DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for Alagille syndrome
Date
2021-02-26
Authors
Hankeová, Simona
Šalplachta, Jakub
Zikmund, Tomáš
Kavková, Michaela
Van Hul, Noemi
Břínek, Adam
Smékalová, Veronika
Lázňovský, Jakub
Dawit, Feven
Jaroš, Josef
ORCID
Advisor
Referee
Mark
Journal Title
Journal ISSN
Volume Title
Publisher
ELIFE SCIENCES PUBLICATIONS LTD
Altmetrics
Abstract
Organ function depends on tissues adopting the correct architecture. However, insights into organ architecture are currently hampered by an absence of standardized quantitative 3D analysis. We aimed to develop a robust technology to visualize, digitalize, and segment the architecture of two tubular systems in 3D: double resin casting micro computed tomography (DUCT). As proof of principle, we applied DUCT to a mouse model for Alagille syndrome (Jag1Ndr/Ndr mice), characterized by intrahepatic bile duct paucity, that can spontaneously generate a biliary system in adulthood. DUCT identified increased central biliary branching and peripheral bile duct tortuosity as two compensatory processes occurring in distinct regions of Jag1Ndr/Ndr liver, leading to full reconstitution of wild-type biliary volume and phenotypic recovery. DUCT is thus a powerful new technology for 3D analysis, which can reveal novel phenotypes and provide a standardized method of defining liver architecture in mouse models.
Description
Citation
Document type
Peer-reviewed
Document version
Published version
Date of access to the full text
Language of document
en