Jindřich; Adam, Vojtěch; Kizek, René (MDPI, 2015-01-01)
    In this study a device for automatic electrochemical analysis was designed. A three electrodes detection system was attached to a positioning device, which enabled us to move the electrode system from one well to another ...
  • Single Fluorescence Channel-based Multiplex Detection of Avian Influenza Virus by Quantitative PCR with Intercalating Dye 

    Ahrberg, Christian D.; Manz, Andreas; Neužil, Pavel (Nature Publishing Group, 2015-06-19)
    Since its invention in 1985 the polymerase chain reaction (PCR) has become a well-established method for amplification and detection of segments of double-stranded DNA. Incorporation of fluorogenic probe or DNA intercalating ...