• Bio-Sensing of Cadmium(II) Ions Using Staphylococcus aureus 

    Sochor, Jiří; Zítka, Ondřej; Hynek, David; Krejčová, Ludmila; Trnková, Libuše; Adam, Vojtěch; Hubálek, Jaromír; Kynický, Jindřich; Vrba, Radimír; Kizek, René (MDPI, 2011-11-08)
    Cadmium, as a hazardous pollutant commonly present in the living environment, represents an important risk to human health due to its undesirable effects (oxidative stress, changes in activities of many enzymes, interactions ...
  • Modern Micro and Nanoparticle-Based Imaging Techniques 

    Vaculovičová, Markéta; Pekárková, Jana; Drbohlavová, Jana; Kopel, Pavel; Babula, Petr; Hynek, David; Adam, Vojtěch; Eckschlager, Tomáš; Hubálek, Jaromír; Stiborová, Marie; Kaiser, Jozef; Kizek, René (MDPI, 2012-11-02)
    The requirements for early diagnostics as well as effective treatment of insidious diseases such as cancer constantly increase the pressure on development of efficient and reliable methods for targeted drug/gene delivery ...
  • Behaviour of zinc complexes and zinc sulphide nanoparticles revealed by using screen printed electrodes and spectrometry 

    Nejdl, Lukáš; Ruttkay-Nedecký, Branislav; Kudr, Jiří; Kremplová, Monika; Cernei, Natalia Vladimirovna; Prášek, Jan; Konečná, Marie; Hubálek, Jaromír; Zítka, Ondřej; Kynický, Jindřich; Kopel, Pavel; Kizek, René; Adam, Vojtěch (MDPI AQ, 2013-10-25)
    In this study, we focused on microfluidic electrochemical analysis of zinc complexes (Zn(phen)(his)Cl-2, Zn(his)Cl-2) and ZnS quantum dots (QDs) using printed electrodes. This method was chosen due to the simple (easy to ...
  • Metallothionein – Immunohistochemical Cancer Biomarker: A Meta-Analysis 

    Gumulec, Jaromír; Raudenská, Martina; Adam, Vojtěch; Kizek, René; Masařík, Michal (PLOS, 2014-01-08)
    Metallothionein (MT) has been extensively investigated as a molecular marker of various types of cancer. In spite of the fact that numerous reviews have been published in this field, no meta-analytical approach has been ...
  • Flow Injection Analysis with Electrochemical Detection for Rapid Identification of Platinum-Based Cytostatics and Platinum Chlorides in Water 

    Komínková, Markéta; Heger, Zbyněk; Zítka, Ondřej; Kynický, Jindřich; Pohanka, Miroslav; Beklová, Miroslava; Adam, Vojtěch; Kizek, René (MDPI, 2014-02-04)
    Platinum-based cytostatics, such as cisplatin, carboplatin or oxaliplatin are widely used agents in the treatment of various types of tumors. Large amounts of these drugs are excreted through the urine of patients into ...
  • Influence of Magnetic Microparticles Isolation on Adenine Homonucleotides Structure 

    Kremplová, Monika; Fialová, Dana; Nejdl, Lukáš; Hynek, David; Trnková, Libuše; Hubálek, Jaromír; Kizek, René; Adam, Vojtěch (MDPI, 2014-03-01)
    The electroactivity of purine and pyrimidine bases is the most important property of nucleic acids that is very useful for determining oligonucleotides using square wave voltammetry. This study was focused on the electrochemical ...
  • Interaction of Heavy Metal Ions with Carbon and Iron Based Particles 

    Fialová, Dana; Kremplová, Monika; Melichar, Lukáš; Kopel, Pavel; Hynek, David; Adam, Vojtěch; Kizek, René (MDPI, 2014-03-18)
    Due to the rapid development of industry and associated production of toxic waste, especially heavy metals, there is a great interest to create and upgrade new sorption materials to remove these pollutants from the ...
  • From chip-in-a-lab to lab-on-a-chip: towards a single handheld electronic system for multiple application-specific lab-on-a-chip (ASLOC) 

    Neužil, Pavel (Royal Society of Chemistry, 2014-03-25)
    We present a portable, battery-operated and application-specific lab-on-a-chip (ASLOC) system that can be easily configured for a wide range of lab-on-a-chip applications. It is based on multiplexed electrical current ...
  • Trithiocyanurate Complexes of Iron, Manganese and Nickel and Their Anticholinesterase Activity 

    Kopel, Pavel; Doležal, Karel; Langer, Vratislav; Jun, Daniel; Adam, Vojtěch; Kuča, Kamil; Kizek, René (MDPI, 2014-04-08)
    The complexes of Fe(II), Mn(II) and Ni(II) with a combination of a Schiff base, nitrogen-donor ligand or macrocyclic ligand and trithiocyanuric acid (ttcH3) were prepared and characterized by elemental analysis and ...
  • Algal Biomass Analysis by Laser-Based Analytical Techniques - A Review 

    Pořízka, Pavel; Prochazková, Petra; Prochazka, David; Sládková, Lucia; Novotný, Jan; Petrilak, Michal; Brada, Michal; Samek, Ota; Pilát, Zdeněk; Zemánek, Pavel; Adam, Vojtěch; Kizek, René; Novotný, Karel; Kaiser, Jozef (MDPI AG, 2014-09-23)
    Algal biomass that is represented mainly by commercially grown algal strains has recently found many potential applications in various fields of interest. Its utilization has been found advantageous in the fields of ...
  • Simultaneous Automatic Electrochemical Detection of Zinc, Cadmium, Copper and Lead Ions in Environmental Samples Using a Thin-Film Mercury Electrode and an Artificial Neural Network 

    Kudr, Jiří; Nguyen, Hoai Viet; Gumulec, Jaromír; Nejdl, Lukáš; Blažková, Iva; Ruttkay-Nedecký, Branislav; Hynek, David; Kynický, Jindřich; Adam, Vojtěch; Kizek, René (MDPI, 2015-01-01)
    In this study a device for automatic electrochemical analysis was designed. A three electrodes detection system was attached to a positioning device, which enabled us to move the electrode system from one well to another ...
  • Nanoliter-sized overheated reactor 

    Neužil, Pavel; Sun, Wanxin; Karásek, Tomáš; Manz, Andreas (AIP Publishing, 2015-01-16)
    We report on a microfluidic system formed by 200 nl water droplets, encapsulated by a 600 nl mineral oil placed on a hydrophobically coated glass microscope cover slip. The micromachined heater underneath the glass was ...
  • A 3D Microfluidic Chip for Electrochemical Detection of Hydrolysed Nucleic Bases by a Modified Glassy Carbon Electrode 

    Vlachová, Jana; Tmejová, Kateřina; Kopel, Pavel; Korabík, Michal; Zítka, Jan; Hynek, David; Kynický, Jindřich; Adam, Vojtěch; Kizek, René (MDPI AG, POSTFACH, CH-4005 BASEL, SWITZERLAND, 2015-02-01)
    Modification of carbon materials, especially graphene-based materials, has wide applications in electrochemical detection such as electrochemical lab-on-chip devices. A glassy carbon electrode (GCE) modified with chemically ...
  • Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection 

    Heger, Zbyněk; Cernei, Natalia Vladimirovna; Křížková, Soňa; Masařík, Michal; Kopel, Pavel; Hodek, Petr; Zítka, Ondřej; Adam, Vojtěch; Kizek, René (Nature Publishing Group, 2015-03-09)
    Herein, we describe an ultrasensitive specific biosensing system for detection of sarcosine as a potential biomarker of prostate carcinoma based on Forster resonance energy transfer (FRET). The FRET biosensor employs ...
  • Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients Swab 

    Komínková, Markéta; Michálek, Petr; Číhalová, Kristýna; Guráň, Roman; Cernei, Natalia Vladimirovna; Nejdl, Lukáš; Šmerková, Kristýna; Dostálová, Simona; Hegerová, Dagmar; Heger, Zbyněk; Veselý, Radek; Gumulec, Jaromír; Kynický, Jindřich; Xhaxhiu, Kledi; Zítka, Ondřej; Adam, Vojtěch; Kizek, René (MDPI, 2015-03-31)
    In this work, we focused on the differences between bacterial cultures of E. coli obtained from swabs of infectious wounds of patients compared to laboratory E. coli. In addition, blocking of the protein responsible for ...
  • Jacks of metal/metalloid chelation trade in plants - an overview 

    Naser A. Anjum; Hasanuzzaman, Mirza; Hossain, Mohammad Anwar; Thangavel, Palaniswamy; Roychoudhury, Aryadeep; Sarvajeet, Singh Gill; Rodrigo, Miguel Ángel Merlos; Adam, Vojtěch; Fujita, Masayuki; Kizek, René; Duarte, Armando Da Costa; Pereira, Eduarda; Ahmad, Iqbal (Frontiers, 2015-04-02)
    Varied environmental compartments including soil sarebeingcontaminatedbyamyriadtoxicmetal(loid)s(hereaftertermedas metal/s )mainlythroughanthropogenicactivities.Thesemetalsmaycontaminatefoodchainandbringirreparableconseq ...
  • Biological Activity and Molecular Structures of Bis(benzimidazole) and Trithiocyanurate Complexes 

    Kopel, Pavel; Wawrzak, Dorota; Langer, Vratislav; Číhalová, Kristýna; Hegerová, Dagmar; Veselý, Radek; Adam, Vojtěch; Kizek, René (MDPI, 2015-06-01)
    1-(1H-Benzimidazol-2-yl)-N-(1H-benzimidazol-2-ylmethyl)methanamine (abb) and 2-(1H-benzimidazol-2-ylmethylsulfanylmethyl)-1H-benzimidazole (tbb) have been prepared and characterized by elemental analysis. These bis(benzimidazoles) ...
  • The Composites of Graphene Oxide with Metal or Semimetal Nanoparticles and Their Effect on Pathogenic Microorganisms 

    Richtera, Lukáš; Hegerová, Dagmar; Číhalová, Kristýna; Kremplová, Monika; Milosavljević, Vedran; Kopel, Pavel; Blažková, Iva; Hynek, David; Adam, Vojtěch; Kizek, René (MDPI, 2015-06-01)
    The present experiment describes a synthesis process of composites based on graphene oxide, which was tested as a carrier for composites of metal- or metalloid-based nanoparticles (Cu, Zn, Mn, Ag, AgP, Se) and subsequently ...
  • Papillomavirus infection of roe deer in the Czech Republic and fibropapilloma-associated levels of metallothionein, zinc, and oxidative stress 

    Král, Jiří; Banďouchová, Hana; Brichta, Jiří; Kovacova, Veronika; Ondráček, Karel; Osičková, Jitka; Hrubá, Hana; Hutařová, Zdeňka; Komínková, Markéta; Cernei, Natalia Vladimirovna; Konečná, Marie; Tmejová, Kateřina; Zítka, Ondřej; Adam, Vojtěch; Kízek, René; Skoric, Misa; Tremlová, Bohuslava; Treml, František; Pikula, Jiří (University of Veterinary and Pharmaceutical Sciences, 2015-06-01)
    The present study aimed at the aetiological diagnosis of skin tumours of roe deer (Capreolus capreolus) in the Czech Republic. A total of 33 roe deer specimens showing skin masses were sampled for histopathology, virus ...
  • Single Fluorescence Channel-based Multiplex Detection of Avian Influenza Virus by Quantitative PCR with Intercalating Dye 

    Christian D. Ahrberg; Manz, Andreas; Neužil, Pavel (Nature Publishing Group, 2015-06-19)
    Since its invention in 1985 the polymerase chain reaction (PCR) has become a well-established method for amplification and detection of segments of double-stranded DNA. Incorporation of fluorogenic probe or DNA intercalating ...