Synthesis of core/shell quantum dots for diagnostic

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Date
2015
ORCID
Advisor
Referee
Mark
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Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologií
Abstract
In this paper, synthesis of colloidal core and core/shell quantum dots (QDs) was described. First, CdTe QDs capped with glutathione, thioglycolic or mercaptopropionic acid were prepared in aqueous phase, and used for synthesis of colloidal core/shell CdTe/ZnS QDs. Core/shell QDs were used for conjugation with bovine serum albumin (BSA) or immunoglobulin G (IgG) via different crosslinkers (CDI, EDC/NHS, EDC). QDs as well as QDs-protein/antibody conjugates were characterized via UV-Vis spectroscopy and capillary electrophoresis (CE). Based on UV-Vis spectroscopy results it was found that, with increasing concentration of BSA, fluorescence intensity of QDs decreased. CE confirmed formation of QDs-BSA and QDs-IgG conjugates.
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Citation
Proceedings of the 21st Conference STUDENT EEICT 2015. s. 371-375. ISBN 978-80-214-5148-3
http://www.feec.vutbr.cz/EEICT/
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Peer-reviewed
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en
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© Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologií
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