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Charakterizace skladby mikroflóry a přítomnosti vybraných genů antibiotické rezistence ve vodách pro převoz okrasných ryb

dc.contributor.authorGeržová, Lenkacs
dc.contributor.authorVídeňská, Petracs
dc.contributor.authorFaldynová, Marcelacs
dc.contributor.authorSedlář, Karelcs
dc.contributor.authorProvazník, Ivocs
dc.contributor.authorČížek, Aloiscs
dc.contributor.authorRychlík, Ivancs
dc.date.accessioned2017-07-03T10:46:42Z
dc.date.available2017-07-03T10:46:42Z
dc.date.issued2014-08-01cs
dc.identifier.citationPLOS ONE. 2014, vol. 9, issue 8, p. e103865-e103865.en
dc.identifier.issn1932-6203cs
dc.identifier.other108700cs
dc.identifier.urihttp://hdl.handle.net/11012/69203
dc.description.abstractInternational trade with ornamental fish is gradually recognized as an important source of a wide range of different antibiotic resistant bacteria. In this study we therefore characterized the prevalence of selected antibiotic resistance genes in the microbiota found in the carriage water of ornamental fish originating from 3 different continents. Real-time PCR quantification showed that the sul1 gene was present in 11 out of 100 bacteria. tet(A) was present in 6 out of 100 bacteria and strA, tet(G), sul2 and aadA were present in 1–2 copies per 100 bacteria. Class I integrons were quite common in carriage water microbiota, however, pyrosequencing showed that only 12 different antibiotic gene cassettes were present in class I integrons. The microbiota characterized by pyrosequencing of the V3/V4 variable region of 16S rRNA genes consisted of Proteobacteria (48%), Bacteroidetes (29.5%), Firmicutes (17.8%), Actinobacteria (2.1%) and Fusobacteria (1.6%). Correlation analysis between antibiotic resistance gene prevalence and microbiota composition verified by bacterial culture showed that major reservoirs of sul1 sul2, tet(A), tet(B) tet(G), cat, cml, bla, strA, aacA, aph and aadA could be found among Alpha-, Beta- and Gammaproteobacteria with representatives of Enterobacteriaceae, Pseudomonadaceae, Rhizobiaceae and Comamonadaceae being those most positively associated with the tested antibiotic resistance genes.en
dc.description.abstractMezinárodní obchod s okrasnými rybami je významný zdroj velkého množství antibioticky rezistenstních bakterií. V této studii jsme charakterizovali vybrané geny antibiotické rezistence v mikroflóře z vody použité pro převoz okrasných ryb ze tří různých kontinentů. Reali-time PCR analýza ukázala především velkou abundanci genů sul1 a tet(A).cs
dc.formattextcs
dc.format.extente103865-e103865cs
dc.format.mimetypeapplication/pdfcs
dc.language.isoencs
dc.publisherPublic Library of Sciencecs
dc.relation.ispartofPLOS ONEcs
dc.relation.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0103865cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectmetagenomicsen
dc.subjectbacteriaen
dc.subjectmicrobiotaen
dc.subjectfishen
dc.subjectmetagenomika
dc.subjectbakterie
dc.subjectmikroflóra
dc.subjectryby
dc.titleCharacterization of Microbiota Composition and Presence of Selected Antibiotic Resistance Genes in Carriage Water of Ornamental Fishen
dc.title.alternativeCharakterizace skladby mikroflóry a přítomnosti vybraných genů antibiotické rezistence ve vodách pro převoz okrasných rybcs
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrstvícs
sync.item.dbidVAV-108700en
sync.item.dbtypeVAVen
sync.item.insts2019.06.17 10:22:08en
sync.item.modts2019.05.18 00:43:41en
dc.coverage.issue8cs
dc.coverage.volume9cs
dc.identifier.doi10.1371/journal.pone.0103865cs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1932-6203/cs
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen


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Except where otherwise noted, this item's license is described as Creative Commons Attribution 4.0 International