Metallothionein sensing using molecularly imprinted polymers coupled laser ablation inductively coupled plasma-mass spectrometry

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dc.contributor.authorZemánková, Kristýnacs
dc.contributor.authorPavelicová, Kristýnacs
dc.contributor.authorBezděková, Jaroslavacs
dc.contributor.authorNejdl, Lukášcs
dc.contributor.authorVejvodová, Markétacs
dc.contributor.authorVaculovič, Tomášcs
dc.contributor.authorVaculovičová, Markétacs
dc.date.accessioned2021-07-13T14:56:37Z
dc.date.available2021-07-13T14:56:37Z
dc.date.issued2021-07-12cs
dc.description.abstractIn this work, we report a facile method for a detection of mettalothionein (MT) - potential biomarker of tumor diseases. Combination of laser ablation inductively coupled plasma coupled to mass spectrometric detection (LA-ICP-MS) with sample pretreatment by magnetic particles modified by molecularly imprinted polymers (Mag-MIP) enables selective and sensitive protein detection and quantification. After Mag-MIP MT extraction, elements specific for the protein (S, Zn and Cd) were detected in healthy pig skin and pig melanoma tissue using MeLiM (Melanoma-bearing-Libechov-Minipig) model. It was found that levels of detected elements were significantly elevated in melanoma tissue compared to healthy skin. Currently, the investigation of MT dimerization in cancer progression is carried out taking advantage from easily controllable in vitro MT dimerization which is used for simple preparation of selective MIP extraction phase. Unlike other recognition elements, which are not always available for target analyte (e.g. antibodies), MIPs can be easily prepared as required. Moreover, magnetic particles as a MIP substrate enable elegant extraction approach and LA-ICP-MS provides extremely sensitive detection. Therefore, Mag-MIP-LA-ICP-MS is a unique cross-border combination of molecular and elemental analysis.en
dc.formattextcs
dc.format.extent385-389cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationProceedings 12th International Conference on Nanomaterials - Research & Application. 2021, p. 385-389.en
dc.identifier.doi10.37904/nanocon.2020.3749cs
dc.identifier.isbn978-80-87294-98-7cs
dc.identifier.issn2694-930Xcs
dc.identifier.other172000cs
dc.identifier.urihttp://hdl.handle.net/11012/200490
dc.language.isoencs
dc.publisherTangercs
dc.relation.ispartofProceedings 12th International Conference on Nanomaterials - Research & Applicationcs
dc.relation.urihttps://www.confer.cz/nanocon/2020/3749-mettalothionein-sensing-using-molecularly-imprinted-polymers-coupled-to-laser-ablation-inductively-coupled-plasma-mass-spectrometrycs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2694-930X/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectMetallothioneinen
dc.subjectMolecularly imprinted polymersen
dc.subjectMagnetic particlesen
dc.subjectLA-ICP-MSen
dc.titleMetallothionein sensing using molecularly imprinted polymers coupled laser ablation inductively coupled plasma-mass spectrometryen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-172000en
sync.item.dbtypeVAVen
sync.item.insts2021.11.22 00:54:40en
sync.item.modts2021.11.22 00:19:03en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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