Sarcosine up-regulates expression of genes involved in cell cycle progression of metastatic models of prostate cancer

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dc.contributor.authorHeger, Zbyněkcs
dc.contributor.authorMerlos Rodrigo, Miguel Ángelcs
dc.contributor.authorMichálek, Petrcs
dc.contributor.authorPolanská, Hanacs
dc.contributor.authorMasařík, Michalcs
dc.contributor.authorVít, Vítězslavcs
dc.contributor.authorPlevová, Marianacs
dc.contributor.authorPacík, Daliborcs
dc.contributor.authorEckschlager, Tomášcs
dc.contributor.authorStiborová, Mariecs
dc.contributor.authorAdam, Vojtěchcs
dc.coverage.issue11cs
dc.coverage.volume11cs
dc.date.accessioned2020-08-04T11:04:08Z
dc.date.available2020-08-04T11:04:08Z
dc.date.issued2016-11-08cs
dc.description.abstractThe effects of sarcosine on the processes driving prostate cancer (PCa) development remain still unclear. Herein, we show that a supplementation of metastatic PCa cells (androgen independent PC-3 and androgen dependent LNCaP) with sarcosine stimulates cells proliferation in vitro. Similar stimulatory effects were observed also in PCa murine xenografts, in which sarcosine treatment induced a tumor growth and significantly reduced weight of treated mice (p < 0.05). Determination of sarcosine metabolism-related amino acids and enzymes within tumor mass revealed significantly increased glycine, serine and sarcosine concentrations after treatment accompanied with the increased amount of sarcosine dehydrogenase. In both tumor types, dimethylglycine and glycine-N-methyltransferase were affected slightly, only. To identify the effects of sarcosine treatment on the expression of genes involved in any aspect of cancer development, we further investigated expression profiles of excised tumors using cDNA electrochemical microarray followed by validation using the semi-quantitative PCR. We found 25 differentially expressed genes in PC-3, 32 in LNCaP tumors and 18 overlapping genes. Bioinformatical processing revealed strong sarcosine-related induction of genes involved particularly in a cell cycle progression. Our exploratory study demonstrates that sarcosine stimulates PCa metastatic cells irrespectively of androgen dependence. Overall, the obtained data provides valuable information towards understanding the role of sarcosine in PCa progression and adds another piece of puzzle into a picture of sarcosine oncometabolic potential.en
dc.formattextcs
dc.format.extent1-20cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationPLOS ONE. 2016, vol. 11, issue 11, p. 1-20.en
dc.identifier.doi10.1371/journal.pone.0165830cs
dc.identifier.issn1932-6203cs
dc.identifier.other131991cs
dc.identifier.urihttp://hdl.handle.net/11012/69380
dc.language.isoencs
dc.publisherPLOScs
dc.relation.ispartofPLOS ONEcs
dc.relation.urihttp://europepmc.org/articles/PMC5100880?pdf=rendercs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1932-6203/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectmetabolism-related proteinsen
dc.subjectone-carbon metabolismen
dc.subjectthymidylate synthaseen
dc.subjectbreast-canceren
dc.subjectphosphorylationen
dc.subjectproliferationen
dc.subjectreceptorsen
dc.subjecttherapyen
dc.subjectglycineen
dc.subjectserineen
dc.titleSarcosine up-regulates expression of genes involved in cell cycle progression of metastatic models of prostate canceren
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-131991en
sync.item.dbtypeVAVen
sync.item.insts2020.08.04 13:04:08en
sync.item.modts2020.08.04 12:45:56en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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