Effect of sarcosine dehydrogenase knockdown on sarcosine metabolism-related genes expression

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dc.contributor.authorŠubrtová, Hanacs
dc.contributor.authorŠplíchal, Zbyněkcs
dc.date.accessioned2020-01-24T11:53:34Z
dc.date.available2020-01-24T11:53:34Z
dc.date.issued2019-12-31cs
dc.description.abstractSarcosine is extensively discussed as potential prostate cancer oncometabolite. Sarcosine dehydrogenase (SARDH) is one of the key enzymes involved in sarcosine metabolism that catalyses oxidative demethylation of sarcosine to glycine in mitochondrion. This study investigates the effects of small interfering RNA (siRNA) mediated SARDH knockdown on gene expression of sarcosine metabolism related enzymes: glycine N-methyltransferase (GNMT), peroxisomal sarcosine oxidase (PIPOX) and dimethylglycine dehydrogenase (DMGDH). The effect of SARDH knockdown was studied in three human prostate cell lines (PNT1A, DU-145, PC3) and gene expression was evaluated by quantitative real-time PCR (qPCR). Although the lowest SARDH knockdown was achieved in cancer DU-145 cell line, the highest changes in expression of other sarcosine metabolism genes were detected. In particular, the significant increase in DMGDH and PIPOX expression was observed. Our findings revealed potential stimulation effect of SARDH knockdown on DMGDH and PIPOX expression in prostate cancer cell line DU-145.en
dc.formattextcs
dc.format.extent648-653cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMendelNet 2019 Proceedings of 26th International PhD Students Conference. 2019, p. 648-653.en
dc.identifier.isbn978-80-7509-688-3cs
dc.identifier.other161420cs
dc.identifier.urihttp://hdl.handle.net/11012/184135
dc.language.isoencs
dc.publisherMendel University in Brnocs
dc.relation.ispartofMendelNet 2019 Proceedings of 26th International PhD Students Conferencecs
dc.relation.urihttps://mendelnet.cz/artkey/mnt-201901-0125_Effect-of-sarcosine-dehydrogenase-knockdown-on-sarcosine-metabolism-related-genes-expression.php?back=/magno/mnt/2019/mn1.php?secid=4cs
dc.rights(C) Mendel University in Brnocs
dc.rights.accessopenAccesscs
dc.subjectknock-downen
dc.subjectprostate canceren
dc.subjectsarcosine dehydrogenaseen
dc.subjectsarcosine metabolismen
dc.titleEffect of sarcosine dehydrogenase knockdown on sarcosine metabolism-related genes expressionen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-161420en
sync.item.dbtypeVAVen
sync.item.insts2023.03.02 16:52:38en
sync.item.modts2023.03.02 16:14:27en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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