Characterization of upconversion nanoparticles by fluorescence spectrometry and capillary electrophoresis
Alternativní metriky PlumXhttp://hdl.handle.net/11012/84178
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Upconversion nanoparticles (UCNPs) are a novel class of luminescent tags for applications in life and material sciences. Unlike traditional fluorophores, UCNPs exhibit emission of shorter wavelength under near-infrared excitation (typically 980 nm). In this work, we have examined these unique photophysical properties by fluorescence spectrometry and capillary electrophoresis. UCNPs co-doped with Yb(III) and Er(III) were characterized using laboratory-made fluorescence spectrometer. We have exploited and evaluated two excitation sources and the dependence of the fluorescence of UCNPs on the relative excitation power. Moreover, capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection was for the first time used for characterization of the nanoparticles. It was proved that CE-LIF is a valuable method to be used for investigation of upconversion luminiscence and monitoring of the interactions of UCNPs with other molecules of interest.
Typ dokumentuRecenzovaný dokument
Zdrojový dokumentMendelNet 2017. 2017, p. 953-957.
- Chytré nanonástroje