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dc.contributor.authorSvoboda, O.
dc.contributor.authorFohlerová, Z.
dc.date.accessioned2015-08-25T08:43:06Z
dc.date.available2015-08-25T08:43:06Z
dc.date.issued2015cs
dc.identifier.citationProceedings of the 21st Conference STUDENT EEICT 2015. s. 491-494. ISBN 978-80-214-5148-3cs
dc.identifier.isbn978-80-214-5148-3
dc.identifier.urihttp://hdl.handle.net/11012/43049
dc.description.abstractThe main disadvantage of long-term storage of living cells in liquid nitrogen is its relatively fast evaporation. For this reason, it is necessary to refill Dewar flask quite often. In our experiments, we used low temperature freezer (-80 °C) as more economical option to liquid nitrogen and we would like to show that there is no significant influence of long-term storage of mammalian cell-sat a low temperature. The effect of temperature was studied as the electrophysiological characteristic of the whole-cell Ca2+ current in HEK293 cells. The current responses were measured from cells stored for eight months at -80 °C and compared with previously published papers. The current traces and I-V curves showed that there are no changes in current response between cells frozen at a low temperature and previously published results. From our results can be concluded that the low temperature freezer is an adequate option for storage of mammalian cells for a couple of months.en
dc.formattextcs
dc.format.extent491-494cs
dc.format.mimetypeapplication/pdfen
dc.language.isoencs
dc.publisherVysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologiícs
dc.relation.ispartofProceedings of the 21st Conference STUDENT EEICT 2015en
dc.relation.urihttp://www.feec.vutbr.cz/EEICT/cs
dc.rights© Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologiícs
dc.subjectpatch clampen
dc.subjectcurrent responseen
dc.subjectcell freezingen
dc.subjectHEK293en
dc.titleThe effect of cryopreservation on the current response of CaV 3.1 transfected HEK293 cellsen
eprints.affiliatedInstitution.departmentFakulta elektrotechniky a komunikačních technologiícs
but.event.date23.04.2015cs
but.event.titleStudent EEICT 2015cs
dc.rights.accessopenAccessen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen


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