Biomimetic versus Sintered Calcium Phosphates: The in vitro Behavior of Osteoblasts and Mesenchymal Stem Cells

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dc.contributor.authorSadowska, Joanna-Mariacs
dc.contributor.authorGuillem-Marti, Jordics
dc.contributor.authorMontufar Jimenez, Edgar Benjamincs
dc.contributor.authorEspanol, Montserratcs
dc.contributor.authorGinebra, Maria-Paucs
dc.coverage.issue23-24cs
dc.coverage.volume23cs
dc.date.accessioned2020-08-04T11:03:38Z
dc.date.available2020-08-04T11:03:38Z
dc.date.issued2017-12-01cs
dc.description.abstractThe fabrication of calcium phosphates using biomimetic routes, namely, precipitation processes at body temperature, results in distinct features compared to conventional sintered calcium phosphate ceramics, such as a high specific surface area (SSA) and micro- or nanometric crystal size. The aim of this article is to analyze the effects of these parameters on cell response, focusing on two bone cell types: rat mesenchymal stem cells (rMSCs) and human osteoblastic cells (SaOS-2). Biomimetic calcium-deficient hydroxyapatite (CDHA) was obtained by a low temperature setting reaction, and -tricalcium phosphate (-TCP) and -tricalcium phosphate were subsequently obtained by sintering CDHA either at 1400 degrees C or 1100 degrees C. Sintered stoichiometric hydroxyapatite (HA) was also prepared using ceramic routes. The materials were characterized in terms of SSA, skeletal density, porosity, and pore size distribution. SaOS-2 cells and rMSCs were seeded either directly on the surfaces of the materials or on glass coverslips subsequently placed on top of the materials to expose the cells to the CaP-induced ionic changes in the culture medium, while avoiding any topography-related effects. CDHA produced higher ionic fluctuations in both cell culture media than sintered ceramics, with a strong decrease of calcium and a release of phosphate. Indirect contact cell cultures revealed that both cell types were sensitive to these ionic modifications, resulting in a decrease in proliferation rate, more marked for CDHA, this effect being more pronounced for rMSCs. In direct contact cultures, good cell adhesion was found on all materials, but, while cells were able to proliferate on the sintered calcium phosphates, cell number was significantly reduced with time on biomimetic CDHA, which was associated to a higher percentage of apoptotic cells. Direct contact of the cells with biomimetic CDHA resulted also in a higher alkaline phosphatase activity for both cell types compared to sintered CaPs, indicating a promotion of the osteoblastic phenotype.en
dc.formattextcs
dc.format.extent1297-1309cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationTISSUE ENGINEERING PART A. 2017, vol. 23, issue 23-24, p. 1297-1309.en
dc.identifier.doi10.1089/ten.tea.2016.0406cs
dc.identifier.issn1937-335Xcs
dc.identifier.other146531cs
dc.identifier.urihttp://hdl.handle.net/11012/84619
dc.language.isoencs
dc.publisherMary Ann Liebertcs
dc.relation.ispartofTISSUE ENGINEERING PART Acs
dc.relation.urihttps://www.liebertpub.com/doi/abs/10.1089/ten.tea.2016.0406cs
dc.rights(C) Mary Ann Liebertcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1937-335X/cs
dc.subjectcalcium phosphateen
dc.subjectbiomimetic hydroxyapatiteen
dc.subjectmesenchymal stem cellen
dc.subjectosteoblasten
dc.titleBiomimetic versus Sintered Calcium Phosphates: The in vitro Behavior of Osteoblasts and Mesenchymal Stem Cellsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionsubmittedVersionen
sync.item.dbidVAV-146531en
sync.item.dbtypeVAVen
sync.item.insts2020.08.04 13:03:38en
sync.item.modts2020.08.04 12:19:26en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Charakterizace materiálů a pokročilé povlaky 1-06cs
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