Characterization of Microbiota Composition and Presence of Selected Antibiotic Resistance Genes in Carriage Water of Ornamental Fish
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Date
2014-08-01
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Mark
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Public Library of Science
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Abstract
International trade with ornamental fish is gradually recognized as an important source of a wide range of different antibiotic resistant bacteria. In this study we therefore characterized the prevalence of selected antibiotic resistance genes in the microbiota found in the carriage water of ornamental fish originating from 3 different continents. Real-time PCR quantification showed that the sul1 gene was present in 11 out of 100 bacteria. tet(A) was present in 6 out of 100 bacteria and strA, tet(G), sul2 and aadA were present in 1–2 copies per 100 bacteria. Class I integrons were quite common in carriage water microbiota, however, pyrosequencing showed that only 12 different antibiotic gene cassettes were present in class I integrons. The microbiota characterized by pyrosequencing of the V3/V4 variable region of 16S rRNA genes consisted of Proteobacteria (48%), Bacteroidetes (29.5%), Firmicutes (17.8%), Actinobacteria (2.1%) and Fusobacteria (1.6%). Correlation analysis between antibiotic resistance gene prevalence and microbiota composition verified by bacterial culture showed that major reservoirs of sul1 sul2, tet(A), tet(B) tet(G), cat, cml, bla, strA, aacA, aph and aadA could be found among Alpha-, Beta- and Gammaproteobacteria with representatives of Enterobacteriaceae, Pseudomonadaceae, Rhizobiaceae and Comamonadaceae being those most positively associated with the tested antibiotic resistance genes.
Mezinárodní obchod s okrasnými rybami je významný zdroj velkého množství antibioticky rezistenstních bakterií. V této studii jsme charakterizovali vybrané geny antibiotické rezistence v mikroflóře z vody použité pro převoz okrasných ryb ze tří různých kontinentů. Reali-time PCR analýza ukázala především velkou abundanci genů sul1 a tet(A).
Mezinárodní obchod s okrasnými rybami je významný zdroj velkého množství antibioticky rezistenstních bakterií. V této studii jsme charakterizovali vybrané geny antibiotické rezistence v mikroflóře z vody použité pro převoz okrasných ryb ze tří různých kontinentů. Reali-time PCR analýza ukázala především velkou abundanci genů sul1 a tet(A).
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PLOS ONE. 2014, vol. 9, issue 8, p. e103865-e103865.
http://europepmc.org/articles/PMC4118911?pdf=render
http://europepmc.org/articles/PMC4118911?pdf=render
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Peer-reviewed
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en