Doubling Throughput of a Real-Time PCR

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Date
2015-07-10
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Mark
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Macmillan Publishers
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Abstract
We added an intercallating dye to probe-based real-time PCR. One set of oligonucleotides had FAM probe while the second one was without. We were able to extract two DNA amplification curves using a single fluorescence channel
Přidali jsme interkalující barvivo, aby sonda na bázi real-time PCR. Jedna sada oligonukleotidů mělo FAM sondu, zatímco druhý byl bez. Byli jsme schopni získat dva amplifikace DNA křivky pomocí jednoho fluorescenčním kanálu
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Citation
Scientific Reports. 2015, vol. 15, issue 10, p. 1-10.
http://www.nature.com/articles/srep12595
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Peer-reviewed
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Published version
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en
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Creative Commons Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
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