Browsing Experimentální biofotonika by Title
Now showing items 1-20 of 33
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Addressing cancer invasion and cell motility with quantitative light microscopy
(NATURE PORTFOLIO, 2022-01-10)The incidence of death caused by cancer has been increasing worldwide. The growth of cancer cells is not the main problem. The majority of deaths are due to invasion and metastasis, where cancer cells actively spread from ... -
The adhesion of normal human dermal fibroblasts to the cyclopropylamine plasma polymers studied by holographic microscopy
(Elsevier B.V., 2016-06-15)The understanding of cell–surface interactions plays an important role for the biomaterials development and bioengineering. Although it is already known that amine groups increase the cell adhesion and proliferation, the ... -
Advanced Microscopical Non-Invasive Examination of the Supposed Migrastatics for Impact on In Vitro Cell Migration
(European Conference of Oncology Pharmacy, 2022-07-02)Live H1299 lung carcinoma cells in vitro were exposed to selected drugs with a presumed antimigration activity that implies antimetastatic potential and time-lapse examined with Coherence Controlled Holography Microscopy ... -
Automated alignment method for coherence-controlled holographic microscope
(SPIE, 2015-10-28)Coherence-controlled holographic microscope (CCHM) was developed particularly for quantitative phase imaging and measurement of live cell dynamics, which is the proper subject of digital holographic microscopy (DHM). CCHM ... -
Automated classification of cell morphology by coherence-controlled holographic microscopy
(SPIE, 2017-08-23)In the last few years, classification of cells by machine learning has become frequently used in biology. However, most of the approaches are based on morphometric (MO) features, which are not quantitative in terms of cell ... -
Automated interpretation of time-lapse quantitative phase image by machine learning to study cellular dynamics during epithelial-mesenchymal transition
(SPIE, 2020-08-31)Significance: Machine learning is increasingly being applied to the classification of microscopic data. In order to detect some complex and dynamic cellular processes, time-resolved live-cell imaging might be necessary. ... -
Cell type specificity of neurovascular coupling in cerebral cortex
(eLife Sciences Publications, 2016-05-31)Identification of the cellular players and molecular messengers that communicate neuronal activity to the vasculature driving cerebral hemodynamics is important for (1) the basic understanding of cerebrovascular regulation ... -
Chk1 inhibition significantly potentiates activity of nucleoside analogs in TP53-mutated B-lymphoid cells
(Impact Journals, 2016-08-19)Treatment options for TP53-mutated lymphoid tumors are very limited. In experimental models, TP53-mutated lymphomas were sensitive to direct inhibition of checkpoint kinase 1 (Chk1), a pivotal regulator of replication. We ... -
Coherence gate manipulation for enhanced imaging through scattering media by non-ballistic light in partially coherent interferometric systems
(The Optical Society, 2021-09-15)Coherence gating is typically exploited for imaging through disordered media by least-scattered (ballistic) light. Ballistic light-based approaches produce clear images only when the proportion of ballistic to multiply ... -
Coherence-controlled holographic microscopy enabled recognition of necrosis as the mechanism of cancer cells death after exposure to cytopathic turbid emulsion.
(Journal of Biomedical Optics SPIE, 2015-11-01)Coherence-controlled holographic microscopy (CCHM) in low-coherence mode possesses pronounced coherence gate effect. This offers an option to investigate the details of cellular events leading to cell death caused by ... -
Coherence-encoded synthetic aperture for super-resolution quantitative phase imaging
(AIP Publishing, 2022-04-01)Quantitative phase imaging (QPI) has quickly established its role in identifying rare events and screening in biomedicine or automated image data analysis using artificial intelligence. These and many other applications ... -
Computational image enhancement of multimode fibre-based holographic endo-microscopy: harnessing the muddy modes
(Optica Publishing Group, 2021-11-08)In imaging geometries, which employ wavefront-shaping to control the light transport through a multi-mode optical fibre (MMF), this terminal hair-thin optical component acts as a minimally invasive objective lens, enabling ... -
Distinctive behaviour of live biopsy-derived carcinoma cells unveiled using coherence-controlled holographic microscopy
(PLOS, 2017-08-28)Head and neck squamous cell carcinoma is one of the most aggressive tumours and is typically diagnosed too late. Late diagnosis requires an urgent decision on an effective therapy. An individualized test of chemosensitivity ... -
Dual Targeting of BRAF and mTOR Signaling in Melanoma Cells with Pyridinyl Imidazole Compounds
(MDPI, 2020-06-01)BRAF inhibitors can delay the progression of metastatic melanoma, but resistance usually emerges, leading to relapse. Drugs simultaneously targeting two or more pathways essential for cancer growth could slow or prevent ... -
Geometric-Phase Microscopy for Quantitative Phase Imaging of Isotropic, Birefringent and Space-Variant Polarization Samples
(Springer Nature, 2019-03-05)We present geometric-phase microscopy allowing a multipurpose quantitative phase imaging in which the ground-truth phase is restored by quantifying the phase retardance. The method uses broadband spatially incoherent light ... -
High-Resolution Quantitative Phase Imaging of Plasmonic Metasurfaces with Sensitivity down to a Single Nanoantenna
(American Chemical Society, 2019-01-02)Optical metasurfaces have emerged as a new generation of building blocks for multifunctional optics. Design and realization of metasurface elements place everincreasing demands on accurate assessment of phase alterations ... -
Lens stars and Platonic lenses
(Optical Society of America, 2021-12-06)Lens stars comprise identical ideal thin lenses arranged in a regular star shape centred on the common principal point. They satisfy the edge-imaging condition of transformation optics (TO) and are thus suitable as building ... -
Local Treatment of Hand-Foot Syndrome with Uridine/Thymidine:In Vitro Appraisal on a Human Keratinocyte Cell Line HaCaT
(Hindawi, 2012-07-31)5-fluorouracil (5-FU) is one of the most commonly used antineoplastic drugs in the anticancer therapy. The hand-foot (HF) syndrome (palmar-plantar erythrodysesthesia) is an adverse effect frequently related to long-term ... -
Migrastatics-Anti-metastatic and Anti-invasion Drugs: Promises and Challenges
(Elsevier, 2017-06-01)In solid cancers, invasion and metastasis account for more than 90% of mortality. However, in the current armory of anticancer therapies, a specific category of anti-invasion and antimetastatic drugs is missing. Here, we ... -
Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis
(PLOS, 2015-03-24)Identification of specific cell death is of a great value for many scientists. Predominant types of cell death can be detected by flow-cytometry (FCM). Nevertheless, the absence of cellular morphology analysis leads to the ...