Ústav chemie potravin a biotechnologií
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Evaluating the Influence of a G-Quadruplex Prone Sequence on the Transactivation Potential by Wild-Type and/or Mutant P53 Family Proteins through a Yeast-Based Functional Assay
(MDPI, 2021-02-15)P53, P63, and P73 proteins belong to the P53 family of transcription factors, sharing a common gene organization that, from the P1 and P2 promoters, produces two groups of mRNAs encoding proteins with different N-terminal ... -
The Use of Raman Spectroscopy to Monitor Metabolic Changes in Stressed Metschnikowia sp. Yeasts
(MDPI, 2021-01-29)Raman spectroscopy is a universal method designed for the analysis of a wide range of physical, chemical and biological systems or various surfaces. This technique is suitable to monitor various components of cells, tissues ... -
Simultaneous determination of sweeteners and preservatives in beverages by HPLC-DAD-ELSD
(HACCP Consulting, 2020-10-28)People suffering from diabetes or being overweight must severely reduce their sugar use, often seeking food with sweeteners. Often, sugar is replaced by non-nutritious sweeteners in beverages, which also contain several ... -
Application of PHB-liposome particles and nanofibers in cosmetics
(Tanger, 2020-10-01)Our goal was to prepare and characterize PHB containing liposomes and nanofibers for potential application in cosmetic, pharmaceutical and food industry. Prepared particles were stable and had proper properties to be used ... -
Porovnání metod úpravy vzorků pro stanovení makroprvků v mléce technikou ICP-OES
(Slovenská poľnohospodárska univerzita v Nitre, 2020-05-25)Cílem této metodologické studie bylo porovnat několik běžně používaných metod, jako je příprava disperze, solubilizace za použití činidel, mineralizace na mokré či suché cestě a mikrovlnná digesce pro úpravu vzorku před ... -
First Complete Genome of the Thermophilic Polyhydroxyalkanoates Producing Bacterium Schlegelella thermodepolymerans DSM 15344
(Oxford University Press, 2021-01-12)Schlegelella thermodepolymerans is a moderately thermophilic bacterium capable of producing polyhydroxyalkanoates (PHA) – biodegradable polymers representing an alternative to conventional plastics. Here, we present the ... -
Determination of Mercury in Fish Sauces by Thermal Decomposition Gold Amalgamation Atomic Absorption Spectroscopy after Preconcentration by Diffusive Gradients in Thin Films Technique
(MDPI, 2020-12-12)The analysis of mercury in food presents a challenge for analytical chemists. Sample pre-treatment and the preconcentration of mercury prior to measurement are required, even when highly sensitive analytical methods are ... -
Recent Advances in 3D Printing of Polyhydroxyalkanoates: A Review
(Sciendo, 2021-01-22)In the 21st century, additive manufacturing technologies have gained in popularity mainly due to benefits such as rapid prototyping, faster small production runs, flexibility and space for innovations, non-complexity of ... -
Atmospheric Pressure Plasma Polymerized 2-Ethyl-2-oxazoline Based Thin Films for Biomedical Purposes
(MDPI, 2020-11-13)Polyoxazoline thin coatings were deposited on glass substrates using atmospheric pressure plasma polymerization from 2-ethyl-2-oxazoline vapours. The plasma polymerization was performed in dielectric barrier discharge ... -
Revealing the Potential of Lipid and beta-Glucans Coproduction in Basidiomycetes Yeast
(MDPI, 2020-07-08)Beta (beta)-glucans are polysaccharides composed of D-glucose monomers. Nowadays, beta-glucans are gaining attention due to their attractive immunomodulatory biological activities, which can be utilized in pharmaceutical ... -
Synergistic Effect of Chitosan and Selenium Nanoparticles on Biodegradation and Antibacterial Properties of Collagenous Scaffolds Designed for Infected Burn Wounds
(MDPI, 2020-10-05)A highly porous scaffold is a desirable outcome in the field of tissue engineering. The porous structure mediates water-retaining properties that ensure good nutrient transportation as well as creates a suitable environment ... -
A Comparative Study of Some Procedures for Isolation of Fruit DNA of Sufficient Quality for PCR-Based Assays
(MDPI, 2020-09-25)Food fraud has been and still is a problem in the food industry. It is detectable by several approaches, such as high performance liquid chromatography (HPLC), chemometric assays, or DNA-based techniques, each with its own ... -
G-quadruplexes in the Archaea Domain
(MDPI, 2020-09-21)The importance of unusual DNA structures in the regulation of basic cellular processes is an emerging field of research. Amongst local non-B DNA structures, G-quadruplexes (G4s) have gained in popularity during the last ... -
Impact of Microwave Plasma Torch on the Yeast Candida glabrata
(MDPI, 2020-08-11)Recently, various cold plasma sources have been tested for their bactericidal and fungicidal effects with respect to their application in medicine and agriculture. The purpose of this work is to study the effects of a 2.45 ... -
Properties of scaffolds prepared by fused deposition modeling of poly(hydroxyalkanoates)
(Elsevier, 2020-10-15)Poly(hydroxyalkanoates) are biodegradable and biocompatible polymers suitable for tissue engineering. Fused depositon modeling (FDM) belongs to modern rapid prototyping techniques for the fabrication of scaffolds. In this ... -
In-depth Bioiformatic Analyses of Human SARS-CoV-2, SARS-CoV, MERS-CoV, and Other Nidovirales Suggest Important Roles of Noncanonical Nucleic Acid Structures in Their Lifecycles
(Frontiers, 2020-04-03)Noncanonical nucleic acid structures play important roles in the regulation of molecular processess. Considering the importance of the ongoing coronavirus crisis, we decided to evaluate genomes of all coronaviruses sequences ... -
Active biodegradable packaging films modified with grape seeds lignin
(The Royal Society of Chemistry, 2020-08-07)Biodegradable packaging materials represent one possible solution for how to reduce the negative environmental impact of plastics. The main idea of this work was to investigate the possibility of utilizing grape seed lignin ... -
Biotechnological Production of Poly(3-Hydroxybutyrate-co-4-Hydroxybutyrate-co-3-Hydroxyvalerate) Terpolymer by Cupriavidus sp. DSM 19379
(MDPI, 2019-08-26)The terpolymer of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 4-hydroxybutyrate (4HB) was produced employing Cupriavidus sp. DSM 19379. Growth in the presence of -butyrolactone, -caprolactone, 1,4-butanediol, and ... -
Pilot-scale production of poly--hydroxybutyrate with the cyanobacterium Synechocytis sp. CCALA192 in a non-sterile tubular photobioreactor
(Elsevier, 2018-07-20)The biopolymer poly--hydroxybutyrate (PHB) can be used as a promising bioplastic. It has a broad range of applications and is degraded relatively rapidly by soil organisms. Like many prokaryotes, the cyanobacterium ... -
Enzymatic Hydrolysis of Poly(3-hydroxybutyrate-co-Hydroxyvalerate) Scaffolds
(MDPI, 2020-07-05)Polyhydroxyalkanoates (PHAs) are hydrolyzable bio-polyesters. The possibility of utilizing lignocellulosic waste by-products and grape pomace as carbon sources for PHA biosynthesis was investigated. PHAs were biosynthesized ...
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