Lactoferrin Isolation Using Monolithic Column Coupled with Spectrometric or Micro-Amperometric Detector
Loading...
Date
2008-01-24
ORCID
Advisor
Referee
Mark
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Altmetrics
Abstract
Lactoferrin is a multifunctional protein with antimicrobial activity and others to health beneficial properties. The main aim of this work was to propose easy to use technique for lactoferrin isolation from cow colostrum samples. Primarily we utilized sodium dodecyl sulphate-polyacrylamide gel electrophoresis for isolation of lactoferrin from the real samples. Moreover we tested automated microfluidic Experion electrophoresis system to isolate lactoferrin from the collostrum sample. The well developed signal of lactoferrin was determined with detection limit (3 S/N) of 20 ng/ml. In
spite of the fact that Experion is faster than SDS-PAGE both separation techniques cannot be used in routine analysis. Therefore we have tested third separation technique, ion exchange chromatography, using monolithic column coupled with UV-VIS detector (LCUV-VIS). We optimized wave length (280 nm), ionic strength of the elution solution (1.5 M NaCl) and flow rate of the retention and elution solutions (0.25 ml/min and 0.75 ml/min. respectively).
Description
Citation
Document type
Peer-reviewed
Document version
Published version
Date of access to the full text
Language of document
en