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dc.contributor.authorKrál, Jiřícs
dc.contributor.authorBanďouchová, Hanacs
dc.contributor.authorBrichta, Jiřícs
dc.contributor.authorKovacova, Veronikacs
dc.contributor.authorOndráček, Karelcs
dc.contributor.authorOsičková, Jitkacs
dc.contributor.authorHrubá, Hanacs
dc.contributor.authorHutařová, Zdeňkacs
dc.contributor.authorKomínková, Markétacs
dc.contributor.authorCernei, Natalia Vladimirovnacs
dc.contributor.authorKonečná, Mariecs
dc.contributor.authorTmejová, Kateřinacs
dc.contributor.authorZítka, Ondřejcs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorKízek, Renécs
dc.contributor.authorSkoric, Misacs
dc.contributor.authorTremlová, Bohuslavacs
dc.contributor.authorTreml, Františekcs
dc.contributor.authorPikula, Jiřícs
dc.date.accessioned2017-01-20T10:57:05Z
dc.date.available2017-01-20T10:57:05Z
dc.date.issued2015-06-01cs
dc.identifier.citationACTA VETERINARIA BRNO. 2015, vol. 84, issue 2, p. 105-111.en
dc.identifier.issn0001-7213cs
dc.identifier.other123336cs
dc.identifier.urihttp://hdl.handle.net/11012/63714
dc.description.abstractThe present study aimed at the aetiological diagnosis of skin tumours of roe deer (Capreolus capreolus) in the Czech Republic. A total of 33 roe deer specimens showing skin masses were sampled for histopathology, virus detection and identification using polymerase chain reaction (PCR) and deoxyribonucleic acid sequence (DNA) analysis, and to investigate fibropapilloma-associated levels of metallothionein, zinc and oxidative stress in 2012 and 2013. Ticks (Ixodes ricinus) and deer keds (Lipoptena cervi) were also collected and pooled from sampled game specimens. Ticks found on dogs in hunting grounds under study were pooled into another sample. Skin tumours, ranging from 2 to 10 per inspected specimen and 2 to 5 cm in size, were classified as fibropapillomas by histopathology. All 33 viral-infection-suspected skin samples from roe deer in South Moravia and South Bohemia were PCR positive. The nucleotide sequences of PCR products were 100% homologous to the Western roe deer papillomavirus 1 isolate CcPV-1. Ticks and deer keds from positive roe deer and ticks from dogs were PCR and DNA sequence positive for the roe deer papillomavirus. Viral DNA was also demonstrated in one blood sample from a roe deer female. Differences in metallothionein, zinc, taurine and electrochemical index among samples from lesions and normal skin of affected roe deer and negative controls from papillomavirus-non-infected animals were non-significant. While we have demonstrated circulation of specific roe deer papillomavirus in the Czech Republic that results in multiple fibropapillomatous skin tumours, many ecological and epidemiological issues of this wildlife disease still remain unanswered.en
dc.formattextcs
dc.format.extent105-111cs
dc.format.mimetypeapplication/pdfcs
dc.language.isoencs
dc.publisherUniversity of Veterinary and Pharmaceutical Sciencescs
dc.relation.ispartofACTA VETERINARIA BRNOcs
dc.relation.urihttps://actavet.vfu.cz/84/2/0105/cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectCapreolus capreolusen
dc.subjectLipoptena cervien
dc.subjectIxodes ricinusen
dc.subjectpapillomatosisen
dc.subjectskin tumoursen
dc.subjecthunting dogen
dc.titlePapillomavirus infection of roe deer in the Czech Republic and fibropapilloma-associated levels of metallothionein, zinc, and oxidative stressen
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
sync.item.dbidVAV-123336en
sync.item.dbtypeVAVen
sync.item.insts2019.04.03 04:44:03en
sync.item.modts0000.00.00 00:00:00en
dc.coverage.issue2cs
dc.coverage.volume84cs
dc.identifier.doi10.2754/avb201584020105cs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/0001-7213/cs
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen


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Creative Commons Attribution 4.0 International
Except where otherwise noted, this item's license is described as Creative Commons Attribution 4.0 International