In Vitro Interactions between 17 beta-Estradiol and DNA Result in Formation of the Hormone-DNA Complexes

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dc.contributor.authorHeger, Zbyněkcs
dc.contributor.authorGuráň, Romancs
dc.contributor.authorZítka, Ondřejcs
dc.contributor.authorBeklová, Miroslavacs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorKizek, Renécs
dc.coverage.issue8cs
dc.coverage.volume11cs
dc.date.accessioned2020-08-04T11:03:48Z
dc.date.available2020-08-04T11:03:48Z
dc.date.issued2014-08-01cs
dc.description.abstractBeyond the role of 17 beta-estradiol (E-2) in reproduction and during the menstrual cycle, it has been shown to modulate numerous physiological processes such as cell proliferation, apoptosis, inflammation and ion transport in many tissues. The pathways in which estrogens affect an organism have been partially described, although many questions still exist regarding estrogens' interaction with biomacromolecules. Hence, the present study showed the interaction of four oligonucleotides (17, 20, 24 and/or 38-mer) with E-2. The strength of these interactions was evaluated using optical methods, showing that the interaction is influenced by three major factors, namely: oligonucleotide length, E-2 concentration and interaction time. In addition, the denaturation phenomenon of DNA revealed that the binding of E-2 leads to destabilization of hydrogen bonds between the nitrogenous bases of DNA strands resulting in a decrease of their melting temperatures (T-m). To obtain a more detailed insight into these interactions, MALDI-TOF mass spectrometry was employed. This study revealed that E-2 with DNA forms non-covalent physical complexes, observed as the mass shifts for app. 270 Da (Mr of E-2) to higher molecular masses. Taken together, our results indicate that E-2 can affect biomacromolecules, as circulating oligonucleotides, which can trigger mutations, leading to various unwanted effects.en
dc.formattextcs
dc.format.extent7725-7739cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationInternational Journal of Environmental Research and Public Health. 2014, vol. 11, issue 8, p. 7725-7739.en
dc.identifier.doi10.3390/ijerph110807725cs
dc.identifier.issn1660-4601cs
dc.identifier.other145195cs
dc.identifier.urihttp://hdl.handle.net/11012/181133
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofInternational Journal of Environmental Research and Public Healthcs
dc.relation.urihttp://www.mdpi.com/1660-4601/11/8/7725cs
dc.rightsCreative Commons Attribution 3.0 Unportedcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1660-4601/cs
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/cs
dc.subjectcanceren
dc.subjectdenaturationen
dc.subjectendocrine disruptorsen
dc.subjectestrogensen
dc.subjectnucleic acidsen
dc.subjectspectrometryen
dc.titleIn Vitro Interactions between 17 beta-Estradiol and DNA Result in Formation of the Hormone-DNA Complexesen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-145195en
sync.item.dbtypeVAVen
sync.item.insts2020.08.04 13:03:48en
sync.item.modts2020.08.04 12:27:43en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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