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dc.contributor.authorLacková, Zuzanacs
dc.contributor.authorCernei, Natalia Vladimirovnacs
dc.contributor.authorŠtěrbová, Dagmarcs
dc.contributor.authorHaddad, Yazan Abdulmajeed Eyadhcs
dc.contributor.authorRozíková, Veronikacs
dc.contributor.authorKomprda, Tomášcs
dc.contributor.authorZítka, Ondřejcs
dc.date.accessioned2018-08-23T10:52:53Z
dc.date.available2018-08-23T10:52:53Z
dc.date.issued2017-12-31cs
dc.identifier.citationMendelNet 2017. 2017, p. 905-910.en
dc.identifier.isbn978-80-7509-529-9cs
dc.identifier.other148747cs
dc.identifier.urihttp://hdl.handle.net/11012/84181
dc.description.abstractThe first aim of the experiment was an optimization of the method for determination of hydroxyproline in a pig skin using high-performance liquid chromatography with fluorescence detector (HPLC-FLD) and ion-exchange liquid chromatography with VIS detector (IEC-VIS). On the basis of the experiments performed, it was found that HPLC-FLD method is three times more sensitive than IEC-VIS method. For IEC analysis of hydroxyproline, the limit of detection (LOD) was 4.10 g/ml whereas the limit of quantification (LOQ) was 13.50 g/ml. For HPLC analysis of hydroxyproline, the limit of detection (LOD) was 1 ng/ml whereas the limit of quantification (LOQ) was 3 ng/ml. The second aim was the testing of influence of different volume of 6M HCl on extraction of sample (50 mg) for analysis using HPLC-FLD. Here it was found that the best volume was 250 µl 6M HCl.en
dc.formattextcs
dc.format.extent905-910cs
dc.format.mimetypeapplication/pdfcs
dc.language.isoencs
dc.publisherMendel University in Brnocs
dc.relation.ispartofMendelNet 2017cs
dc.relation.urihttps://mendelnet.cz/artkey/mnt-201701-0104_Determination-of-hydroxyproline-using-ion-exchange-liquid-chromatography-with-VIS-detector-and-high-performance.php?back=/magno/mnt/2017/mn1.php?secid=4cs
dc.rights(C) Mendel University in Brnocs
dc.subjectHydroxyprolineen
dc.subjectHPLC-FLDen
dc.subjectIEC-VISen
dc.subjectmicrowave hydrolysisen
dc.subjectpig skinen
dc.titleDetermination of hydroxyproline using ion-exchange liquid chromatography with vis detector and high performance liquid chromatography with fluorescence detectoren
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
sync.item.dbidVAV-148747en
sync.item.dbtypeVAVen
sync.item.insts2019.06.17 10:24:54en
sync.item.modts2019.05.18 00:36:28en
dc.rights.accessopenAccesscs
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen


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