Now showing items 1-20 of 27

  • Local Treatment of Hand-Foot Syndrome with Uridine/Thymidine:In Vitro Appraisal on a Human Keratinocyte Cell Line HaCaT 

    Hartinger, Jan; Veselý, Pavel; Matoušková, Eva; Argalacsová, Soňa; Petruželka, Luboš; Netíková, Irena (Hindawi, 2012-07-31)
    5-fluorouracil (5-FU) is one of the most commonly used antineoplastic drugs in the anticancer therapy. The hand-foot (HF) syndrome (palmar-plantar erythrodysesthesia) is an adverse effect frequently related to long-term ...
  • Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis 

    Balvan, Jan; Křížová, Aneta; Gumulec, Jaromír; Raudenská, Martina; Sládek, Zbyšek; Sedláčková, Miroslava; Babula, Petr; Svobodová, Markéta; Kizek, René; Chmelík, Radim; Masařík, Michal (PLOS, 2015-03-24)
    Identification of specific cell death is of a great value for many scientists. Predominant types of cell death can be detected by flow-cytometry (FCM). Nevertheless, the absence of cellular morphology analysis leads to the ...
  • Automated alignment method for coherence-controlled holographic microscope 

    Dostál, Zbyněk; Slabý, Tomáš; Kvasnica, Lukáš; Lošťák, Martin; Křížová, Aneta; Chmelík, Radim (SPIE, 2015-10-28)
    Coherence-controlled holographic microscope (CCHM) was developed particularly for quantitative phase imaging and measurement of live cell dynamics, which is the proper subject of digital holographic microscopy (DHM). CCHM ...
  • Quantitative phase imaging through scattering media by means of coherence-controlled holographic microscope 

    Kollárová, Věra; Čolláková, Jana; Dostál, Zbyněk; Veselý, Pavel; Chmelík, Radim (SPIE, 2015-11-01)
    A coherence-controlled holographic microscope (CCHM) enables quantitative phase imaging with coherent as well as incoherent illumination. The low spatially coherent light induces a coherence gating effect, which makes ...
  • Coherence-controlled holographic microscopy enabled recognition of necrosis as the mechanism of cancer cells death after exposure to cytopathic turbid emulsion. 

    Čolláková, Jana; Křížová, Aneta; Kollárová, Věra; Dostál, Zbyněk; Slabá, Michala; Veselý, Pavel; Chmelík, Radim (Journal of Biomedical Optics SPIE, 2015-11-01)
    Coherence-controlled holographic microscopy (CCHM) in low-coherence mode possesses pronounced coherence gate effect. This offers an option to investigate the details of cellular events leading to cell death caused by ...
  • Multimode fibre: Light-sheet microscopy at the tip of a needle 

    Plöschner, Martin; Kollárová, Věra; Dostál, Zbyněk; Nylk, Jonathan; Barton-Owen, Thomas; Ferrier, David E.K.; Chmelík, Radim; Dholakia, Kishan; Čižmár, Tomáš (Springer Nature, 2015-12-14)
    Light-sheet fluorescence microscopy has emerged as a powerful platform for 3-D volumetric imaging in the life sciences. Here, we introduce an important step towards its use deep inside biological tissue. Our new technique, ...
  • Oxidative Stress Resistance in Metastatic Prostate Cancer: Renewal by Self-Eating 

    Balvan, Jan; Gumulec, Jaromír; Raudenská, Martina; Křížová, Aneta; Štěpka, Petr; Babula, Petr; Kizek, René; Adam, Vojtěch; Masařík, Michal (PLOS, 2015-12-15)
    Resistant cancer phenotype is a key obstacle in the successful therapy of prostate cancer. The primary aim of our study was to explore resistance mechanisms in the advanced type of prostate cancer cells (PC-3) and to clarify ...
  • Cell type specificity of neurovascular coupling in cerebral cortex 

    Uhlířová, Hana; Klç, Kvlcm; Tian, Pfeifang; Thunemann, Martin; Desjardins, Michele; Saisan, Payam A.; Sakadžic, Sava; Ness, Torbjrn V; Mateo, Celine; Cheng, Qun; Weldy, Kimberly L.; Razoux, Florence; Vandenberghe, Matthieu; Cremonesi, Jonathan A; Ferri, Christopher GL; Nizar, Krystal; Sridhar, Vishnu B.; Steed, Tyler C.; Abashin, Maxim; Fainman, Yeshaiahu; Masliah, Eliezer; Djurovic, Srdjan; Andreassen, Ola A; Silva, Gabriel A.; Boas, David A.; Kleinfeld, David; Buxton, Richard B; Einevoll, Gaute T.; Dale, Anders M.; Devor, Anna (eLife Sciences Publications, 2016-05-31)
    Identification of the cellular players and molecular messengers that communicate neuronal activity to the vasculature driving cerebral hemodynamics is important for (1) the basic understanding of cerebrovascular regulation ...
  • The adhesion of normal human dermal fibroblasts to the cyclopropylamine plasma polymers studied by holographic microscopy 

    Štrbková, Lenka; Manakhov, Anton; Zajíčková, Lenka; Stoica, Adrian; Veselý, Pavel; Chmelík, Radim (Elsevier B.V., 2016-06-15)
    The understanding of cell–surface interactions plays an important role for the biomaterials development and bioengineering. Although it is already known that amine groups increase the cell adhesion and proliferation, the ...
  • Chk1 inhibition significantly potentiates activity of nucleoside analogs in TP53-mutated B-lymphoid cells 

    Zemanová, Jana; Hylse, Ondřej; Čolláková, Jana; Veselý, Pavel; Oltová, Alexandra; Borský, Marek; Zápražná, Kristína; Kašpárková, Marie; Janovská, Pavlína; Verner, Jan; Kohoutek, Jiří; Dzimková, Marta; Bryja, Vítězslav; Jašková, Zuzana; Brychtová, Yvona; Paruch, Kamil; Trbušek, Martin (Impact Journals, 2016-08-19)
    Treatment options for TP53-mutated lymphoid tumors are very limited. In experimental models, TP53-mutated lymphomas were sensitive to direct inhibition of checkpoint kinase 1 (Chk1), a pivotal regulator of replication. We ...
  • Neurovascular Network Explorer 2.0: A Database of 2-Photon Single Vessel Diameter Measurements from Mouse SI Cortexin Response To Optogenetic Stimulation 

    Uhlířová, Hana; Peifang, Tiam; Kilic, Kivilcim; Thunemann, Martin; Sridhar, Vishnu B.; Bartsch, Hauke; Dale, Anders M.; Devor, Anna; Saisan, Payam A. (Frontiers, 2017-02-01)
    Here, we present a novel database containing 2-photon data from our recently published experimental study (Uhlirova et al., 2016a) and a second generation of our GUI-based software engine that we call NNE 2.0. The data, ...
  • Migrastatics-Anti-metastatic and Anti-invasion Drugs: Promises and Challenges 

    Gandalovičová, Aneta; Rösel, Daniel; Fernandes, Michael; Veselý, Pavel; Henenberg, Petr; Čermák, Vladimír; Petruželka, Luboš; Kumar, Sunil; Sanz-Moreno, Victoria; Brábek, Jan (Elsevier, 2017-06-01)
    In solid cancers, invasion and metastasis account for more than 90% of mortality. However, in the current armory of anticancer therapies, a specific category of anti-invasion and antimetastatic drugs is missing. Here, we ...
  • Automated classification of cell morphology by coherence-controlled holographic microscopy 

    Štrbková, Lenka; Zicha, Daniel; Veselý, Pavel; Chmelík, Radim (SPIE, 2017-08-23)
    In the last few years, classification of cells by machine learning has become frequently used in biology. However, most of the approaches are based on morphometric (MO) features, which are not quantitative in terms of cell ...
  • Distinctive behaviour of live biopsy-derived carcinoma cells unveiled using coherence-controlled holographic microscopy 

    Gál, Břetislav; Veselý, Miroslav; Čolláková, Jana; Nekulová, Marta; Jůzová, Veronika; Chmelík, Radim; Veselý, Pavel (PLOS, 2017-08-28)
    Head and neck squamous cell carcinoma is one of the most aggressive tumours and is typically diagnosed too late. Late diagnosis requires an urgent decision on an effective therapy. An individualized test of chemosensitivity ...
  • Phasor analysis of NADH FLIM identifies pharmacological disruptions to mitochondrial metabolic processes in the rodent cerebral cortex 

    Gómez, Carlos A.; Sutin, Jason; Wu, Weicheng; Fu, Buyin; Uhlířová, Hana; Devor, Anna; Boas, David A.; Sakadžic, Sava; Yaseen, Mohammad Abbas (PLOS, 2018-03-21)
    Investigating cerebral metabolism in vivo at a microscopic level is essential for understanding brain function and its pathological alterations. The intricate signaling and metabolic dynamics between neurons, glia, and ...
  • Quantitative phase imaging unravels new insight into dynamics of mesenchymal and amoeboid cancer cell invasion 

    Tolde, Ondřej; Gandalovičová, Aneta; Křížová, Aneta; Veselý, Pavel; Chmelík, Radim; Rösel, Daniel; Brábek, Jan (Springer Nature, 2018-08-13)
    Observation and analysis of cancer cell behaviour in 3D environment is essential for full understanding of the mechanisms of cancer cell invasion. However, label-free imaging of live cells in 3D conditions is optically ...
  • High-Resolution Quantitative Phase Imaging of Plasmonic Metasurfaces with Sensitivity down to a Single Nanoantenna 

    Bouchal, Petr; Dvořák, Petr; Babocký, Jiří; Bouchal, Zdeněk; Ligmajer, Filip; Hrtoň, Martin; Křápek, Vlastimil; Faßbender, Alexander; Linden, Stefan; Chmelík, Radim; Šikola, Tomáš (American Chemical Society, 2019-01-02)
    Optical metasurfaces have emerged as a new generation of building blocks for multifunctional optics. Design and realization of metasurface elements place everincreasing demands on accurate assessment of phase alterations ...
  • Geometric-Phase Microscopy for Quantitative Phase Imaging of Isotropic, Birefringent and Space-Variant Polarization Samples 

    Bouchal, Petr; Štrbková, Lenka; Dostál, Zbyněk; Chmelík, Radim; Bouchal, Zdeněk (Springer Nature, 2019-03-05)
    We present geometric-phase microscopy allowing a multipurpose quantitative phase imaging in which the ground-truth phase is restored by quantifying the phase retardance. The method uses broadband spatially incoherent light ...
  • Dual Targeting of BRAF and mTOR Signaling in Melanoma Cells with Pyridinyl Imidazole Compounds 

    Palušová, Veronika; Renzová, Tereza; Verlande, Amandine; Vaclová, Tereza; Medková, Michaela; Cetlová, Linda; Sedláčková, Miroslava; Hříbková, Hana; Slaninová, Iva; Krutá, Miriama; Rotrekl, Vladimír; Uhlířová, Hana; Křížová, Aneta; Chmelík, Radim; Veselý, Pavel; Krafčíková, Michaela; Trantírek, Lukáš; Schink, Kay Oliver; Uldrijan, Stjepan (MDPI, 2020-06-01)
    BRAF inhibitors can delay the progression of metastatic melanoma, but resistance usually emerges, leading to relapse. Drugs simultaneously targeting two or more pathways essential for cancer growth could slow or prevent ...
  • Silicon waveguides with graphene: coupling of waveguide mode to surface plasmons 

    Čtyroký, Jiří; Petráček, Jiří; Kuzmiak, Vladimír; Kwiecien, Pavel; Richter, Ivan (IOP Publishing, 2020-08-18)
    Silicon waveguides with graphene layers have been recently intensively studied for their potential as fast and low-power electro-optic modulators with small footprints. In this paper we show that in the optical wavelength ...